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. 1973 Mar;7(3):352–355. doi: 10.1128/iai.7.3.352-355.1973

Transmissible Mink Encephalopathy: Studies on the Peripheral Lymphocyte

R F Marsh a, Janice M Miller a,1, R P Hanson a
PMCID: PMC422682  PMID: 4197388

Abstract

Circulating lymphocytes from mink with transmissible mink encephalopathy were studied for the presence of virus particles and for infectivity. Electron microscope examination of phytohemagglutinin-stimulated lymphocyte cultures revealed no evidence of virus-like particles. Circulating lymphocytes were also found to be noninfectious when the transmissible agent could be demonstrated in intact lymphoid organs in infected mink. A lymphopenia was detected in affected mink during the late clinical stage of disease, but this was most likely a secondary effect due to a terminal stress factor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clarke M. C., Haig D. A. Multiplication of scrapie agent in mouse spleen. Res Vet Sci. 1971 Mar;12(2):195–197. [PubMed] [Google Scholar]
  2. Duc-Nguyen H., Henle W. Replication of mumps virus in human leukocyte cultures. J Bacteriol. 1966 Jul;92(1):258–265. doi: 10.1128/jb.92.1.258-265.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  4. Edelman R., Wheelock E. F. Vesicular stomatitis virus replication in human leukocyte cultures: enhancement by phytohemagglutinin. Science. 1966 Nov 25;154(3752):1053–1055. doi: 10.1126/science.154.3752.1053. [DOI] [PubMed] [Google Scholar]
  5. Eklund C. M., Kennedy R. C., Hadlow W. J. Pathogenesis of scrapie virus infection in the mouse. J Infect Dis. 1967 Feb;117(1):15–22. doi: 10.1093/infdis/117.1.15. [DOI] [PubMed] [Google Scholar]
  6. Fraser H., Dickinson A. G. Pathogenesis of scrapie in the mouse: the role of the spleen. Nature. 1970 May 2;226(5244):462–463. doi: 10.1038/226462a0. [DOI] [PubMed] [Google Scholar]
  7. Gardiner A. C. Gel diffusion reactions of tissues and sera from scrapie-affected animals. Res Vet Sci. 1966 Apr;7(2):190–195. [PubMed] [Google Scholar]
  8. Hanson R. P., Eckroade R. J., Marsh R. F., Zu Rhein G. M., Kanitz C. L., Gustafson D. P. Susceptibility of mink to sheep scrapie. Science. 1971 May 21;172(3985):859–861. doi: 10.1126/science.172.3985.859. [DOI] [PubMed] [Google Scholar]
  9. JAHMIAS A. J., KIBRICK S., ROSAN R. C. VIRAL LEUKOCYTE INTERRELATIONSHIPS. I. MULTIPLICATION OF A DNA VIRUS--HERPES SIMPLEX--IN HUMAN LEUKOCYTE CULTURES. J Immunol. 1964 Jul;93:69–74. [PubMed] [Google Scholar]
  10. Lavelle G. C., Sturman L., Hadlow W. J. Isolation from mouse spleen of cell populations with high specific infectivity for scrapie virus. Infect Immun. 1972 Mar;5(3):319–323. doi: 10.1128/iai.5.3.319-323.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Marsh R. F., Hanson R. P. Transmissible mink encephalopathy: neuroglial response. Am J Vet Res. 1969 Sep;30(9):1637–1642. [PubMed] [Google Scholar]
  12. Marsh R. F., Pan I. C., Hanson R. P. Failure to demonstrate specific antibody in transmissible mink encephalopathy. Infect Immun. 1970 Dec;2(6):727–730. doi: 10.1128/iai.2.6.727-730.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miller G., Enders J. F. Vaccinia virus replication and cytopathic effect in cultures in phytohemagglutinin-treated human peripheral blood leukocytes. J Virol. 1968 Aug;2(8):787–792. doi: 10.1128/jvi.2.8.787-792.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miller J. M., Miller L. D., Olson C., Gillette K. G. Virus-like particles in phytohemagglutinin-stimulated lymphocyte cultures with reference to bovine lymphosarcoma. J Natl Cancer Inst. 1969 Dec;43(6):1297–1305. [PubMed] [Google Scholar]
  15. STEMPAK J. G., WARD R. T. AN IMPROVED STAINING METHOD FOR ELECTRON MICROSCOPY. J Cell Biol. 1964 Sep;22:697–701. doi: 10.1083/jcb.22.3.697. [DOI] [PMC free article] [PubMed] [Google Scholar]

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