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. 1973 Oct;8(4):657–664. doi: 10.1128/iai.8.4.657-664.1973

Persistent Newcastle Disease Virus Infection in Embryonic Chicken Tracheal Organ Cultures

J F Cummiskey 1, J V Hallum 1, M S Skinner 1, G A Leslie 1
PMCID: PMC422906  PMID: 4355139

Abstract

The persistent infection of embryonic chicken tracheal organ cultures with Newcastle disease virus (NDV) is described. Tracheal explants remained morphologically intact and were able to support the replication of NDV for 6 months. Peak titers of released virus occurred at 1 week postinfection, whereas maximal immunofluorescence was not observed until 30 days postinfection. The inoculum titer was not critical, and viral persistence resulted with either of two strains of NDV tested. Serum was not required in the medium for explant viability or to maintain the persistent infection. The presence of a contaminating virus morphologically resembling a leukovirus neither altered the course of infection nor affected the survivability of explants. Although interferon was not detected in the culture medium, persistently infected explants were resistant to heterologous viral challenge, and a similar resistant state could be induced in uninfected explants with exogenous interferon or ultraviolet light-inactivated NDV. No evidence was found to implicate antibody as a regulatory factor in the establishment or maintenance of persistence. The results from electron microscopy and immunofluorescence suggest the cells of the subepithelial connective tissue as the site of NDV persistence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BANG F. B., FOARD M. INTERACTION OF RESPIRATORY EPITHELIUM OF THE CHICK AND NEWCASTLE DISEASE VIRUS. Am J Hyg. 1964 May;79:260–278. doi: 10.1093/oxfordjournals.aje.a120382. [DOI] [PubMed] [Google Scholar]
  2. Beard C. W., Easterday B. C. The influence of the route of administration of Newcastle disease virus on host response. 3. Immunofluorescent and histopathological studies. J Infect Dis. 1967 Feb;117(1):66–70. doi: 10.1093/infdis/117.1.66. [DOI] [PubMed] [Google Scholar]
  3. Blaskovic P., Rhodes A. J., Doane F. W., Labzoffsky N. A. Infection of chick embryo tracheal organ cultures with influenza A2 (Hong Kong) virus. II. Electron microscopy. Arch Gesamte Virusforsch. 1972;38(2):250–266. doi: 10.1007/BF01249676. [DOI] [PubMed] [Google Scholar]
  4. Blaskovic P., Rhodes A. J., Labzoffsky N. A. Infection of chick embryo tracheal organ cultures with influenza A2 (Hong Kong) virus. I. Cytopathology, histopathology, immunofluorescence, hemadsorption, and titration of the released infectious progeny virus. Arch Gesamte Virusforsch. 1972;37(1):104–113. doi: 10.1007/BF01241156. [DOI] [PubMed] [Google Scholar]
  5. Boquist L. Intranuclear rods in pancreatic islet beta-cells. J Cell Biol. 1969 Nov;43(2):377–381. doi: 10.1083/jcb.43.2.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Butler M., Ellaway W. J., Hall T. Comparative studies on the infectivity of avian respiratory viruses for eggs, cell cultures and tracheal explants. J Comp Pathol. 1972 Jul;82(3):327–332. doi: 10.1016/0021-9975(72)90013-8. [DOI] [PubMed] [Google Scholar]
  7. Cherry J. D., Taylor-Robinson D. Large-quantity production of chicken embryo tracheal organ cultures and use in virus and mycoplasma studies. Appl Microbiol. 1970 Apr;19(4):658–662. doi: 10.1128/am.19.4.658-662.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Clattenburg R. E., Singh R. P., Montemurro D. G. Intranuclear filamentous inclusions in neurons of the rabbit hypothalamus. J Ultrastruct Res. 1972 Jun;39(5):549–555. doi: 10.1016/s0022-5320(72)90120-7. [DOI] [PubMed] [Google Scholar]
  9. Finkelstein M. S., Bausek G. H., Merigan T. C. Interferon inducers in vitro: difference in sensitivity to inhbitiros of RNA and protein synthesis. Science. 1968 Aug 2;161(3840):465–468. doi: 10.1126/science.161.3840.465. [DOI] [PubMed] [Google Scholar]
  10. Heuschele W. P., Easterday B. C. Local immunity and persistence of virus in the tracheas of chickens following infection with Newcastle Disease Virus. II. Immunofluorescent and histopathologic studies. J Infect Dis. 1970 May;121(5):497–504. doi: 10.1093/infdis/121.5.497. [DOI] [PubMed] [Google Scholar]
  11. Heuschele W. P., Easterday B. C. Local immunity and persistence of virus in the tracheas of chickens following infection with Newcastle disease virus. I. Organ culture studies. J Infect Dis. 1970 May;121(5):486–496. doi: 10.1093/infdis/121.5.486. [DOI] [PubMed] [Google Scholar]
  12. Leslie G. A., Martin L. N. Studies on the secretory immunologic system of fowl. 3. Serum and secretory IgA of the chicken. J Immunol. 1973 Jan;110(1):1–9. [PubMed] [Google Scholar]
  13. Oyanagi S., ter Meulen V., Katz M., Koprowski H. Comparison of subacute sclerosing panencephalitis and measles viruses: an electron microscope study. J Virol. 1971 Jan;7(1):176–187. doi: 10.1128/jvi.7.1.176-187.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reed S. E., Boyde A. Organ cultures of respiratory epithelium infected with rhinovirus or parainfluenza virus studied in a scanning electron microscope. Infect Immun. 1972 Jul;6(1):68–76. doi: 10.1128/iai.6.1.68-76.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reed S. E. Persistent respiratory virus infection in tracheal organ cultures. Br J Exp Pathol. 1969 Aug;50(4):378–388. [PMC free article] [PubMed] [Google Scholar]
  16. Seïte R. Etude ultrastructurale de divers types d'inclusions nucléaires dans les neurones sympathiques du chat. J Ultrastruct Res. 1970 Jan;30(1):152–165. doi: 10.1016/s0022-5320(70)90069-9. [DOI] [PubMed] [Google Scholar]
  17. Smorodintsev A. A. The production and effect of interferon in organ cultures of calf trachea. Br J Exp Pathol. 1968 Oct;49(5):511–515. [PMC free article] [PubMed] [Google Scholar]
  18. Thacore H., Youngner J. S. Cells persistently infected with newcastle disease virus: I. Properties of mutants isolated from persistently infected L cells. J Virol. 1969 Sep;4(3):244–251. doi: 10.1128/jvi.4.3.244-251.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WALKER D. L. THE VIRAL CARRIER STATE IN ANIMAL CELL CULTURES. Prog Med Virol. 1964;6:111–148. [PubMed] [Google Scholar]
  20. Willems F. T., van der Veen J. Growth of Sendai virus in organ cultures of mouse tissue. Arch Gesamte Virusforsch. 1968;23(1):148–156. doi: 10.1007/BF01242123. [DOI] [PubMed] [Google Scholar]
  21. Youngner J. S., Scott A. W., Hallum J. V., Stinebring W. R. Interferon production by inactivated Newcastle disease virus in cell cultures and in mice. J Bacteriol. 1966 Oct;92(4):862–868. doi: 10.1128/jb.92.4.862-868.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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