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. 1974 Sep;10(3):528–534. doi: 10.1128/iai.10.3.528-534.1974

Reduced Nicotinamide Adenine Dinucleotide and Reduced Nicotinamide Adenine Dinucleotide Phosphate Diaphorase Activity in Human Polymorphonuclear Leukocytes

Lawrence R DeChatelet 1,2, Linda C McPhail 1,2, Debra Mullikin 1,2, Charles E McCall 1,2
PMCID: PMC422986  PMID: 4154306

Abstract

Total reduced nicotinamide adenine dinucleotide (NADH) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase activities were examined in human neutrophils. Approximately two-thirds of each enzyme activity was located in the granule fraction with the remainder in the soluble. The activities in a 27,000 × g supernatant from a sonic extract of human polymorphonuclear leukocytes were characterized. Both NADH and NADPH diaphorase were insensitive to cyanide and azide and showed greater activity at acid pH. Km values for nitroblue tetrazolium were not markedly different (33 μM with NADH and 12 μM with NADPH), but there was a 40-fold difference in Km for the reduced pyridine nucleotides (10 μM with NADH and 400 μM for NADPH). Since the intracellular concentration of both nucleotides is estimated to be about 50 μM, it is much more likely, from a kinetic argument, that the respiratory burst of phagocytosis is intiated by the oxidation of NADH rather than of NADPH.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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