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. 1973 Jul;8(1):4–7. doi: 10.1128/iai.8.1.4-7.1973

Metabolism of Rickettsia tsutsugamushi and Rickettsia rickettsi in Irradiated Host Cells

E Weiss 1, A E Green 1, R Grays 1, L M Newman 1
PMCID: PMC422800  PMID: 4198104

Abstract

The metabolism of Rickettsia tsutsugamushi (Gilliam strain) multiplying in irradiated L cells was investigated by methods involving the use of 14C-labeled substrates and cycloheximide, an inhibitor of eukaryotic metabolism. Cycloheximide-resistant amino acid and adenine incorporations were appreciably higher in infected than in uninfected cultures during the period from 3 to 5 or 6 days postinoculation. The metabolism of R. rickettsi was similarly studied in primary duck embryo cells, which are more susceptible to infection with this rickettsia than are L cells. A difference in cycloheximide-resistant activity between infected and uninfected cultures was also noted, but was small. This finding is attributed to the more limited growth of R. rickettsi.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. R., Hopps H. E., Barile M. F., Bernheim B. C. Comparison of the ultrastructure of several rickettsiae, ornithosis virus, and Mycoplasma in tissue culture. J Bacteriol. 1965 Nov;90(5):1387–1404. doi: 10.1128/jb.90.5.1387-1404.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOZEMAN F. M., HOPPS H. E., DANAUSKAS J. X., JACKSON E. B., SMADEL J. E. Study on the growth of Rickettsiae. I. A tissue culture system for quantitative estimations of Rickettsia tsutsugamushi. J Immunol. 1956 Jun;76(6):475–488. [PubMed] [Google Scholar]
  3. Barker L. F., Patt J. K., Hopps H. E. Titration and neutralization of Rickettsia tsutsugamushi in tissue culture. J Immunol. 1968 Apr;100(4):825–830. [PubMed] [Google Scholar]
  4. Barker L. F., Patt J. K. Production of rickettsial complement-fixing antigens in tissue culture. J Immunol. 1968 Apr;100(4):821–824. [PubMed] [Google Scholar]
  5. COHN Z. A., BOZEMAN F. M., CAMPBELL J. M., HUMPHRIES J. W., SAWYER T. K. Study on growth of Rickettsia. V. Penetration of Rickettsia tsutsugamushi into mammalian cells in vitro. J Exp Med. 1959 Mar 1;109(3):271–292. doi: 10.1084/jem.109.3.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HOPPS H. E., JACKSON E. B., DANAUSKAS J. X., SMADEL J. E. Study on the growth of rickettsiae. III. Influence of extracellular environment on the growth of Rickettsia tsutsugamusni in tissue culture cells. J Immunol. 1959 Feb;82(2):161–171. [PubMed] [Google Scholar]
  7. Kenyon R. H., Acree W. M., Wright G. G., Melchior F. W., Jr Preparation of vaccines for Rocky Mountain spotted fever from rickettsiae propagated in cell culture. J Infect Dis. 1972 Feb;125(2):146–152. doi: 10.1093/infdis/125.2.146. [DOI] [PubMed] [Google Scholar]
  8. McDade J. E., Gerone P. J. Plaque assay for Q fever and scrub typhus rickettsiae. Appl Microbiol. 1970 Jun;19(6):963–965. doi: 10.1128/am.19.6.963-965.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McDade J. E., Stakebake J. R., Gerone P. J. Plaque assay system for several species of Rickettsia. J Bacteriol. 1969 Sep;99(3):910–912. doi: 10.1128/jb.99.3.910-912.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SCHAECHTER M., BOZEMAN F. M., SMADEL J. E. Study on the growth of Rickettsiae. II. Morphologic observations of living Rickettsiae in tissue culture cells. Virology. 1957 Feb;3(1):160–172. doi: 10.1016/0042-6822(57)90030-2. [DOI] [PubMed] [Google Scholar]
  11. Weinberg E. H., Stakebake J. R., Gerone P. J. Plaque assay for Rickettsia rickettsii. J Bacteriol. 1969 May;98(2):398–402. doi: 10.1128/jb.98.2.398-402.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Weiss E., Newman L. W., Grays R., Green A. E. Metabolism of Rickettsia typhi and Rickettsia akari in irradiated L cells. Infect Immun. 1972 Jul;6(1):50–57. doi: 10.1128/iai.6.1.50-57.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wike D. A., Tallent G., Peacock M. G., Ormsbee R. A. Studies of the rickettsial plaque assay technique. Infect Immun. 1972 May;5(5):715–722. doi: 10.1128/iai.5.5.715-722.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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