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. 1974 Sep;10(3):430–436. doi: 10.1128/iai.10.3.430-436.1974

Stimulation of Peripheral Blood Lymphocytes by Herpes Simplex Virus in Vitro

M Scriba 1
PMCID: PMC422971  PMID: 4372170

Abstract

A method for stimulating sensitized lymphocytes by inactivated herpes simplex virus was established by using cultures of washed whole blood cells. The development of the immune response of herpes simplex virus-infected guinea pigs was examined at different times in the 4-month period after infection. Humoral and cellular immune responses were compared in individual animals by measuring lymphocyte stimulation ratios and neutralizing serum antibodies.

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Selected References

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  1. ANDERSON W. A., KILBOURNE E. D. A herpes simplex skin test diagnostic antigen of low protein content from cell culture fluid. J Invest Dermatol. 1961 Jul;37:25–28. [PubMed] [Google Scholar]
  2. Adler W. H., Rabinowitz S. G. Host defenses during primary Venezuelan equine encephalomyelitis virus infection in mice. II. In vitro methods for the measurement and qualitation of the immune response. J Immunol. 1973 May;110(5):1354–1362. [PubMed] [Google Scholar]
  3. Banks K. L. The effect of antibody on antigen-induced lymphocyte transformation. J Immunol. 1973 Mar;110(3):709–716. [PubMed] [Google Scholar]
  4. Bryan J. H., Hybertson R. L. The in vitro stimulation of lymphocytes from peripheral blood and lymph nodes of the laboratory mouse. Cytogenetics. 1972;11(1):25–34. doi: 10.1159/000130173. [DOI] [PubMed] [Google Scholar]
  5. Davies D. H., Carmichael L. E. Role of cell-mediated immunity in the recovery of cattle from primary and recurrent infections with infectious bovine rhinotracheitis virus. Infect Immun. 1973 Oct;8(4):510–518. doi: 10.1128/iai.8.4.510-518.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elfenbein G. J., Rosenberg G. L. In vitro proliferation of rabbit bone marrow-derived and thymus-derived lymphocytes in response to vaccinia virus. Cell Immunol. 1973 Jun;7(3):516–521. doi: 10.1016/0008-8749(73)90216-5. [DOI] [PubMed] [Google Scholar]
  7. Elfenbein G. J., Shevach E. M., Green I. Proliferation by bone marrow-derived lymphocytes in response to antigenic stimulation in vitro. J Immunol. 1972 Oct;109(4):870–874. [PubMed] [Google Scholar]
  8. Gerber P., Lucas S. J. In vitro stimulation of human lymphocytes by Epstein-Barr virus. Cell Immunol. 1972 Oct;5(2):318–324. doi: 10.1016/0008-8749(72)90057-3. [DOI] [PubMed] [Google Scholar]
  9. Griffin D. E., Johnson R. T. Cellular immune response to viral infection: in vitro studies of lymphocytes from mice infected with Sindbis virus. Cell Immunol. 1973 Dec;9(3):426–434. doi: 10.1016/0008-8749(73)90057-9. [DOI] [PubMed] [Google Scholar]
  10. Han T., Pauly J. Simplified whole blood method for evaluating in vitro lymphocyte reactivity of laboratory animals. Clin Exp Immunol. 1972 May;11(1):137–142. [PMC free article] [PubMed] [Google Scholar]
  11. Harris G. Further studies of antigen stimulation of deoxyribonucleic acid synthesis in rabbit spleen cell cultures. II. The effects of specific antibody. Immunology. 1968 Mar;14(3):415–423. [PMC free article] [PubMed] [Google Scholar]
  12. Heiniger H. J., Wolf J. M., Chen H. W., Meier H. A micromethod for lymphoblastic transformation of mouse lymphocytes from peripheral blood. Proc Soc Exp Biol Med. 1973 May;143(1):6–11. doi: 10.3181/00379727-143-37242. [DOI] [PubMed] [Google Scholar]
  13. Hinz C. F., Jr, Daniel T. M., Baum G. L. Quantitative aspects of the stimulation of lymphocytes by tuberculin purified protein derivative. Int Arch Allergy Appl Immunol. 1970;38(2):119–129. doi: 10.1159/000230265. [DOI] [PubMed] [Google Scholar]
  14. Hirsch M. S., Murphy F. A. Effects of anti-lymphoid sera on viral infections. Lancet. 1968 Jul 6;2(7558):37–40. doi: 10.1016/s0140-6736(68)92904-8. [DOI] [PubMed] [Google Scholar]
  15. JAWETZ E., COLEMAN V., ALLENDE M. F. Studies on herpes simplex virus. II. A soluble antigen of herpes virus possessing skin-reactive properties. J Immunol. 1951 Sep;67(3):197–205. [PubMed] [Google Scholar]
  16. Jevitz M. A., Ekstedt R. D. Correlation of lymphocyte transformation with the in vivo immune responsiveness of rabbits. J Immunol. 1971 Feb;106(2):494–505. [PubMed] [Google Scholar]
  17. Junge U., Hoekstra J., Wolfe L., Deinhardt F. Microtechnique for quantitative evaluation of in vitro lymphocyte transformation. Clin Exp Immunol. 1970 Sep;7(3):431–437. [PMC free article] [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Nathanson N., Cole G. A. Immunosuppression: a means to assess the role of the immune response in acute virus infections. Fed Proc. 1971 Nov-Dec;30(6):1822–1830. [PubMed] [Google Scholar]
  20. Oppenheim J. J. Modulation of in vitro lymphocyte transformation by antibodies: enhancement by antigen-antibody complexes and inhibition by antibody excess. Cell Immunol. 1972 Mar;3(3):341–360. doi: 10.1016/0008-8749(72)90243-2. [DOI] [PubMed] [Google Scholar]
  21. Oppenheim J. J. Relationship of in vitro lymphocyte transformation to delayed hypersensitivity in guinea pigs and man. Fed Proc. 1968 Jan-Feb;27(1):21–28. [PubMed] [Google Scholar]
  22. Park B. H., Good R. A. A new micromethod for evaluating lymphocyte responses to phytohemagglutinin: quantitative analysis of the function of thymus-dependent cells. Proc Natl Acad Sci U S A. 1972 Feb;69(2):371–373. doi: 10.1073/pnas.69.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Paty D. W., Hughes D. Lymphocyte transformation using whole blood cultures: an analysis of responses. J Immunol Methods. 1972 Nov;2(1):99–114. doi: 10.1016/0022-1759(72)90022-1. [DOI] [PubMed] [Google Scholar]
  24. Pauly J. L., Sokal J. E. A simplified technique for in vitro studies of lymphocyte reactivity. Proc Soc Exp Biol Med. 1972 May;140(1):40–44. doi: 10.3181/00379727-140-36391. [DOI] [PubMed] [Google Scholar]
  25. Phillips S. M., Zweiman B. Characteristics of the in vitro response of guinea pig blood lymphocytes to PHA and antigen. J Immunol. 1970 Jul;105(1):204–214. [PubMed] [Google Scholar]
  26. Plummer G. Isolation of herpesviruses from trigeminal ganglia of man, monkeys, and cats. J Infect Dis. 1973 Sep;128(3):345–347. doi: 10.1093/infdis/128.3.345. [DOI] [PubMed] [Google Scholar]
  27. Ricci M., Romagnani S., Passaleva A., Biliotti G. Lymphocyte transformation and macrophage migration in guinea-pigs immunized with Freund's complete adjuvant. Clin Exp Immunol. 1969 Dec;5(6):659–667. [PMC free article] [PubMed] [Google Scholar]
  28. Rosenberg G. L., Farber P. A., Notkins A. L. In vitro stimulation of sensitized lymphocytes by herpes simplex virus and vaccinia virus. Proc Natl Acad Sci U S A. 1972 Mar;69(3):756–760. doi: 10.1073/pnas.69.3.756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Ruben F. L., Jackson G. G., Gotoff S. P. Humoral and cellular response in humans after immunization with influenza vaccine. Infect Immun. 1973 Apr;7(4):594–596. doi: 10.1128/iai.7.4.594-596.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Russell A. S., Maini R. A., Bailey M., Dumonde D. C. Cell-mediated immunity to Varicella-Zoster antigen in acute Herpes zoster (shingles). Clin Exp Immunol. 1973 Jun;14(2):181–185. [PMC free article] [PubMed] [Google Scholar]
  31. Simons M. J., Fitzgerald M. G. Rubella virus and human lymphocytes in culture. Lancet. 1968 Nov 2;2(7575):937–940. doi: 10.1016/s0140-6736(68)91167-7. [DOI] [PubMed] [Google Scholar]
  32. Smith K. A., Chess L., Mardiney M. R., Jr The characteristics of lymphocyte tritiated thymidine incorporation in response to mumps virus. Cell Immunol. 1972 Dec;5(4):597–603. doi: 10.1016/0008-8749(72)90111-6. [DOI] [PubMed] [Google Scholar]
  33. Smith K. A., Chess L., Mardiney M. R., Jr The relationship between rubella hemagglutination inhibition antibody (HIA) and rubella induced in vitro lymphocyte tritiated thymidine incorporation. Cell Immunol. 1973 Aug;8(2):321–327. doi: 10.1016/0008-8749(73)90121-4. [DOI] [PubMed] [Google Scholar]
  34. Steele R. W., Hensen S. A., Vincent M. M., Fuccillo D. A., Bellanti J. A. A 51 Cr microassay technique for cell-mediated immunity to viruses. J Immunol. 1973 Jun;110(6):1502–1510. [PubMed] [Google Scholar]
  35. Thorbecke G. J., Siskind G. W. Effect of specific antibody on the antigen-induced proliferative response of rabbit lymph node cells. J Immunol. 1973 Mar;110(3):648–651. [PubMed] [Google Scholar]
  36. Wallis C., Melnick J. L. Herpesvirus neutralization: induction of the persistent fraction by insufficient antibody. Virology. 1970 Sep;42(1):128–137. doi: 10.1016/0042-6822(70)90245-x. [DOI] [PubMed] [Google Scholar]
  37. Wilton J. M., Ivanyi L., Lehner T. Cell-mediated immunity in Herpesvirus hominis infections. Br Med J. 1972 Mar 18;1(5802):723–726. doi: 10.1136/bmj.1.5802.723. [DOI] [PMC free article] [PubMed] [Google Scholar]

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