Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1973 Sep;8(3):370–380. doi: 10.1128/iai.8.3.370-380.1973

In Vitro Correlates of Delayed Hypersensitivity in Man: Ambiguity of Polymorphonuclear Neutrophils as Indicator Cells in Leukocyte Migration Test

George Senyk 1, W Keith Hadley 1
PMCID: PMC422858  PMID: 4581009

Abstract

Delayed cutaneous hypersensitivity (DCH) of 12 normal adult subjects to purified protein derivative (PPD) of Mycobacterium tuberculosis, streptococcal streptokinase-streptodornase (SK-SD), and Candida albicans Dermatophytin O (DO) was assayed in vivo by skin testing and compared with such in vitro correlates of cellular immunity as lymphocyte transformation (LT) and inhibition of leukocyte migration (ILM) from microcapillary tubes or in agarose gel. LT was shown to be the best in vitro correlate of specific lymphocyte sensitization with all antigens. In the ILM assays, PPD showed good correlation with in vivo DCH and in vitro LT; SK-SD showed partial correlation; DO showed no correlation, not being active in any of the ILM tests. Cell distribution and morphology of stained migration patterns, ILM tests performed on separated populations of lymphocytes and polymorphonuclear leukocytes (PMN), as well as the ability of test antigens to stimulate PMN cells to reduce nitroblue-tetrazolium dye, indicated that in ILM tests mononuclear cells were not inhibited in their migration, whereas migration of PMN cells appeared to depend on their direct reaction with the test antigens.

