Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1973 Aug;8(2):222–227. doi: 10.1128/iai.8.2.222-227.1973

In Vitro Migration of Human Alveolar Macrophages: Effects of Cigarette Smoking

Glenn A Warr 1, R Russell Martin 1
PMCID: PMC422836  PMID: 4579901

Abstract

The responsiveness of alveolar macrophages lavaged from healthy volunteers to migration inhibitory factor (MIF) was tested by using the capillary-tube assay method. In every instance, macrophages from nonsmokers responded to MIF as demonstrated by a depression in migration of at least 30%, whereas MIF did not inhibit migration of macrophages from smokers. Cells from smokers migrated at a rate three times faster than cells from nonsmokers. Migration of macrophages from nonsmokers with delayed hypersensitivity skin reactions to Candida albicans antigen was inhibited when antigen was present in the tissue culture medium. Antigen did not inhibit macrophages from subjects who lacked delayed hypersensitivity to the antigen, or from subjects who were cigarette smokers. Since alveolar macrophages can respond to MIF in vitro, they may play a role in cell-mediated immune reactions in the lung. Cigarette smoking may interfere with this participation.

Full text

PDF
222

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartfeld H., Atoynatan T. Cellular immunity. Activity and properties of human migration inhibitory factor. Int Arch Allergy Appl Immunol. 1970;38(5):549–553. [PubMed] [Google Scholar]
  2. Bennett B., Bloom B. R. Studies on the migration inhibitory factor associated with delayed-type hypersensitivity: cytodynamics and specificity. Transplantation. 1967 Jul;5(4 Suppl):996–1000. [PubMed] [Google Scholar]
  3. Bloom B. R., Bennett B. Macrophages and delayed-type hypersensitivity. Semin Hematol. 1970 Apr;7(2):215–224. [PubMed] [Google Scholar]
  4. Bloom B. R., Jimenez L. Migration inhibitory factor and the cellular basis of delayed-type hypersensitivity reactions. Am J Pathol. 1970 Sep;60(3):453–468. [PMC free article] [PubMed] [Google Scholar]
  5. Cohen A. B., Cline M. J. The human alveolar macrophage: isolation, cultivation in vitro, and studies of morphologic and functional characteristics. J Clin Invest. 1971 Jul;50(7):1390–1398. doi: 10.1172/JCI106622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Finley T. N., Swenson E. W., Curran W. S., Huber G. L., Ladman A. J. Bronchopulmonary lavage in normal subjects and patients with obstructive lung disease. Ann Intern Med. 1967 Apr;66(4):651–658. doi: 10.7326/0003-4819-66-4-651. [DOI] [PubMed] [Google Scholar]
  7. Harris J. O., Swenson E. W., Johnson J. E., 3rd Human alveolar macrophages: comparison of phagocytic ability, glucose utilization, and ultrastructure in smokers and nonsmokers. J Clin Invest. 1970 Nov;49(11):2086–2096. doi: 10.1172/JCI106426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kelly M. T., Martin R. R., White A. Mediators of histamine release from human platelets, lymphocytes, and granulocytes. J Clin Invest. 1971 May;50(5):1044–1049. doi: 10.1172/JCI106575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Leu R. W., Eddleston A. L., Hadden J. W., Good R. A. Mechanism of action of migration inhibitory factor (MIF). I. Evidence for a receptor for MIF present on the peritoneal macrophage but not on the alveolar macrophage. J Exp Med. 1972 Sep 1;136(3):589–603. doi: 10.1084/jem.136.3.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martin R. R. Altered morphology and increased acid hydrolase content of pulmonary macrophages from cigarette smokers. Am Rev Respir Dis. 1973 Apr;107(4):596–601. doi: 10.1164/arrd.1973.107.4.596. [DOI] [PubMed] [Google Scholar]
  12. Nathan C. F., Karnovsky M. L., David J. R. Alterations of macrophage functions by mediators from lymphocytes. J Exp Med. 1971 Jun 1;133(6):1356–1376. doi: 10.1084/jem.133.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rocklin R. E., Meyers O. L., David J. R. An in vitro assay for cellular hypersensitivity in man. J Immunol. 1970 Jan;104(1):95–102. [PubMed] [Google Scholar]
  14. Roque A. L., Pickren J. W. Enzymatic changes in fluorescent alveolar macrophages of the lungs of cigarette smokers. Acta Cytol. 1968 Nov-Dec;12(6):420–429. [PubMed] [Google Scholar]
  15. Simon H. B., Sheagren J. N. Enhancement of macrophage bactericidal capacity by antigenically stimulated immune lymphocytes. Cell Immunol. 1972 Jun;4(2):163–174. doi: 10.1016/0008-8749(72)90015-9. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES