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. 1973 Aug;8(2):199–207. doi: 10.1128/iai.8.2.199-207.1973

Detection and Characterization of Defective Mycoplasmacidal Antibody Produced by Rodents Against Mycoplasma arthritidis

B C Cole 1, J R Ward 1
PMCID: PMC422833  PMID: 4125266

Abstract

A procedure has been developed for the detection of mycoplasmacidal antibody in the serum of rats and mice infected with Mycoplasma arthritidis. Antibody activity can apparently be measured only by using resting mycoplasma cells. The reaction is complement dependent and is usually complete within 3 h. Early antibody (5 days) could be detected only within the immunoglobulin (Ig)M fraction, whereas late antibody (42 days) is found within the IgG fraction. The mycoplasmacidal antibody was highly specific and cross reactions were not observed with other mycoplasma species. Antibody activity could be removed from serum by absorption with either viable or nonviable M. arthritidis. Cidal antibody produced by rats against other mycoplasma species was active against both resting and multiplying cells. Mycoplasmacidal antibody against M. arthritidis was detected in rats 2 days after injection of the organisms and persisted at least through 300 days. Mice usually developed antibodies by 3 days after injection of the organisms and moderate titers have been observed for as long as 487 days. Since viable mycoplasmas can persist in the peripheral circulation in the presence of antibody, it is likely that viable mycoplasma-antibody complexes are also present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barker L. F., Patt J. K. Role of complement in immune inactivation of Mycoplasma gallisepticum. J Bacteriol. 1967 Aug;94(2):403–408. doi: 10.1128/jb.94.2.403-408.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brunner H., James W. D., Horswood R. L., Chanock R. M. Measurement of Mycoplasma pneumoniae mycoplasmacidal antibody in human serum. J Immunol. 1972 Jun;108(6):1491–1498. [PubMed] [Google Scholar]
  3. Brunner H., Razin S., Kalica A. R., Chanock R. M. Lysis and death of Mycoplasma pneumoniae by antibody and complement. J Immunol. 1971 Apr;106(4):907–916. [PubMed] [Google Scholar]
  4. CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CLYDE W. A., Jr MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA. J Immunol. 1964 Jun;92:958–965. [PubMed] [Google Scholar]
  6. Cahill J. F., Cole B. C., Wiley B. B., Ward J. R. Role of Biological Mimicry in the Pathogenesis of Rat Arthritis Induced by Mycoplasma arthritidis. Infect Immun. 1971 Jan;3(1):24–35. doi: 10.1128/iai.3.1.24-35.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Coke B. C., Ward J. R., Golightly-Rowland C., Trapp G. A. Chronic proliferative arthritis of mice induced by Mycoplasma arthritidis. II. Serological responses of the lost and effect of vaccines. Infect Immun. 1971 Oct;4(4):431–440. doi: 10.1128/iai.4.4.431-440.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cole B. C., Cahill J. F., Wiley B. B., Ward J. R. Immunological responses of the rat to Mycoplasma arthritidis. J Bacteriol. 1969 Jun;98(3):930–937. doi: 10.1128/jb.98.3.930-937.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cole B. C., Golightly-Rowland L., Ward J. R., Wiley B. B. Immunological response of rodents to murine mycoplasmas. Infect Immun. 1970 Oct;2(4):419–425. doi: 10.1128/iai.2.4.419-425.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cole B. C., Ward J. R. Fate of intravenously injected Mycoplasma arthritidis in rodents and effect of vaccines. Infect Immun. 1973 Mar;7(3):416–425. doi: 10.1128/iai.7.3.416-425.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cole B. C., Ward J. R. Interaction of Mycoplasma arthritidis and other mycoplasmas with murine peritoneal macrophages. Infect Immun. 1973 May;7(5):691–699. doi: 10.1128/iai.7.5.691-699.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Cole B. C., Ward J. R., Jones R. S., Cahill J. F. Chronic proliferative arthritis of mice induced by Mycoplasma arthritidis. I. Induction of disease and histopathological characteristics. Infect Immun. 1971 Oct;4(4):344–355. doi: 10.1128/iai.4.4.344-355.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. EDWARD D. G., FITZGERALD W. A. Inhibition of growth of pleuropneumonia-like organisms by antibody. J Pathol Bacteriol. 1954 Jul;68(1):23–30. doi: 10.1002/path.1700680104. [DOI] [PubMed] [Google Scholar]
  14. Fernald G. W., Clyde W. A., Jr, Denny F. W. Factors influencing growth inhibition of Mycoplasma pneumoniae by immune sera. Proc Soc Exp Biol Med. 1967 Oct;126(1):161–166. doi: 10.3181/00379727-126-32391. [DOI] [PubMed] [Google Scholar]
  15. Gale J. L., Kenny G. E. Complement dependent killing of Mycoplasma pneumoniae by antibody: kinetics of the reaction. J Immunol. 1970 May;104(5):1175–1183. [PubMed] [Google Scholar]
  16. Golightly-Rowland L., Cole B. C., Ward J. R., Wiley B. B. Effect of Animal Passage on Arthritogenic and Biological Properties of Mycoplasma arthritidis. Infect Immun. 1970 Jun;1(6):538–545. doi: 10.1128/iai.1.6.538-545.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
  18. Purcell R. H., Taylor-Robinson D., Wong D. C., Chanock R. M. A color test for the measurement of antibody to the non-acid-forming human Mycoplasma species. Am J Epidemiol. 1966 Jul;84(1):51–66. doi: 10.1093/oxfordjournals.aje.a120627. [DOI] [PubMed] [Google Scholar]
  19. Purcell R. H., Taylor-Robinson D., Wong D., Chanock R. M. Color test for the measurement of antibody to T-strain mycoplasmas. J Bacteriol. 1966 Jul;92(1):6–12. doi: 10.1128/jb.92.1.6-12.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Riggs S., Sharp J. T., Carpenter R. R. Factors involved in growth-inhibition of mycoplasma by immune serum. Ann N Y Acad Sci. 1967 Jul 28;143(1):784–793. doi: 10.1111/j.1749-6632.1967.tb27725.x. [DOI] [PubMed] [Google Scholar]
  21. Stanbridge E., Hayflick L. Growth inhibition test for identification of Mycoplasma species utilizing dried antiserum-impregnated paper discs. J Bacteriol. 1967 Apr;93(4):1392–1396. doi: 10.1128/jb.93.4.1392-1396.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J Hyg (Lond) 1966 Mar;64(1):91–104. doi: 10.1017/s0022172400040377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wallis C., Melnick J. L. Virus aggregation as the cause of the non-neutralizable persistent fraction. J Virol. 1967 Jun;1(3):478–488. doi: 10.1128/jvi.1.3.478-488.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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