Full text

PDF
370

Images in this article

373Image
on p.373
374Image
on p.374
375Image
on p.375
376Image
on p.376

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bardana E. J., Jr, McClatchy J. K., Farr R. S., Minden P. Universal occurrence of antibodies to tubercle bacilli in sera from non-tuberculous and tuberculous individuals. Clin Exp Immunol. 1973 Jan;13(1):65–77. [PMC free article] [PubMed] [Google Scholar]
  2. Bendixen G., Soborg M. A leucocyte migration technique for in vitro detection of cellular (delayed type) hypersensitivity in man. Dan Med Bull. 1969 Jan;16(1):1–6. [PubMed] [Google Scholar]
  3. Bloom B. R. In vitro approaches to the mechanism of cell-mediated immune reactions. Adv Immunol. 1971;13:101–208. doi: 10.1016/s0065-2776(08)60184-4. [DOI] [PubMed] [Google Scholar]
  4. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  5. Claflin J. L., Larson C. L. Infection-immunity in tularemia: specificity of cellular immunity. Infect Immun. 1972 Mar;5(3):311–318. doi: 10.1128/iai.5.3.311-318.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clausen J. E. Tuberculin-induced migration inhibition of human peripheral leucocytes in agarose medium. Acta Allergol. 1971 Feb;26(1):56–80. doi: 10.1111/j.1398-9995.1971.tb01399.x. [DOI] [PubMed] [Google Scholar]
  7. Collins F. M., Mackaness G. B. Delayed hypersensitivity and arthus reactivity in relation to host resistance in salmonella-infected mice. J Immunol. 1968 Nov;101(5):830–845. [PubMed] [Google Scholar]
  8. DAVID J. R., AL-ASKARI S., LAWRENCE H. S., THOMAS L. DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS. J Immunol. 1964 Aug;93:264–273. [PubMed] [Google Scholar]
  9. Dannenberg A. M., Jr Cellular hypersensitivity and cellular immunity in the pathogensis of tuberculosis: specificity, systemic and local nature, and associated macrophage enzymes. Bacteriol Rev. 1968 Jun;32(2):85–102. doi: 10.1128/br.32.2.85-102.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dumonde D. C., Page D. A., Matthew M., Wolstencroft R. A. Role of lymphocyte activation products (LAP) in cell-mediated immunity. I. Preparation and partial purification of guinea-pig LAP. Clin Exp Immunol. 1972 Jan;10(1):25–47. [PMC free article] [PubMed] [Google Scholar]
  11. Dumonde D. C., Wolstencroft R. A., Panayi G. S., Matthew M., Morley J., Howson W. T. "Lymphokines": non-antibody mediators of cellular immunity generated by lymphocyte activation. Nature. 1969 Oct 4;224(5214):38–42. doi: 10.1038/224038a0. [DOI] [PubMed] [Google Scholar]
  12. Federlin K., Maini R. N., Russell A. S., Dumonde D. C. A micro-method for peripheral leucocyte migration in tuberculin sensitivity. J Clin Pathol. 1971 Sep;24(6):533–536. doi: 10.1136/jcp.24.6.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. GEORGE M., VAUGHAN J. H. In vitro cell migration as a model for delayed hypersensitivity. Proc Soc Exp Biol Med. 1962 Nov;111:514–521. doi: 10.3181/00379727-111-27841. [DOI] [PubMed] [Google Scholar]
  14. Gaines J. D., Araujo F. G., Krahenbuhl J. L., Remington J. S. Simplified in vitro method for measuring delayed hypersensitivity to latent intracellular infection in man (toxoplasmosis). J Immunol. 1972 Jul;109(1):179–182. [PubMed] [Google Scholar]
  15. Goetzl E. J., Austen K. F. A neutrophil-immobilizing factor derived from human leukocytes. I. Generation and partial characterization. J Exp Med. 1972 Dec 1;136(6):1564–1580. doi: 10.1084/jem.136.6.1564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kaltreider H. B., Soghor D., Taylor J. B., Decker J. L. Capillary tube migration for detection of human delayed hypersensitivity: difficulties encountered with "buffy coat" cells and tuberculin antigen. J Immunol. 1969 Aug;103(2):179–184. [PubMed] [Google Scholar]
  17. MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MACKANESS G. B. THE IMMUNOLOGICAL BASIS OF ACQUIRED CELLULAR RESISTANCE. J Exp Med. 1964 Jul 1;120:105–120. doi: 10.1084/jem.120.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Minden P., McClatchy J. K., Farr R. S. Shared antigens between heterologous bacterial species. Infect Immun. 1972 Oct;6(4):574–582. doi: 10.1128/iai.6.4.574-582.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nathan C. F., Remold H. G., David J. R. Characterization of a lymphocyte factor which alters macrophage functions. J Exp Med. 1973 Feb 1;137(2):275–290. doi: 10.1084/jem.137.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nilsson B. S. The response of lymphocytes from tuberculin-positive or negative humans to various doses of PPD-tuberculin in vitro. Cell Immunol. 1972 Mar;3(3):493–500. doi: 10.1016/0008-8749(72)90254-7. [DOI] [PubMed] [Google Scholar]
  22. Park B. H., Fikrig S. M., Smithwick E. M. Infection and nitroblue-tetrazolium reduction by neutrophils. A diagnostic acid. Lancet. 1968 Sep 7;2(7567):532–534. doi: 10.1016/s0140-6736(68)92406-9. [DOI] [PubMed] [Google Scholar]
  23. Rocklin R. E., Meyers O. L., David J. R. An in vitro assay for cellular hypersensitivity in man. J Immunol. 1970 Jan;104(1):95–102. [PubMed] [Google Scholar]
  24. Scheetz M. E., 2nd, Rossio J. L., Dodd M. C. A sensitive and reproducible assay for the quantitation of the MIF correlate of delayed hypersensitivity in man. Immunol Commun. 1972;1(3):211–222. doi: 10.3109/08820137209022936. [DOI] [PubMed] [Google Scholar]
  25. Simon H. B., Sheagren J. N. Enhancement of macrophage bactericidal capacity by antigenically stimulated immune lymphocytes. Cell Immunol. 1972 Jun;4(2):163–174. doi: 10.1016/0008-8749(72)90015-9. [DOI] [PubMed] [Google Scholar]
  26. Simon H. B., Sheagren J. N. Migration inhibitory factor and macrophage bactericidal function. Infect Immun. 1972 Aug;6(2):101–103. doi: 10.1128/iai.6.2.101-103.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stites D. P., Carr M. C., Fudenberg H. H. Development of cellular immunity in the human fetus: dichotomy of proliferative and cytotoxic responses of lymphoid cells to phytohemagglutinin. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1440–1444. doi: 10.1073/pnas.69.6.1440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sultzer B. M., Nilsson B. S. PPD tuberculin--a B-cell mitogen. Nat New Biol. 1972 Dec 13;240(102):198–200. doi: 10.1038/newbio240198a0. [DOI] [PubMed] [Google Scholar]
  29. Thor D. E., Jureziz R. E., Veach S. R., Miller E., Dray S. Cell migration inhibition factor released by antigen from human peripheral lymphocytes. Nature. 1968 Aug 17;219(5155):755–757. doi: 10.1038/219755a0. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES