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. 1973 Nov;8(5):775–780. doi: 10.1128/iai.8.5.775-780.1973

Purification and Characterization of Thermostable Direct Hemolysin of Vibrio parahaemolyticus

Jun Sakurai 1, Akiko Matsuzaki 1, Toshio Miwatani 1
PMCID: PMC422926  PMID: 4201322

Abstract

A thermostable direct hemolysin was purified from culture filtrates of Vibrio parahaemolyticus. The purified hemolysin gave one precipitation line with the antihemolysin antiserum on agar-gel diffusion test and a single band on polyacrylamide gel electrophoresis. The hemolysin was not inactivated by heating at 70 to 100 C for 10 min. The hemolytic activity was not enhanced by the addition of lecithin. It was demonstrated that the hemolysin was a protein with a molecular weight of approximately 118,000. Amino acid analysis revealed that 43% of total amino acids were acidic amino acids, whereas 11% were basic amino acids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALOUF J. E., RAYNAUD M. Purification of streptolysin O. Nature. 1962 Oct 27;196:374–375. doi: 10.1038/196374a0. [DOI] [PubMed] [Google Scholar]
  2. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bernheimer A. W. Physical behavior of streptolysin S. J Bacteriol. 1967 Jun;93(6):2024–2025. doi: 10.1128/jb.93.6.2024-2025.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CHESBRO W. R., HEYDRICK F. P., MARTINEAU R., PERKINS G. N. PURIFICATION OF STAPHYLOCOCCAL BETA-HEMOLYSIN AND ITS ACTION ON STAPHYLOCOCCAL AND STREPTOCOCCAL CELL WALLS. J Bacteriol. 1965 Feb;89:378–389. doi: 10.1128/jb.89.2.378-389.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  6. Fujino T., Miwatani T., Takeda Y., Tomaru A. A thermolabile direct hemolysin of Vibrio parahaemolyticus. Biken J. 1969 Jun;12(2):145–148. [PubMed] [Google Scholar]
  7. Kantor H. S., Temples B., Shaw W. V. Staphylococcal delta hemolysin: purification and characterization. Arch Biochem Biophys. 1972 Jul;151(1):142–156. doi: 10.1016/0003-9861(72)90483-3. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Miwatani T., Sakurai J., Yoshihara A., Takeda Y. Isolation and partial purification of thermolabile direct hemolysin of Vibrio parahaemolyticus. Biken J. 1972 Jun;15(2):61–66. [PubMed] [Google Scholar]
  10. Miwatani T., Takeda Y., Sakurai J., Yoshihara A., Taga S. Effect of heat (Arrhenius effect) on crude hemolysin of Vibrio parahaemolyticus. Infect Immun. 1972 Dec;6(6):1031–1033. doi: 10.1128/iai.6.6.1031-1033.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Miwtani T., Shinoda S., Nishimune H., Okada M., Takeda Y. A common antigenic substance of Vibrio parahaemolyticus. I. Isolation and purification. Biken J. 1969 Jun;12(2):97–106. [PubMed] [Google Scholar]
  12. Miyamoto Y., Kato T., Obara Y., Akiyama S., Takizawa K., Yamai S. In vitro hemolytic characteristic of Vibrio parahaemolyticus: its close correlation with human pathogenicity. J Bacteriol. 1969 Nov;100(2):1147–1149. doi: 10.1128/jb.100.2.1147-1149.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morris D. L. Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent. Science. 1948 Mar 5;107(2775):254–255. doi: 10.1126/science.107.2775.254. [DOI] [PubMed] [Google Scholar]
  14. Yanagase Y., Inoue K., Ozaki M., Ochi T., Amano T. Hemolysins and related enzymes of Vibrio parahaemolyticus. I. Identification and partial purification of enzymes. Biken J. 1970 Jun;13(2):77–92. [PubMed] [Google Scholar]
  15. Zen-Yoji H., Hitokoto H., Morozumi S., Le Clair R. A. Purification and characterization o;f a hemolysin produced by Vibrio parahaemolyticus. J Infect Dis. 1971 Jun;123(6):665–667. doi: 10.1093/infdis/123.6.665. [DOI] [PubMed] [Google Scholar]

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