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. 2014 Aug 17;5(18):8665–8680. doi: 10.18632/oncotarget.2356

Figure 3. Withaferin A inhibits self-renewal and pluripotent properties of iCSCL-10A cells.

Figure 3

(A, B) iCSCL-10A cells were treated with the indicated concentrations of WA and ALP activity was measured after 48 hrs. Phase contrast images of ALP staining in iCSCL-10A cells treated with WA (A). Nuclei were counterstained with 40,6-diamidino-2-phenylindole (DAPI). Scale bar, 500 mm. Cell viability was measured after 48 hrs using WST-8 activity assays. Alkaline Phosphatase (ALP) activity was measured after 48 hrs using the TRACP & ALP Assay Kit and a microplate reader. Values represent the mean ± SEM (n = 3) (B). (C, D) iCSCL-10A cells were plated in the presence of the indicated concentrations of WA and tumor sphere formation was assessed after 48 hrs. Phase contrast images of tumor spheres (C). Values represent the mean ± SEM (n = 3) (D). Scale bar, 500 mm.