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. 1973 Sep;8(3):388–394. doi: 10.1128/iai.8.3.388-394.1973

Antimycobacterial Effect of Lysates Prepared from Immunologically Activated Macrophages

Ivan Kochan 1, Carole A Golden 1
PMCID: PMC422860  PMID: 4199717

Abstract

Lysates or heptane extracts of peritoneal (P) and alveolar (A) normal macrophages (N-M), immune macrophages (I-M), and immune-activated macrophages (IA-M) were examined for antimycobacterial activity by the agar-plate diffusion test. This test has been found suitable to reveal the antibacterial activity in 3-day incubated, but not in freshly prepared, lysates. Results showed that materials of IA-AM or I-AM and of IA-PM exerted antimycobacterial effects, whereas materials of N-PM, I-PM, and of N-AM were usually inactive. Antimycobacterial activity of lysates of AM was stronger than that of PM. The formation of antibacterial factors during an incubation of M lysates, the solubility of the factors in heptane, and various other characteristics suggested that the antimycobacterial effect was caused by the formation of toxic levels of non-esterified fatty acids. M lysates exerted equal activities against BCG, H37Ra, and H37Rv strains of tubercle bacilli. The presence of antimycobacterial activity in lysates prepared from IA-M of either BCG- or BCG-sensitized animals indicated that the potential to generate antimycobacterial activity is associated with the state of delayed hypersensitivity and the state of activation of M.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blanden R. V., Lefford M. J., Mackaness G. B. The host response to Calmette-Guérin bacillus infection in mice. J Exp Med. 1969 May 1;129(5):1079–1107. doi: 10.1084/jem.129.5.1079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dannenberg A. M., Jr, Ando M., Shima K. Macrophage accumulation, division, maturation, and digestive and microbicidal capacities in tuberculous lesions. 3. The turnover of macrophages and its relation to their activation and antimicrobial immunity in primary BCG lesions and those of reinfection. J Immunol. 1972 Nov;109(5):1109–1121. [PubMed] [Google Scholar]
  3. Dodd R. Y. Cellular Immunity to Listeria monocytogenes Induced by Sensitization and Challenge with Bovine Gamma Globulin. Infect Immun. 1970 May;1(5):511–512. doi: 10.1128/iai.1.5.511-512.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EISLER D. M., VONMETZ E. ANTI-PASTEURELLA PESTIS FACTOR IN THE ORGANS OF NORMAL MICE AND GUINEA PIGS. I. BIOLOGIC CHARACTERISTICS. J Immunol. 1963 Aug;91:287–294. [PubMed] [Google Scholar]
  5. Eisler D. M., Heckly R. J. Possible mechanisms of action of an anti-Pasteurella pestis factor. J Bacteriol. 1968 Dec;96(6):1977–1981. doi: 10.1128/jb.96.6.1977-1981.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goihman-Yahr M. Raffel S, Ferraresi RW: Delayed hypersensitivity in relation to suppression of growth of Listeria monocytogenes by guinea pig macrophages. J Bacteriol. 1969 Nov;100(2):635–640. doi: 10.1128/jb.100.2.635-640.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KOCHAN I., SMITH L. ANTIMYCOBACTERIAL ACTIVITY OF TUBERCULOSTATIC FACTOR ON INTRACELLULAR BACILLI. J Immunol. 1965 Feb;94:220–227. [PubMed] [Google Scholar]
  8. Kanai K., Kondo E. A suggested role of the lysosomal membrane as a part of the defence mechanism against tuberculous infection. Jpn J Med Sci Biol. 1970 Oct;23(5):295–302. doi: 10.7883/yoken1952.23.295. [DOI] [PubMed] [Google Scholar]
  9. Karnovsky M. L., Shafer A. W., Cagan R. H., Graham R. C., Karnovsky M. J., Glass E. A., Saito K. Membrane function and metabolism in phagocytic cells. Trans N Y Acad Sci. 1966 Apr;28(6):778–787. doi: 10.1111/j.2164-0947.1966.tb03542.x. [DOI] [PubMed] [Google Scholar]
  10. Kochan I., Bendel W. L., Jr Passive transfer of tuberculin hypersensitivity in guinea pigs. J Allergy. 1966 May;37(5):284–294. doi: 10.1016/s0021-8707(96)90012-x. [DOI] [PubMed] [Google Scholar]
  11. Kochan I., Pellis N. R., Pfohl D. G. Effects of normal and activated cell fractions on the growth of tubercle bacilli. Infect Immun. 1972 Aug;6(2):142–148. doi: 10.1128/iai.6.2.142-148.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kochan I. The role of iron in bacterial infections, with special consideration of host-tubercle bacillus interaction. Curr Top Microbiol Immunol. 1973;60:1–30. doi: 10.1007/978-3-642-65502-9_1. [DOI] [PubMed] [Google Scholar]
  13. MYRVIK Q. N., LEAKE E. S., FARISS B. Lysozyme content of alveolar and peritoneal macrophages from the rabbit. J Immunol. 1961 Feb;86:133–136. [PubMed] [Google Scholar]
  14. MYRVIK Q., LEAKE E. S., FARISS B. Studies on pulmonary alveolar macrophages from the normal rabbit: a technique to procure them in a high state of purity. J Immunol. 1961 Feb;86:128–132. [PubMed] [Google Scholar]
  15. Mackaness G. B. The immunology of antituberculous immunity. Am Rev Respir Dis. 1968 Mar;97(3):337–344. doi: 10.1164/arrd.1968.97.3.337. [DOI] [PubMed] [Google Scholar]
  16. North R. J. The mitotic potential of fixed phagocytes in the liver as revealed during the development of cellular immunity. J Exp Med. 1969 Aug 1;130(2):315–326. doi: 10.1084/jem.130.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. OREN R., FARNHAM A. E., SAITO K., MILOFSKY E., KARNOVSKY M. L. Metabolic patterns in three types of phagocytizing cells. J Cell Biol. 1963 Jun;17:487–501. doi: 10.1083/jcb.17.3.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. OSHIMA S., MYRVIK Q. N., LEAKE E. The demonstration of lysozyme as a dominant tuberculostatic factor in extracts of granulomatous lungs. Br J Exp Pathol. 1961 Apr;42:138–144. [PMC free article] [PubMed] [Google Scholar]
  19. Patterson R. J., Youmans G. P. Demonstration in tissue culture of lymphocyte-mediated immunity to tuberculosis. Infect Immun. 1970 Jun;1(6):600–603. doi: 10.1128/iai.1.6.600-603.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Patterson R. J., Youmans G. P. Multiplication of Mycobacterium tuberculosis Within Normal and "Immune" Mouse Macrophages Cultivated With and Without Streptomycin. Infect Immun. 1970 Jan;1(1):30–40. doi: 10.1128/iai.1.1.30-40.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sorber W. A., Leake E. S., Myrvik Q. N. Comparative densities of hydrolase-containing granules from normal and BCG-induced alveolar macrophages. Infect Immun. 1973 Jan;7(1):86–92. doi: 10.1128/iai.7.1.86-92.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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