Abstract
Exposure of the avirulent Kazan 5 treponeme to low concentrations of sodium dodecyl sulfate (1.4 mm) results in the solubilization of the outer envelope. Maximal reaggregation of the outer envelope preparation requires cations, and cation concentration affects both the yield and structure of the aggregates. The reaggregated outer envelope preparation can be resolubilized with the chelating agent, ethylenediaminetetraacetic acid, or with sodium dodecyl sulfate.
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- Auran N. E., Johnson R. C., Ritzi D. M. Isolation of the outer sheath of Leptospira and its immunogenic properties in hamsters. Infect Immun. 1972 Jun;5(6):968–975. doi: 10.1128/iai.5.6.968-975.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Engelman D. M., Terry T. M., Morowitz H. J. Characterization of the plasma membrane of Mycoplasma laidlawii. I. Sodium dodecyl sulfate solubilization. Biochim Biophys Acta. 1967 Jul 3;135(3):381–390. doi: 10.1016/0005-2736(67)90028-4. [DOI] [PubMed] [Google Scholar]
- HARDY P. H., Jr, NELL E. E. Influence of osmotic pressure on the morphology of the Reiter treponeme. J Bacteriol. 1961 Dec;82:967–978. doi: 10.1128/jb.82.6.967-978.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jackson S., Black S. H. Ultrastructure of Treponema pallidum Nichols following lysis by physical and chemical methods. I. Envelope, wall, membrane and fibrils. Arch Mikrobiol. 1971;76(4):308–324. doi: 10.1007/BF00408528. [DOI] [PubMed] [Google Scholar]
- KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ovcinnikov N. M., Delektorskij V. V. Further studies of the morphology of Treponema pallidum under the electron microscope. Br J Vener Dis. 1969 Jun;45(2):87–116. doi: 10.1136/sti.45.2.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pillot J., Ryter A. Structure des spirochètes. 1. Etude des generes Treponema, Borrelia et Leptospira au microscope electronique. Ann Inst Pasteur (Paris) 1965 Jun;108(6):791–804. [PubMed] [Google Scholar]
- Terry T. M., Engelman D. M., Morowitz H. J. Characterization of the plasma membrane of Mycoplasma laidlawii. II. Modes of aggregation of solubilized membrane components. Biochim Biophys Acta. 1967 Jul 3;135(3):391–405. doi: 10.1016/0005-2736(67)90029-6. [DOI] [PubMed] [Google Scholar]