Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1973 Apr;7(4):604–609. doi: 10.1128/iai.7.4.604-609.1973

Relationship of Adenovirus to Lymphocytes in Naturally Infected Human Tonsils and Adenoids

J Van Der Veen 1, M Lambriex 1
PMCID: PMC422731  PMID: 4796933

Abstract

Purified lymphocytes from human tonsil and adenoid specimens were cultured with and without phytohemagglutinin. Adenovirus was isolated from lymphocytes of 8 of 90 specimens tested. With one exception, it was necessary to culture the lymphocytes before infectious virus could be detected. Phytohemagglutinin stimulation enhanced the recovery of virus. The results suggest that lymphocytes in tonsils and adenoids may be naturally infected with adenovirus and that, in positive cultures, at least 1 of every 107 cells harbors virus or viral precursor at initiation of the cultures. Adenovirus was demonstrated directly in fresh suspensions of unpurified cells from tonsils and adenoids in seven cases. In five of these cases, at least 1 of every 106 cells contained infectious virus. Adenovirus was isolated from 61 (62%) of 98 tonsil and adenoid specimens by the conventional method of tissue fragment culture after various periods of cultivation. The viruses isolated were of serotypes 1, 2, 5, and 6.

Full text

PDF
604

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. August C. S., Merler E., Lucas D. O., Janeway C. A. The response in vitro of human lymphocytes to phytohemagglutinin and to antigens after fractionation on discontinuous density gradients of albumin. Cell Immunol. 1970 Dec;1(6):603–618. doi: 10.1016/0008-8749(70)90026-2. [DOI] [PubMed] [Google Scholar]
  2. Chessin L. N., Börjeson J., Welsh P. D., Douglas S. D., Cooper H. L. Studies on human peripheral blood lymphocytes in vitro. II. Morphological and biochemical studies on the transformation of lymphocytes by pokeweed mitogen. J Exp Med. 1966 Nov 1;124(5):873–884. doi: 10.1084/jem.124.5.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. EVANS A. S. Latent adenovirus infections of the human respiratory tract. Am J Hyg. 1958 May;67(3):256–266. doi: 10.1093/oxfordjournals.aje.a119932. [DOI] [PubMed] [Google Scholar]
  4. Lamvik J. O. Separation of lymphocytes from human blood. Acta Haematol. 1966 May;35(5):294–303. doi: 10.1159/000209135. [DOI] [PubMed] [Google Scholar]
  5. Nász I., Kulcsár G., Dán P., Sallay K. A possible pathogenic role for virus-carrier lymphocytes. J Infect Dis. 1971 Aug;124(2):214–216. doi: 10.1093/infdis/124.2.214. [DOI] [PubMed] [Google Scholar]
  6. Oettgen H. F., Silber R., Miescher P. A., Hirschhorn K. Stimulation of human tonsillar lymphocytes in vitro. Clin Exp Immunol. 1966 Jan;1(1):77–84. [PMC free article] [PubMed] [Google Scholar]
  7. ROWE W. P., HUEBNER R. J., GILMORE L. K., PARROTT R. H., WARD T. G. Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc Soc Exp Biol Med. 1953 Dec;84(3):570–573. doi: 10.3181/00379727-84-20714. [DOI] [PubMed] [Google Scholar]
  8. STROHL W. A., SCHLESINGER R. W. QUANTITATIVE STUDIES OF NATURAL AND EXPERIMENTAL ADENOVIRUS INFECTIONS OF HUMAN CELLS. I. CHARACTERISTICS OF VIRAL MULTIPLICATION IN FIBROBLASTS DERIVED BY LONG-TERM CULTURE FROM TONSILS. Virology. 1965 Jun;26:199–207. doi: 10.1016/0042-6822(65)90047-4. [DOI] [PubMed] [Google Scholar]
  9. STROHL W. A., SCHLESINGER R. W. QUANTITATIVE STUDIES OF NATURAL AND EXPERIMENTAL ADENOVIRUS INFECTIONS OF HUMAN CELLS. II. PRIMARY CULTURES AND THE POSSIBLE ROLE OF ASYNCHRONOUS VIRAL MULTIPLICATION IN THE MAINTENANCE OF INFECTION. Virology. 1965 Jun;26:208–220. doi: 10.1016/0042-6822(65)90048-6. [DOI] [PubMed] [Google Scholar]
  10. VAN DER VEEN J., DIJKMAN J. H. Association of type 21 adenovirus with acute respiratory illness in military recruits. Am J Hyg. 1962 Sep;76:149–159. doi: 10.1093/oxfordjournals.aje.a120270. [DOI] [PubMed] [Google Scholar]
  11. VAN DER VEEN J., KOK G. Isolation and typing of adenoviruses recovered from military recruits with acute respiratory disease in The Netherlands. Am J Hyg. 1957 Mar;65(2):119–129. doi: 10.1093/oxfordjournals.aje.a119860. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES