Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1973 Nov;8(5):804–813. doi: 10.1128/iai.8.5.804-813.1973

Isolation of a New Simian Foamy Virus from a Spider Monkey Brain Culture

John J Hooks 1,2,3, C J Gibbs Jr 1,2,3, S Chou 1,2,3, R Howk 1,2,3, M Lewis 1,2,3, D C Gajdusek 1,2,3
PMCID: PMC422931  PMID: 4201323

Abstract

A syncytium-forming (foamy) virus was isolated from a spider monkey brain cell culture. Cytopathic effect was observed both in the brain culture and in human embryonic kidney cells. Neutralizing antibody was present in the sera of the spider monkey from whom the isolation was made. The virus was inhibited by 5-bromo-2-deoxyuridine (20 μg/ml), contained a ribonucleic acid-dependent deoxyribonucleic acid polymerase, and had an infectivity peak at 1.15 g/cm3 in a sucrose density gradient. The virus passed through a 220-nm but not a 100-nm membrane filter, was chloroform sensitive, and was inactivated at 56 C in 30 min. Hemagglutinating and hemadsorption activity was not noted with a variety of erythrocytes. The virion was spherical, formed in the cytoplasm, and was 105 to 115 nm in diameter. Ring-shaped nucleoids, 45 to 50 nm in diameter, were associated with tubular profiles. The virus was not neutralized by sera prepared against known viruses, including simian foamy virus types 1 through 7, Mason-Pfizer monkey virus, and bovine syncytial and measles viruses. Sera from a rabbit hyperimmunized with the isolate and sera from 19 spider monkeys had neutralizing antibody to the isolate; however, these sera did not cross-react with simian foamy virus types 1 through 7. Neutralizing antibody to the isolate was not detected in sera from 16 humans, 9 rhesus monkeys, and 10 chimpanzees.

