Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1973 Mar;7(3):487–492. doi: 10.1128/iai.7.3.487-492.1973

Immunosorbent for the Isolation of Specific Antibodies Against Mannan: Localization of Antigens in Yeast Cell Walls

H Gerber 1, M Horisberger 1, H Bauer 1
PMCID: PMC422705  PMID: 4123394

Abstract

A procedure is given for the insolubilization of yeast mannan by entrapment in a polyacrylamide gel. Specific anti-mannan antibodies were separated by immunosorption from all other antibodies of a rabbit antiserum against whole, Formalininactivated Candida utilis cells. Colloidal gold coated with the anti-mannan and anti-non-mannan antibody fractions was used for the ultrastructural visualization of cell wall antigens. A control was performed using normal rabbit serum. Inhibition of anti-mannan antibodies was obtained with mannan oligosaccharides, and none of the antibody fractions showed marking of Saccharomyces cerevisiae cell walls.

Full text

PDF
487

Images in this article

489Image
on p.489
489Image
on p.489
490Image
on p.490

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNFELD P., WAN J. ANTIGENS AND ENZYMES MADE INSOLUBLE BY ENTRAPPING THEM INTO LATTICES OF SYNTHETIC POLYMERS. Science. 1963 Nov 8;142(3593):678–679. doi: 10.1126/science.142.3593.678. [DOI] [PubMed] [Google Scholar]
  2. Ballou C. E. A study of the immunochemistry of three yeast mannans. J Biol Chem. 1970 Mar 10;245(5):1197–1203. [PubMed] [Google Scholar]
  3. Bauer H., Horisberger M., Bush D. A., Sigarlakie E. Mannan as a major component of the bud scars of Saccharomyces cerevisiae. Arch Mikrobiol. 1972;85(3):202–208. doi: 10.1007/BF00408845. [DOI] [PubMed] [Google Scholar]
  4. Carrel S., Gerber H., Barandun S. Preparation of polyacrylamide gels which contain protein and their use as high capacity immunosorbents. Nature. 1969 Jan 25;221(5178):385–386. doi: 10.1038/221385a0. [DOI] [PubMed] [Google Scholar]
  5. Crumpton M. J., Parkhouse R. M.E. Comparison of the effects of various detergents on antigen-antibody interaction. FEBS Lett. 1972 May 1;22(2):210–212. doi: 10.1016/0014-5793(72)80047-4. [DOI] [PubMed] [Google Scholar]
  6. Faulk W. P., Taylor G. M. An immunocolloid method for the electron microscope. Immunochemistry. 1971 Nov;8(11):1081–1083. doi: 10.1016/0019-2791(71)90496-4. [DOI] [PubMed] [Google Scholar]
  7. Horisberger M., Bauer H., Bush D. A. Mercury-labelled concanavalin A as a marker in electron microscopy-localisation of mannan in yeast cell walls. FEBS Lett. 1971 Nov 1;18(2):311–314. doi: 10.1016/0014-5793(71)80474-x. [DOI] [PubMed] [Google Scholar]
  8. Kocourek J., Ballou C. E. Method for fingerprinting yeast cell wall mannans. J Bacteriol. 1969 Dec;100(3):1175–1181. doi: 10.1128/jb.100.3.1175-1181.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mill P. J. Phosphomannans and other components of flocculent and non-flocculent walls of Saccharomyces cerevisiae. J Gen Microbiol. 1966 Sep;44(3):329–341. doi: 10.1099/00221287-44-3-329. [DOI] [PubMed] [Google Scholar]
  10. Nagai Y., Hori H. Entrapment of collagen in a polyacrylamide matrix and its application in the purification of animal collagenases. Biochim Biophys Acta. 1972 May 18;263(3):564–573. doi: 10.1016/0005-2795(72)90037-2. [DOI] [PubMed] [Google Scholar]
  11. Suzuki S., Sunayama H., Saito T. Studies on the antigenic activity of yeasts. I. Analysis of the determinant groups of the mannan of Saccharomyces cerevisiae. Jpn J Microbiol. 1968 Mar;12(1):19–24. doi: 10.1111/j.1348-0421.1968.tb00365.x. [DOI] [PubMed] [Google Scholar]
  12. Suzuki S., Sunayama H. Studies on the antigenic activities of yeasts. II. Isolation and inhibition assay of the oligosaccharides from acetolysate of the mannan of Candida albicans. Jpn J Microbiol. 1968 Dec;12(4):413–422. doi: 10.1111/j.1348-0421.1968.tb00414.x. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES