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. 1974 Sep;10(3):475–480. doi: 10.1128/iai.10.3.475-480.1974

Differentiation of Four Adenovirus Types by Macrophage Migration Inhibition Tests

James F Novotny a,1, Frank M Hetrick a, David Via a
PMCID: PMC422978  PMID: 4426697

Abstract

The macrophage migration inhibition (MMI) test was found to be a satisfactory procedure for distinguishing between adenovirus types 1, 4, 5, and 7. Highly purified virus preparations were used for the sensitization of Hartley strain guinea pigs, whereas the MMI test antigen consisted of crude virus preparations grown in KB cells. With all four virus types, a significantly greater MMI response was noted when peritoneal exudate cells were exposed to the homologous sensitizing antigen as compared to that obtained with the three heterologous antigens. Studies with adenovirus type 1 indicate that sensitizing doses between 70 and 150 μg of viral protein per guinea pig gave the optimal MMI response. Doses below 70 μg did not stimulate the delayed response, whereas doses above 120 μg produced MMI reactions which were nonspecific, as differences between homologous and heterologous antigens were not demonstrable.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benton C. V., Reichelderfer C. F., Hetrick F. M. Differentiation of Trichoplusia ni MEV and Autographa californica MEV by macrophage migration inhibition tests. J Invertebr Pathol. 1973 Jul;22(1):42–49. doi: 10.1016/0022-2011(73)90008-6. [DOI] [PubMed] [Google Scholar]
  2. DAVID J. R., AL-ASKARI S., LAWRENCE H. S., THOMAS L. DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS. J Immunol. 1964 Aug;93:264–273. [PubMed] [Google Scholar]
  3. GEORGE M., VAUGHAN J. H. In vitro cell migration as a model for delayed hypersensitivity. Proc Soc Exp Biol Med. 1962 Nov;111:514–521. doi: 10.3181/00379727-111-27841. [DOI] [PubMed] [Google Scholar]
  4. Hughes D. Macrophage migration inhibition test: a critical examination of the technique using a polythene capillary tubing micromethod. J Immunol Methods. 1972 Aug;1(4):403–424. doi: 10.1016/0022-1759(72)90034-8. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. PEREIRA H. G. Typing of adenoidal-pharyngeal-conjunctival (APC) viruses by complement-fixation. J Pathol Bacteriol. 1956 Jul;72(1):105–109. [PubMed] [Google Scholar]
  7. ROSEN L. A hemagglutination-inhibition technique for typing adenoviruses. Am J Hyg. 1960 Jan;71:120–128. doi: 10.1093/oxfordjournals.aje.a120085. [DOI] [PubMed] [Google Scholar]
  8. Schlossman S. F., Ben-Efraim S., Yaron A., Sober H. A. Immunochemical studies on the antigenic determinants required to elicit delayed and immediate hypersensitivity reactions. J Exp Med. 1966 Jun 1;123(6):1083–1095. doi: 10.1084/jem.123.6.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Stevens D. A., Schaeffer M., Fox J. P., Brandt C. D., Romano M. Standardization and certification of reference antigens and antisera for 30 human adenovirus serotypes. Am J Epidemiol. 1967 Nov;86(3):617–633. doi: 10.1093/oxfordjournals.aje.a120771. [DOI] [PubMed] [Google Scholar]
  10. Thor D. E., Dray S. A correlate of human delayed hypersensitivity: specific inhibition of capillary tube migration of sensitized human lymph node cells by tuberculin and histoplasmin. J Immunol. 1968 Jul;101(1):51–61. [PubMed] [Google Scholar]
  11. Tompkins W. A., Adams C., Rawls W. E. An in vitro measure of cellular immunity to fibroma virus. J Immunol. 1970 Feb;104(2):502–510. [PubMed] [Google Scholar]

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