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. 1973 Apr;7(4):567–572. doi: 10.1128/iai.7.4.567-572.1973

Biochemistry of Vibrio cholerae Virulence III. Nutritional Requirements for Toxin Production and the Effects of pH on Toxin Elaboration in Chemically Defined Media

Lynn T Callahan III a,1, Stephen H Richardson a
PMCID: PMC422723  PMID: 4762111

Abstract

The investigations described in this report concern the metabolic and physiologic parameters governing cholera enterotoxin production in chemically defined growth media. The results indicate that the minimal nutritional requirements for growth of pathogenic Vibrio cholerae are the same as those for toxin production, and that toxin production parallels growth of the organisms. Studies of the relationship between toxin accumulation and pH reveal that toxin biosynthesis can be separated from toxin release. Toxin is synthesized below pH 7.0, but release and accumulation of extracellular toxin occur only at neutral or alkaline pH values.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Callahan L. T., 3rd, Ryder R. C., Richardson S. H. Biochemistry of vibrio cholerae virulence. II. Skin permeability factor-cholera enterotoxin production in a chemically defined medium. Infect Immun. 1971 Nov;4(5):611–618. doi: 10.1128/iai.4.5.611-618.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Evans D. J., Jr, Richardson S. H. In vitro production of choleragen and vascular permeability factor by Vibrio cholerae. J Bacteriol. 1968 Jul;96(1):126–130. doi: 10.1128/jb.96.1.126-130.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Finkelstein R. A., LoSpalluto J. J. Production of highly purified choleragen and choleragenoid. J Infect Dis. 1970 May;121(Suppl):63+–63+. doi: 10.1093/infdis/121.supplement.s63. [DOI] [PubMed] [Google Scholar]
  4. Kusama H., Craig J. P. Production of Biologically Active Substances by Two Strains of Vibrio cholerae. Infect Immun. 1970 Jan;1(1):80–87. doi: 10.1128/iai.1.1.80-87.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Richardson S. H., Evans D. G., Feeley J. C. Biochemistry of Vibrio cholerae Virulence I. Purification and Biochemical Properties of PF/Cholera Enterotoxin. Infect Immun. 1970 Jun;1(6):546–554. doi: 10.1128/iai.1.6.546-554.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Richardson S. H. Factors influencing in vitro skin permeability factor production by Vibrio cholerae. J Bacteriol. 1969 Oct;100(1):27–34. doi: 10.1128/jb.100.1.27-34.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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