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. 1973 Mar;7(3):356–360. doi: 10.1128/iai.7.3.356-360.1973

A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lymphogranuloma Venereum Organisms

San-Pin Wang 1, Cho-Chou Kuo 1, J Thomas Grayston 1
PMCID: PMC422683  PMID: 16558075

Abstract

Responding to the pressure of large numbers of trachoma-inclusion conjunctivitis (TRIC)-lymphogranuloma venereum (LGV) isolates from field studies requiring serotyping, we have developed a simplified, less-precise method that utilizes cell culture-grown organisms to produce mouse antisera which is tested agaist prototype TRIC-LGV antigens in the micro-immunofluorescence test. Cell cultures with as few as 5 to 15% of cells showing inclusions produced adequate antibody in mice 4 days after single injection. Knowledge of the reaction of prototype antisera with the antigens has allowed typing of most isolates tested from the pattern of cross-reaction of their antiserum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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