Full text

PDF
804

Images in this article

808Image
on p.808
809Image
on p.809
810Image
on p.810
811Image
on p.811

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achong B. G., Mansell P. W., Epstein M. A., Clifford P. An unusual virus in cultures from a human nasopharyngeal carcinoma. J Natl Cancer Inst. 1971 Feb;46(2):299–307. [PubMed] [Google Scholar]
  2. Boothe A. D., Van der Maaten M. J., Malmquist W. A. Morphological variation of a syncytial virus from lymphosarcomatous and apparently normal cattle. Arch Gesamte Virusforsch. 1970;31(3):373–384. doi: 10.1007/BF01253771. [DOI] [PubMed] [Google Scholar]
  3. Chopra H. C., Hooks J. J., Walling M. J., Gibbs C. J., Jr Morphology of simian foamy viruses, with particular reference to virus isolated from spontaneous tumor of a rhesus monkey. J Natl Cancer Inst. 1972 Feb;48(2):451–463. [PubMed] [Google Scholar]
  4. Gajdusek D. C., Rogers N. G., Basnight M., Gibbs C. J., Jr, Alpers M. Transmission experiments with kuru in chimpanzees and the isolation of latent viruses from the explanted tissues of affected animals. Ann N Y Acad Sci. 1969 Jul 3;162(1):529–550. doi: 10.1111/j.1749-6632.1969.tb56401.x. [DOI] [PubMed] [Google Scholar]
  5. Hooks J. J., Gibbs C. J., Jr, Cutchins E. C., Rogers N. G., Lampert P., Gajdusek D. C. Characteization and distribution of two new foamy viruses isolated from chimpanzees. Arch Gesamte Virusforsch. 1972;38(1):38–55. doi: 10.1007/BF01241354. [DOI] [PubMed] [Google Scholar]
  6. JOHNSTON P. B. A second immunologic type of simian foamy virus: monkey throat infections and unmasking by both types. J Infect Dis. 1961 Jul-Aug;109:1–9. doi: 10.1093/infdis/109.1.1. [DOI] [PubMed] [Google Scholar]
  7. Johnston P. B. Taxonomic features of seven serotypes of simian and ape foamy viruses. Infect Immun. 1971 Jun;3(6):793–799. doi: 10.1128/iai.3.6.793-799.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Malmquist W. A., Van der Maaten M. J., Boothe A. D. Isolation, immunodiffusion, immunofluorescence, and electron microscopy of a syncytial virus of lymphosarcomatous and apparently normal cattle. Cancer Res. 1969 Jan;29(1):188–200. [PubMed] [Google Scholar]
  9. McKissick G. E., Lamont P. H. Characteristics of a virus isolated from a feline fibrosarcoma. J Virol. 1970 Feb;5(2):247–257. doi: 10.1128/jvi.5.2.247-257.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Melendez L. V., Hunt R. D., Daniel M. D., Blake B. J., Garcia F. G. Acute lymphocytic leukemia in owl monkeys inoculated with herpesvirus saimiri. Science. 1971 Mar 19;171(3976):1161–1163. doi: 10.1126/science.171.3976.1161. [DOI] [PubMed] [Google Scholar]
  11. Melendez L. V., Hunt R. D., King N. W., Barahona H. H., Daniel M. D., Fraser C. E., Garcia F. G. Herpesvirus ateles, a new lymphoma virus of monkeys. Nat New Biol. 1972 Feb 9;235(58):182–184. doi: 10.1038/newbio235182b0. [DOI] [PubMed] [Google Scholar]
  12. Parks W. P., Todaro G. J. Biological properties of syncytium-forming ("foamy") viruses. Virology. 1972 Mar;47(3):673–683. doi: 10.1016/0042-6822(72)90557-0. [DOI] [PubMed] [Google Scholar]
  13. Parks W. P., Todaro G. J., Scolnick E. M., Aaronson S. A. RNA dependent DNA polymerase in primate syncytium-forming (foamy) viruses. Nature. 1971 Jan 22;229(5282):258–260. doi: 10.1038/229258a0. [DOI] [PubMed] [Google Scholar]
  14. RUSTIGIAN R., JOHNSTON P., REIHART H. Infection of monkey kidney tissue cultures with virus-like agents. Proc Soc Exp Biol Med. 1955 Jan;88(1):8–16. doi: 10.3181/00379727-88-21478. [DOI] [PubMed] [Google Scholar]
  15. Rogers N. G., Basnight M., Gibbs C. J., Gajdusek D. C. Latent viruses in chimpanzees with experimental kuru. Nature. 1967 Nov 4;216(5114):446–449. doi: 10.1038/216446a0. [DOI] [PubMed] [Google Scholar]
  16. Ross J., Scolnick E. M., Todaro G. J., Aaronson S. A. Separation of murine cellular and murine leukaemia virus DNA polymerases. Nat New Biol. 1971 Jun 9;231(23):163–167. doi: 10.1038/newbio231163a0. [DOI] [PubMed] [Google Scholar]
  17. STILES G. E., BITTLE J. L., CABASSO V. J. COMPARISON OF SIMIAN FOAMY VIRUS STRAINS INCLUDING A NEW SEROLOGICAL TYPE. Nature. 1964 Mar 28;201:1350–1351. doi: 10.1038/2011350a0. [DOI] [PubMed] [Google Scholar]
  18. Scolnick E. M., Parks W. P., Todaro G. J., Aaronson S. A. Immunological characterization of primate C-type virus reverse transcriptases. Nat New Biol. 1972 Jan 12;235(54):35–40. doi: 10.1038/newbio235035a0. [DOI] [PubMed] [Google Scholar]
  19. VOGEL J., SHELOKOV A. Adsorption-hemagglutination test for influenza virus in monkey kidney tissue culture. Science. 1957 Aug 23;126(3269):358–359. doi: 10.1126/science.126.3269.358-a. [DOI] [PubMed] [Google Scholar]
  20. Valerio M., Landon J. C., Innes J. R. Neoplastic diseases in simians. J Natl Cancer Inst. 1968 Apr;40(4):751–756. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES