Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1974 Sep;10(3):416–421. doi: 10.1128/iai.10.3.416-421.1974

Bactericidal Activity of Blood of Rabbits Vaccinated With Homologous Antigens of Campylobacter fetus (Vibrio fetus) 1

Maryon Border a, L L Myers a, B D Firehammer a
PMCID: PMC422969  PMID: 4609901

Abstract

Rabbits were vaccinated with the following Campylobacter fetus var. venerealis (Vibrio fetus) antigens: whole-cell (WC), autoclaved (A), boiled (B), and purified postgrowth broth (PGB). Bactericidal activity of freshly drawn heparinized blood against the organism was determined after each vaccination. In all cases bactericidal activity of the blood of vaccinated rabbits was higher than for nonvaccinated rabbits. The in vitro bactericidal activity of the blood was determined in two separate experiments. In experiment I the bactericidal activity of the blood of rabbits vaccinated with PGB antigen was the same as that of rabbits vaccinated with WC antigen and higher than that of rabbits vaccinated with A antigen after the third vaccination. In experiment II the bactericidal activity of blood of rabbits vaccinated with PGB antigen was the same as that of those vaccinated with WC antigen after the second and third vaccinations and higher than for rabbits vaccinated with A antigen after the third vaccination. Blood of rabbits vaccinated with A antigen was less bactericidal than blood of rabbits vaccinated with B antigen after the third vaccination, indicating the presence of a surface antigen destroyed by autoclaving but not by boiling. The in vivo and in vitro whole blood bactericidal tests are more sensitive for measuring the response of rabbits vaccinated with WC, B, A, or PGB antigens than is the plate agglutination test.

Full text

PDF
416

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barta O., Barta V., Ingram D. G., Hubbert W. T. Bactericidal activity of bovine fetal serum against smooth and rough strains of Escherichia coli. Am J Vet Res. 1972 Apr;33(4):731–740. [PubMed] [Google Scholar]
  2. Berg R. L., Firehammer B. D., Hawkins W. W. Protective antigens of Vibrio fetus in sheep. Am J Vet Res. 1969 Aug;30(8):1289–1292. [PubMed] [Google Scholar]
  3. Berg R. L., Jutila J. W., Firehammer B. D. A revised classification of Vibrio fetus. Am J Vet Res. 1971 Jan;32(1):11–22. [PubMed] [Google Scholar]
  4. Bjornson A. B., Michael J. G. Contribution of humoral and cellular factors to the resistance to experimental infection by Pseudomonas aeruginosa in mice. I. Interaction between immunoglobulins, heat-labile serum factors, and phagocytic cells in the killing of bacteria. Infect Immun. 1971 Oct;4(4):462–467. doi: 10.1128/iai.4.4.462-467.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carrier S. P., Kramer T. T. Rate of Clearance of Vibrio fetus var. venerealis in the Rabbit. Infect Immun. 1971 Jun;3(6):815–818. doi: 10.1128/iai.3.6.815-818.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Firehammer B. D., Border M. Isolation of temperate bacteriophages from Vibrio fetus. Am J Vet Res. 1968 Nov;29(11):2229–2235. [PubMed] [Google Scholar]
  7. Ghadirian E., Hoghooghi N. The presence of snails of veterinary importance in Isfahan, Iran. Br Vet J. 1973 Mar-Apr;129(2):1–3. doi: 10.1016/s0007-1935(17)36560-0. [DOI] [PubMed] [Google Scholar]
  8. Glynn A. A., Ward M. E. Nature and Heterogeneity of the Antigens of Neisseria gonorrhoeae Involved in the Serum Bactericidal Reaction. Infect Immun. 1970 Aug;2(2):162–168. doi: 10.1128/iai.2.2.162-168.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnston R. B., Jr, Klemperer M. R., Alper C. A., Rosen F. S. The enhancement of bacterial phagocytosis by serum. The role of complement components and two cofactors. J Exp Med. 1969 Jun 1;129(6):1275–1290. doi: 10.1084/jem.129.6.1275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Joos R. W., Hall W. H. Bactericidal action of fresh rabbit blood against Brucella abortus. J Bacteriol. 1968 Oct;96(4):881–885. doi: 10.1128/jb.96.4.881-885.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Myers L. L., Berg R. L., Firehammer B. D. Immunoserologic properties of antigens from Vibrio fetus of ovine origin. Am J Vet Res. 1970 Oct;31(10):1773–1777. [PubMed] [Google Scholar]
  12. Myers L. L. Purification and Partial Characterization of a Vibrio fetus Immunogen. Infect Immun. 1971 Apr;3(4):562–566. doi: 10.1128/iai.3.4.562-566.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Newman S. L., Waldo B., Johnston R. B., Jr Separation of serum bactericidal and opsonizing activities for Haemophilus influenzae type b. Infect Immun. 1973 Sep;8(3):488–490. doi: 10.1128/iai.8.3.488-490.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pike R. M. Antibody heterogeneity and serological reactions. Bacteriol Rev. 1967 Jun;31(2):157–174. doi: 10.1128/br.31.2.157-174.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reynolds B. L., Pruul H. Protective role of smooth lipopolysaccharide in the serum bactericidal reaction. Infect Immun. 1971 Dec;4(6):764–771. doi: 10.1128/iai.4.6.764-771.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reynolds B. L., Pruul H. Sensitization of complement-resistant smooth gram-negative bacterial strains. Infect Immun. 1971 Mar;3(3):365–372. doi: 10.1128/iai.3.3.365-372.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stalheim O. H. Virulent and avirulent Leptospires: biochemical activities and survival in blood. Am J Vet Res. 1971 Jun;32(6):843–849. [PubMed] [Google Scholar]
  18. Taylor P. W., Roberts A. P., Gower P. E. Evaluation of a technique for the estimation of serum bactericidal activity against Gram-negative organisms. Med Lab Technol. 1972 Jul;29(3):272–279. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES