Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1973 Oct;8(4):650–656. doi: 10.1128/iai.8.4.650-656.1973

Immunity Against Parainfluenza-3 Virus in Cattle: Anti-Neuraminidase Activity in Serum and Nasal Secretion

B Morein 1,2, S Höglund 1,2, R Bergman 1,2
PMCID: PMC422905  PMID: 4355138

Abstract

The antigenicity of two parainfluenza=3 virus strains, a “neuraminidasestrong” and a “neuraminidase-weak,” was compared. For both strains the amount of hemagglutinin units was equal. The antibody responses to neuraminidase and hemagglutinin were measured on samples of serum and nasal secretion and were found to be similar, irrespective of the strain used for immunization. Anti-neuraminidase activity was demonstrated in the gel phase of nasal secretion of immunized cattle. Immunglobulin A was found attached to the peplomers of inhibited virus by immuno-electron microscopy.

Full text

PDF
650

Images in this article

654Image
on p.654
655Image
on p.655

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADA G. L., LIND P. E. AN IMMUNOLOGICAL STUDY OF AVIAN, VIRAL AND BACTERIAL NEURAMINIDASE BASED ON SPECIFIC INHIBITION OF ENZYME BY ANTIBODY. J Gen Microbiol. 1963 Aug;32:225–233. doi: 10.1099/00221287-32-2-225. [DOI] [PubMed] [Google Scholar]
  2. Bloth B., Svehag S. E. Further studies on the ultrastructure of dimeric IgA of human origin. J Exp Med. 1971 May 1;133(5):1035–1042. doi: 10.1084/jem.133.5.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brostrom M. A., Bruening G., Bankowski R. A. Comparison of neuraminidases of paramyxoviruses with immunologically dissimilar hemagglutinins. Virology. 1971 Dec;46(3):856–865. doi: 10.1016/0042-6822(71)90086-9. [DOI] [PubMed] [Google Scholar]
  4. Downie J. C. Neuraminidase- and hemagglutinin-inhibiting antibodies in serum and nasal secretions of volunteers immunized with attenuated and inactivated influenza B-Eng-13-65 virus vaccines. J Immunol. 1970 Sep;105(3):620–626. [PubMed] [Google Scholar]
  5. Drzeniek R., Bögel K., Rott R. On the classification of bovine parainfluenza 3 viruses. Virology. 1967 Apr;31(4):725–727. doi: 10.1016/0042-6822(67)90204-8. [DOI] [PubMed] [Google Scholar]
  6. Drzeniek R. Viral and bacterial neuraminidases. Curr Top Microbiol Immunol. 1972;59:35–74. doi: 10.1007/978-3-642-65444-2_2. [DOI] [PubMed] [Google Scholar]
  7. Fedson D. S., Fulk R. V., Huber M. A., Reisberg M. A., Kasel J. A. Anti-neuraminidase antibody response in serum and nasal secretions following intranasal or subcutaneous inactivated A2-Hong Kong-68 influenza virus vaccine. J Immunol. 1971 Sep;107(3):730–737. [PubMed] [Google Scholar]
  8. Hennessy A. V., Minuse E., Davenport F. M. Antineuraminidase antibody response of man to influenza virus neuraminidase N2: results obtained with an improved hemagglutination inhibition technique and an enzyme inhibition test. J Immunol. 1972 Aug;109(2):213–216. [PubMed] [Google Scholar]
  9. Kilburn K. H. Cilia and mucus transport as determinants of the response of lung to air pollutants. Arch Environ Health. 1967 Jan;14(1):77–91. doi: 10.1080/00039896.1967.10664699. [DOI] [PubMed] [Google Scholar]
  10. Morein B., Bergman R. Effect of parainfluenza-3 neuraminidase on bovine nasal secretion. Infect Immun. 1972 Aug;6(2):174–177. doi: 10.1128/iai.6.2.174-177.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morein B. Immunity against parainfluenza-3 virus in cattle. IgA in nasal secretions. Int Arch Allergy Appl Immunol. 1970;39(4):403–414. doi: 10.1159/000230368. [DOI] [PubMed] [Google Scholar]
  12. Morein B. Immunity against parainfluenza-3 virus in cattle; immunoglobulins in serum and nasal secretions after subcutaneous and nasal vaccination. Z Immunitatsforsch Exp Klin Immunol. 1972 Jul;144(1):63–74. [PubMed] [Google Scholar]
  13. Porter P., Noakes D. E. Immunoglobulin IgA in bovine serum and external secretions. Biochim Biophys Acta. 1970 Jul 27;214(1):107–116. doi: 10.1016/0005-2795(70)90074-7. [DOI] [PubMed] [Google Scholar]
  14. Rylander R. Studies of lung defense to infections in inhalation toxicology. Arch Intern Med. 1970 Sep;126(3):496–499. [PubMed] [Google Scholar]
  15. Scheid A., Caliguiri L. A., Compans R. W., Choppin P. W. Isolation of paramyxovirus glycoproteins. Association of both hemagglutinating and neuraminidase activities with the larger SV5 glycoprotein. Virology. 1972 Dec;50(3):640–652. doi: 10.1016/0042-6822(72)90418-7. [DOI] [PubMed] [Google Scholar]
  16. Scheid A., Choppin P. W. Isolation and purification of the envelope proteins of Newcastle disease virus. J Virol. 1973 Feb;11(2):263–271. doi: 10.1128/jvi.11.2.263-271.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schulman J. L., Kilbourne E. D. Independent variation in nature of hemagglutinin and neuraminidase antigens of influenza virus: distinctiveness of hemagglutinin antigen of Hong Kong-68 virus. Proc Natl Acad Sci U S A. 1969 Jun;63(2):326–333. doi: 10.1073/pnas.63.2.326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stephan W., Róka L. Adsorption von Lipoproteiden. Behandlung von Seren mit Adsorbentien. I. Z Klin Chem Klin Biochem. 1968 May;6(3):186–190. [PubMed] [Google Scholar]
  19. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  20. Williams R. C., Fisher H. W. Electron microscopy of tobacco mosaic virus under conditions of minimal beam exposure. J Mol Biol. 1970 Aug 28;52(1):121–123. doi: 10.1016/0022-2836(70)90181-6. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES