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. 1973 Dec;8(6):901–906. doi: 10.1128/iai.8.6.901-906.1973

Immunological Studies on the Envelope Component of Venezuelan Equine Encephalomyelitis Virus

Carl E Pedersen Jr 1, Donald R Slocum 1, Gerald A Eddy 1
PMCID: PMC422948  PMID: 4206344

Abstract

Treatment of purified Venezuelan equine encephalomyelitis virus with the nonionic detergent Triton X-100 permitted spearation of the envelope from the core component. The isolated envelope was a noninfectious immunogen which reacted in hemagglutination, hemagglutination inhibition, complement fixation, and neutralization serological reactions.

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Selected References

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  1. Acheson N. H., Tamm I. Replication of Semliki Forest virus: an electron microscopic study. Virology. 1967 May;32(1):128–143. doi: 10.1016/0042-6822(67)90261-9. [DOI] [PubMed] [Google Scholar]
  2. Appleyard G., Oram J. D., Stanley J. L. Dissociation of Semliki Forest virus into biologically active components. J Gen Virol. 1970 Dec;9(3):179–189. doi: 10.1099/0022-1317-9-3-179. [DOI] [PubMed] [Google Scholar]
  3. Bose H. R., Sagik B. P. Immunological activity associated with the nucleocapsid and envelope components of an arbovirus. J Virol. 1970 Mar;5(3):410–412. doi: 10.1128/jvi.5.3.410-412.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
  5. CROWLE A. J. A simplified micro double-diffusion agar precipitin technique. J Lab Clin Med. 1958 Nov;52(5):784–787. [PubMed] [Google Scholar]
  6. Cartwright B., Smale C. J., Brown F. Dissection of vesicular stomatitis virus into the infective ribonucleoprotein and immunizing components. J Gen Virol. 1970 Apr;7(1):19–32. doi: 10.1099/0022-1317-7-1-19. [DOI] [PubMed] [Google Scholar]
  7. Cartwright B., Talbot P., Brown F. The proteins of biologically active sub-units of vesicular stomatitis virus. J Gen Virol. 1970 Jun;7(3):267–272. doi: 10.1099/0022-1317-7-3-267. [DOI] [PubMed] [Google Scholar]
  8. Dobos P., Faulkner P. On the infectivity of the Sindbis virus nucleocapsid. Can J Microbiol. 1970 Dec;16(12):1273–1283. doi: 10.1139/m70-213. [DOI] [PubMed] [Google Scholar]
  9. Faulkner P., Dobos P. Investigations on the formation and interconversion of Sindbis virus hemagglutinins. Can J Microbiol. 1968 Jan;14(1):45–51. doi: 10.1139/m68-008. [DOI] [PubMed] [Google Scholar]
  10. Friedman R. M. Structural and nonstructural proteins of an arbovirus. J Virol. 1968 Oct;2(10):1076–1080. doi: 10.1128/jvi.2.10.1076-1080.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. György E., Sheehan M. C., Sokol F. Release of envelope glycoprotein from rabies virions by a nonionic detergent. J Virol. 1971 Nov;8(5):649–655. doi: 10.1128/jvi.8.5.649-655.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hay A. J., Skehel J. J., Burke D. C. Proteins synthesized in chick cells following infection with Semliki Forest virus. J Gen Virol. 1968 Sep;3(2):175–184. doi: 10.1099/0022-1317-3-2-175. [DOI] [PubMed] [Google Scholar]
  13. Horzinek M., Mussgay M. Studies on the nucleocapsid structure of a group A arbovirus. J Virol. 1969 Oct;4(4):514–520. doi: 10.1128/jvi.4.4.514-520.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Igarashi A., Fukuoka T., Fukai K. Passive immunization of mice with rabbit antisera against Chikungunya virus and its components. Biken J. 1971 Sep;14(3):353–355. [PubMed] [Google Scholar]
  15. Igarashi A., Fukuoka T., Nithiuthai P., Hsu L. C., Fukai K. Structural components of chikungunya virus. Biken J. 1970 Jun;13(2):93–110. [PubMed] [Google Scholar]
  16. Igarashi A., Nithiuthai P., Rojanasuphot S. Immunological properties of Chikungunya virus and its components. Biken J. 1970 Sep;13(3):229–231. [PubMed] [Google Scholar]
  17. Kang C. Y., Prevec L. Proteins of vesicular stomatitis virus. II. Immunological comparisons of viral antigens. J Virol. 1970 Jul;6(1):20–27. doi: 10.1128/jvi.6.1.20-27.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  20. Levitt N. H., Amsler K. R., McKinney R. W. Rapid detection of viral antibody by cellulose acetate electrophoresis. Appl Microbiol. 1971 Jul;22(1):143–144. doi: 10.1128/am.22.1.143-144.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. MORGAN C., HOWE C., ROSE H. M. Structure and development of viruses as observed in the electron microscope. V. Western equine encephalomyelitis virus. J Exp Med. 1961 Jan 1;113:219–234. doi: 10.1084/jem.113.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. MUSSGAY M. STUDIES ON THE STRUCTURE OF A HEMAGGLUTINATING COMPONENT OF A GROUP A ARBO VIRUS (SINDBIS). Virology. 1964 Aug;23:573–581. doi: 10.1016/0042-6822(64)90241-7. [DOI] [PubMed] [Google Scholar]
  23. MUSSGAY M., WEIBEL J. Electron microscopic demonstration of purified Venezuelan equine encephalitis virus. Virology. 1963 Jan;19:109–112. doi: 10.1016/0042-6822(63)90032-1. [DOI] [PubMed] [Google Scholar]
  24. Mussgay M., Bergold G. H., Weiland E., Ueberschär S. Preparation and evaluation of inactivated Venezuelan equine encephalitis vaccines. Zentralbl Veterinarmed B. 1972 Jun;19(6):511–517. doi: 10.1111/j.1439-0450.1972.tb00430.x. [DOI] [PubMed] [Google Scholar]
  25. Osterrieth P. M., Calberg-Bacq C. M. Changes in morphology, infectivity and haemagglutinating activity of Semliki Forest virus produced by the treatment with caseinase C from Streptomyces albus G. J Gen Microbiol. 1966 Apr;43(1):19–30. doi: 10.1099/00221287-43-1-19. [DOI] [PubMed] [Google Scholar]
  26. PFEFFERKORN E. R., HUNTER H. S. PURIFICATION AND PARTIAL CHEMICAL ANALYSIS OF SINDBIS VIRUS. Virology. 1963 Jul;20:433–445. doi: 10.1016/0042-6822(63)90092-8. [DOI] [PubMed] [Google Scholar]
  27. Pedersen C. E., Jr, Slocum D. R., Levitt N. H. Chromatography of Venezuelan equine encephalomyelitis virus strains on calcium phosphate. Appl Microbiol. 1972 Jul;24(1):91–95. doi: 10.1128/am.24.1.91-95.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Pedersen C. E., Jr, Slocum D. R., Robinson D. M. Comparative studies of plaque variantes derived from a Florida strain of Venezuelan equine encephlomyelitis virus. Infect Immun. 1972 Nov;6(5):779–784. doi: 10.1128/iai.6.5.779-784.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  30. Shapiro D., Brandt W. E., Cardiff R. D., Russell P. K. The proteins of Japanese encephalitis virus. Virology. 1971 Apr;44(1):108–124. doi: 10.1016/0042-6822(71)90158-9. [DOI] [PubMed] [Google Scholar]
  31. Simpson R. W., Hauser R. E. Basic structure of group A arbovirus strains Middelburg, Sindbis, and Semliki Forest examined by negative staining. Virology. 1968 Feb;34(2):358–361. doi: 10.1016/0042-6822(68)90248-1. [DOI] [PubMed] [Google Scholar]
  32. Sreevalsan T., Allen P. T. Replication of Western equine encephalomyelitis virus. II. Cytoplasmic structure involved in the synthesis and development of the virions. J Virol. 1968 Oct;2(10):1038–1046. doi: 10.1128/jvi.2.10.1038-1046.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Strauss J. H., Jr, Burge B. W., Darnell J. E. Carbohydrate content of the membrane protein of Sindbis virus. J Mol Biol. 1970 Feb 14;47(3):437–448. doi: 10.1016/0022-2836(70)90313-x. [DOI] [PubMed] [Google Scholar]
  34. Strauss J. H., Jr, Burge B. W., Darnell J. E. Sindbis virus infection of chick and hamster cells: synthesis of virus-specific proteins. Virology. 1969 Mar;37(3):367–376. doi: 10.1016/0042-6822(69)90220-7. [DOI] [PubMed] [Google Scholar]
  35. Strauss J. H., Jr, Burge B. W., Pfefferkorn E. R., Darnell J. E., Jr Identification of the membrane protein and "core" protein of Sindbis virus. Proc Natl Acad Sci U S A. 1968 Feb;59(2):533–537. doi: 10.1073/pnas.59.2.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Stromberg K. Surface-active agents for isolation of the core component of avian myeloblastosis virus. J Virol. 1972 Apr;9(4):684–697. doi: 10.1128/jvi.9.4.684-697.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Yin F. H., Lockart R. Z., Jr Maturation defects in temperature-sensitive mutants of Sindbis virus. J Virol. 1968 Jul;2(7):728–737. doi: 10.1128/jvi.2.7.728-737.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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