Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1973 May;7(5):781–785. doi: 10.1128/iai.7.5.781-785.1973

Immune Response of Rats to Group A Streptococcal Vaccine

Gerrie A Leslie 1, Henry F Carwile 1
PMCID: PMC422761  PMID: 4764404

Abstract

Adult female Sprague-Dawley rats were hyperimmunized with group A streptococcal vaccine in an attempt to evaluate this species for its immunological responsiveness to the group A streptococcal carbohydrate. Pronounced hyperproteinemia, generally attributable to hypergammaglobulinemia was produced in 30 to 40% of the animals. The immune response of the rats was extremely variable, yielding precipitating antibody concentrations ranging from <0.1 mg per ml to >26 mg per ml. Antibody levels also varied considerably in the same rat at different times during the immunization routine. The highest concentration of precipitating antibody was 26.3 mg per ml. Evidence is presented to suggest the production of antibodies of restricted heterogeneity and the production of non-streptococcal antibodies during the immune response.

Full text

PDF
781

Images in this article

784Image
on p.784

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BINAGHI R. A., BENACERRAF B. THE PRODUCTION OF ANAPHYLACTIC ANTIBODY IN THE RAT. J Immunol. 1964 Jun;92:920–926. [PubMed] [Google Scholar]
  2. Bokisch V. A., Bernstein D., Krause R. M. Occurrence of 19S and 7S anti-IgGs during hyperimmunization of rabbits with streptococci. J Exp Med. 1972 Oct 1;136(4):799–815. doi: 10.1084/jem.136.4.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Braun D. G., Eichmann K., Krause R. M. Rabbit antibodies to streptococcal carbohydrates. Influence of primary and secondary immunization and of possible genetic factors on the antibody response. J Exp Med. 1969 Apr 1;129(4):809–830. doi: 10.1084/jem.129.4.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Braun D. G., Kindred B., Jacobson E. B. Streptococcal group A carbohydrate antibodies in mice: evidence for strain differences in magnitude and restriction of the response, and for thymus dependence. Eur J Immunol. 1972 Apr;2(2):138–143. doi: 10.1002/eji.1830020209. [DOI] [PubMed] [Google Scholar]
  5. Clem L. W., Leslie G. A. Production of 19S IgM antibodies with restricted heterogeneity from sharks. Proc Natl Acad Sci U S A. 1971 Jan;68(1):139–141. doi: 10.1073/pnas.68.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eichmann K., Braun D. G., Krause R. M. Influence of genetic factors on the magnitude and the heterogeneity of the immune response in the rabbit. J Exp Med. 1971 Jul 1;134(1):48–65. doi: 10.1084/jem.134.1.48. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Frommel D., Dupuy J. M., Litman G. W., Good R. A. Use of immunoadsorption techniques in the preparation of chemical agammaglobulinemia. J Immunol. 1970 Nov;105(5):1292–1293. [PubMed] [Google Scholar]
  8. Hood L., Lackland H., Eichman K., Kindt T. J., Braun D. G., Krause R. M. Amino acid sequence restriction in rabbit antibody light chains. Proc Natl Acad Sci U S A. 1969 Jul;63(3):890–896. doi: 10.1073/pnas.63.3.890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Karakawa W. W., Lackland H., Krause R. M. The production of streptococcal group-specific antibodies in rabbits. I. The identification of groups A and A-variant agglutinins in group A antisera. J Immunol. 1966 Feb;96(2):204–209. [PubMed] [Google Scholar]
  10. Kristiansen T., Sundberg L., Porath J. Studies on blood group substances. II. Coupling of blood group substane A to hydroxyl-containing matrices, including aminoethyl cellulose and agarose. Biochim Biophys Acta. 1969 Jun 17;184(1):93–98. [PubMed] [Google Scholar]
  11. Leslie G. A., Clem L. W. Chicken antibodies to group A streptococcal carbohydrate. J Immunol. 1973 Jan;110(1):191–196. [PubMed] [Google Scholar]
  12. Miller E. J., Osterland C. K., Davie J. M., Krause R. M. Electrophoretic analysis of polypeptide chains isolated from antibodies in the serum of immunized rabbits. J Immunol. 1967 Apr;98(4):710–715. [PubMed] [Google Scholar]
  13. Osterland C. K., Miller E. J., Karakawa W. W., Krause R. M. Characteristics of streptococcal group-specific antibody isolated from hyperimmune rabbits. J Exp Med. 1966 Apr 1;123(4):599–614. doi: 10.1084/jem.123.4.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pincus J. H., Jaton J. C., Bloch K. J., Haber E. Antibodies to type III and type VIII pneumococcal polysaccharides: evidence for restricted structural heterogeneity in hyperimmunized rabbits. J Immunol. 1970 May;104(5):1143–1148. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES