Table 5.
Differentially expressed genes between untreated and 5-aza treated mycelia
| Gene* | Description | RPKM No aza | RPKM aza | Fold Change | adj. p value |
|---|---|---|---|---|---|
|
Oxidation-reduction process
| |||||
| GSTUMT00004482001 |
Flavin-binding monooxygenase-like protein |
8 |
258 |
33.80 |
3.51E-09 |
| GSTUMT00009102001 |
Extracellular dioxygenase |
18 |
196 |
10.71 |
1.33E-09 |
| GSTUMT00002834001 |
Alpha-ketoglutarate-dependent taurine dioxygenase |
47 |
414 |
8.84 |
4.30E-09 |
| GSTUMT00006862001 |
Alcohol dehydrogenase 1 |
4355 |
34021 |
7.81 |
4.81E-08 |
| GSTUMT00005793001 |
Bilirubin oxidase |
1590 |
8041 |
5.06 |
2.28E-05 |
| GSTUMT00000137001 |
Cytochrome P450 |
3 |
17 |
5.06 |
5.85E-04 |
| GSTUMT00006911001 |
Multicopper oxidase |
19 |
90 |
4.64 |
4.87E-03 |
| GSTUMT00008228001 |
Zinc-binding oxidoreductase |
34 |
147 |
4.35 |
3.20E-04 |
| GSTUMT00002706001 |
Alpha-ketoglutarate-dependent sulfonate dioxygenase protein |
4 |
14 |
3.61 |
3.51E-02 |
| GSTUMT00000322001 |
Fatty acid oxygenase |
137 |
48 |
0.35 |
4.81E-02 |
| GSTUMT00000528001 |
Short-chain alcohol dehydrogenases |
334 |
110 |
0.33 |
1.22E-02 |
| GSTUMT00007846001 |
FAD binding domain-containing protein |
172 |
56 |
0.33 |
1.54E-02 |
| GSTUMT00002653001 |
Pyridoxal reductase |
104 |
31 |
0.30 |
5.83E-03 |
| GSTUMT00000530001 |
FAD binding domain-containing protein |
61 |
18 |
0.30 |
8.39E-03 |
| GSTUMT00012134001 |
Dopa -dioxygenase |
4704 |
1368 |
0.29 |
3.10E-03 |
| GSTUMT00000158001 |
Ferritin ribonucleotide reductase-like protein |
54 |
15 |
0.28 |
4.52E-03 |
| GSTUMT00002858001 |
3-oxoacyl-(acyl-carrier-protein) reductase |
22 |
5 |
0.25 |
8.53E-03 |
| GSTUMT00012200001 |
Malate synthase |
71 |
15 |
0.21 |
2.41E-03 |
| GSTUMT00001645001 |
Zinc-binding alcohol |
219 |
34 |
0.16 |
1.06E-06 |
| GSTUMT00006980001 |
Alcohol dehydrogenase |
1022 |
146 |
0.14 |
1.36E-07 |
| GSTUMT00007117001 |
D-isomer specific 2-hydroxyacid dehydrogenase |
4 |
0.4 |
0.10 |
4.66E-02 |
|
Transmembrane transport
| |||||
| GSTUMT00001488001 |
Amino acid permease |
163 |
737 |
4.53 |
9.07E-03 |
| GSTUMT00003668001 |
Carboxylic acid transport protein |
20 |
82 |
4.03 |
6.04E-03 |
| GSTUMT00010777001 |
Mate efflux family protein |
16 |
66 |
4.03 |
1.22E-02 |
| GSTUMT00008972001 |
Lactose permease |
16 |
64 |
4.02 |
7.12E-03 |
| GSTUMT00001400001 |
MFS efflux transporter |
86 |
295 |
3.44 |
5.58E-03 |
| GSTUMT00000284001 |
Mg2+ transporter family-like protein |
1082 |
366 |
0.34 |
2.56E-02 |
| GSTUMT00008966001 |
MFS general substrate transporter |
27 |
9 |
0.34 |
4.85E-02 |
| GSTUMT00000070001 |
MFS multidrug transporter |
119 |
30 |
0.25 |
1.20E-03 |
| GSTUMT00004586001 |
ABC transporter |
32 |
5 |
0.17 |
3.72E-02 |
| GSTUMT00005001001 |
MFS drug efflux |
663 |
84 |
0.13 |
4.47E-08 |
|
Transcription factors
| |||||
| GSTUMT00005836001 |
Fungal transcriptional regulatory protein |
1 |
18 |
17.23 |
1.91E-09 |
| GSTUMT00010369001 |
STE12-like transcription factor |
1 |
7 |
12.50 |
1.93E-04 |
| GSTUMT00004615001 |
Binuclear zinc transcription factor |
2 |
10 |
6.40 |
1.95E-04 |
| GSTUMT00004613001 |
GAL4 domain-containing protein |
90 |
402 |
4.49 |
1.03E-02 |
| GSTUMT00003504001 |
C2H2 finger domain |
1522 |
467 |
0.31 |
4.98E-03 |
|
Metabolic process
| |||||
| GSTUMT00008986001 |
Glycoside hydrolase family 61 protein |
0.2 |
5 |
25.60 |
1.10E-04 |
| GSTUMT00001850001 |
Endo- -beta-glucanase eng1 |
94 |
1499 |
16.02 |
4.71E-13 |
| GSTUMT00012011001 |
Glycoside hydrolase family 28 protein |
7 |
57 |
7.73 |
3.97E-04 |
| GSTUMT00005539001 |
Dynamin family protein |
27 |
195 |
7.18 |
2.59E-05 |
| GSTUMT00010778001 |
Agmatinase 1 |
230 |
1316 |
5.72 |
1.70E-05 |
| GSTUMT00011981001 |
Acetyltransferase |
298 |
1376 |
4.62 |
1.62E-04 |
| GSTUMT00007852001 |
Alpha amylase |
166 |
704 |
4.25 |
6.60E-04 |
| GSTUMT00003032001 |
Polysaccharide deacetylase |
1222 |
4444 |
3.64 |
3.18E-03 |
| GSTUMT00001583001 |
Pirin |
273 |
968 |
3.54 |
3.99E-03 |
| GSTUMT00005439001 |
Glycerophosphoryl diester phosphodiesterase |
214 |
758 |
3.54 |
1.08E-02 |
| GSTUMT00001660001 |
Glycoside hydrolase family 16 protein |
109 |
374 |
3.43 |
6.66E-03 |
| GSTUMT00006417001 |
Fatty acid activator |
417 |
1410 |
3.38 |
5.16E-03 |
| GSTUMT00007213001 |
Tat pathway signal sequence |
74 |
239 |
3.25 |
1.98E-02 |
| GSTUMT00002512001 |
Gnat family N-acetyltransferase |
99 |
311 |
3.14 |
2.83E-02 |
| GSTUMT00000969001 |
Branched-chain amino acid aminotransferase |
840 |
270 |
0.32 |
9.18E-03 |
|
Response to stimuli/mycelium development
| |||||
| GSTUMT00004040001 |
Regulator of G protein signaling domain protein |
54 |
209 |
3.84 |
1.40E-03 |
| GSTUMT00007138001 |
Osmolarity two-component sensor histidine kinase SLN1 |
401 |
1134 |
2.83 |
4.81E-02 |
| GSTUMT00004012001 |
Annexin |
72 |
23 |
0.33 |
1.38E-02 |
|
Others
| |||||
| GSTUMT00004681001 |
RNase P RPR2 RPP21 subunit domain-containing protein |
52 |
339 |
6.53 |
9.70E-07 |
| GSTUMT00002939001 |
Allergenic cerato-platanin ASP F13 |
361 |
1730 |
4.80 |
5.46E-05 |
| GSTUMT00010365001 |
Putative endo-glucanase |
8 |
39 |
4.66 |
1.93E-02 |
| GSTUMT00006611001 |
Protoglobin protein |
999 |
3827 |
3.83 |
3.11E-03 |
| GSTUMT00006718001 |
Glutathione S-transferase |
228 |
80 |
0.35 |
3.10E-02 |
| GSTUMT00008071001 |
XAA-pro dipeptidase |
1272 |
413 |
0.32 |
1.27E-02 |
| GSTUMT00001676001 |
Arrestin (or S-antigen) N-terminal domain protein |
96 |
31 |
0.32 |
1.22E-02 |
| GSTUMT00006632001 | P-loop containing nucleoside triphosphate hydrolase | 3053 | 724 | 0.24 | 2.20E-04 |
*One hundred fifteen genes undergo a significant change in expression levels (adjusted p value <0.05) upon 5-aza treatment. Functional annotation of the differentially expressed genes/sequences (either up- or down-regulated) was performed with the Blast2GO suite. Fifty-three genes code for hypothetical proteins. The remaining 62 genes were classified into distinct functional categories. “Oxidation-reduction process” and “transmembrane transport” appear as enriched GO terms (Fisher exact test p = 2.6E-4 and p = 7.9E-3, respectively) among differentially expressed genes.