Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1974 Sep;10(3):458–463. doi: 10.1128/iai.10.3.458-463.1974

Local Antibody Production Against the Murine Toxin of Yersinia pestis in a Golf Ball-Induced Granuloma

Robyn P Hillam 1,2, Robert P Tengerdy 1,2, George L Brown 1,2
PMCID: PMC422975  PMID: 4214770

Abstract

The method of Garra and Baygorria, for localized antibody production, has been adapted for obtaining high-titered and monospecific antibodies against the murine toxin of Yersinia pestis. A hollow, perforated plastic golf ball surgically implanted under the skin of rabbits induced the formation of a granuloma. When the murine toxin of Y. pestis was injected directly into the granulomatous cavity, an increased amount of antibody was found in the granuloma fluid as compared with serum or with serum antibody obtained by conventional immunization. The granuloma antibody consisted mainly of immunoglobulin G, probably produced locally by lymphoid cells of the granuloma. The immune granuloma fluid and the granuloma tissue were rich in plasma cells and lymphocytes. The chemical composition of the granuloma fluid indicated that it was a transudate.

Full text

PDF
458

Images in this article

459Image
on p.459
461Image
on p.461
462Image
on p.462

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AJL S. J., REEDAL J. S., DURRUM E. L., WARREN J. Studies on plague. I. Purification and properties of the toxin of Pasteurella pestis. J Bacteriol. 1955 Aug;70(2):158–169. doi: 10.1128/jb.70.2.158-169.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BAKER E. E., SOMMER H., FOSTER L. E., MEYER E., MEYER K. F. Studies on immunization against plague. I. The isolation and characterization of the soluble antigen of Pasteurella pestis. J Immunol. 1952 Feb;68(2):131–145. [PubMed] [Google Scholar]
  3. GARRA A., CENDON DE BAYGORRIA G., MORATO MANARO J., PASEYRO P. [The immunity granuloma as a method for the production of human growth hormone antibodies]. An Fac Med Univ Repub Montev Urug. 1960;45:339–341. [PubMed] [Google Scholar]
  4. GARRA A., CENDON DE BAYGORRIA G. [Local production of antibodies]. An Fac Med Univ Repub Montev Urug. 1959;44:544–547. [PubMed] [Google Scholar]
  5. Merrill D., Hartley T. F., Claman H. N. Electroimmunodiffusion (EID): a simple, rapid method for quantitation of immunoglobulins in dilute biological fluids. J Lab Clin Med. 1967 Jan;69(1):151–159. [PubMed] [Google Scholar]
  6. PASEYRO P., NEGRIN E., MORATO MANARO J., GROSSO O. F. [The immunological granuloma of Garra and Baygorria as a method of obtaining anti-thyroid antibodies in the rabbit]. An Fac Med Univ Repub Montev Urug. 1961;46:297–303. [PubMed] [Google Scholar]
  7. SOMMA MOREIRA R. E., TOSI H. C. [The Garra-Baygorria immune granuloma and its use in virology]. An Fac Med Univ Repub Montev Urug. 1962;47:152–156. [PubMed] [Google Scholar]
  8. SPEIRS R. S., SPEIRS E. E. CELLULAR LOCALIZATION OF RADIOACTIVE ANTIGEN IN IMMUNIZED AND NONIMMUNIZED MICE. J Immunol. 1963 Apr;90:561–575. [PubMed] [Google Scholar]
  9. Tengerdy R. P., Hillam R. P. Quantitative differentiation of Yersinia pestis strains by their murine toxin and fraction I contents. Bull World Health Organ. 1973;48(3):279–287. [PMC free article] [PubMed] [Google Scholar]
  10. WARREN J., WALZ U., REEDAL J. S., AJL S. J. Studies on plague. II. Immunological properties of purified Pasteurella pestis toxin. J Bacteriol. 1955 Aug;70(2):170–176. doi: 10.1128/jb.70.2.170-176.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES