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. 1974 Oct;10(4):765–771. doi: 10.1128/iai.10.4.765-771.1974

Antigen-Induced Proliferative Response to Murine Thymus Cells in Vitro

L R Lyle 1, S A Eisen 1, C W Parker 1
PMCID: PMC423020  PMID: 4547743

Abstract

Thymus cells from 5- to 6-week-old normal (unimmunized) BALB/c mice showed an increased incorporation of [3H]thymidine in the presence of 2,4-dinitrophenyl-bovine serum albumin, fluorescein-bovine serum albumin, and bovine serum albumin (BSA) in tissue culture. The concentrations of antigen (BSA and haptenated proteins) required for stimulation were approximately 25- to 50-fold higher than those of the nonspecific mitogen, concanavalin A. In contrast to the stimulation by concanavalin A, which was maximal at 24 to 72 h, the stimulation by antigen was most marked earlier in the culture period (6 to 24 h). The BSA response was diminished to a statistically significant degree (especially at low BSA concentrations) in thymocytes from animals injected 72 h previously with BSA, indicating that the stimulation is immunologically specific.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATKINS E., ALLISON F., Jr, SMITH M. R., WOOD W. B., Jr Studies on the antipyretic action of cortisone in pyrogen-induced fever. J Exp Med. 1955 Apr 1;101(4):353–366. doi: 10.1084/jem.101.4.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ATKINS E., WOOD W. B., Jr Studies on the pathogenesis of fever. II. Identification of an endogenous pyrogen in the blood stream following the injection of typhoid vaccine. J Exp Med. 1955 Nov 1;102(5):499–516. doi: 10.1084/jem.102.5.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adler W. H., Takiguchi T., Marsh B., Smith R. T. Cellular recognition by mouse lymphocytes in vitro. I. Definition of a new technique and results of stimulation by phytohemagglutinin and specific antigens. J Exp Med. 1970 Jun 1;131(6):1049–1078. doi: 10.1084/jem.131.6.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Andersson J., Sjöberg O., Möller G. Reversibility of high dose unresponsiveness to concanavalin A in thymus lymphocytes. Immunology. 1972 Oct;23(4):637–646. [PMC free article] [PubMed] [Google Scholar]
  5. Chiller J. M., Habicht G. S., Weigle W. O. Cellular sites of immunologic unresponsiveness. Proc Natl Acad Sci U S A. 1970 Mar;65(3):551–556. doi: 10.1073/pnas.65.3.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cooper J. F., Levin J., Wagner H. N., Jr Quantitative comparison of in vitro and in vivo methods for the detection of endotoxin. J Lab Clin Med. 1971 Jul;78(1):138–148. [PubMed] [Google Scholar]
  7. Davies A. J., Carter R. L., Leuchars E., Wallis V., Dietrich F. M. The morphology of immune reactions in normal, thymectomized and reconstituted mice. 3. Response to bacterial antigens: salmonellar flagellar antigen and pneumococcal plysaccharide. Immunology. 1970 Dec;19(6):945–957. [PMC free article] [PubMed] [Google Scholar]
  8. Doria G., Martinozzi M., Agarossi G., Di Pietro S. In vitro primary immune response resulting from the interaction between bone marrow-derived and thymus cells. Experientia. 1970 Apr 15;26(4):410–411. doi: 10.1007/BF01896919. [DOI] [PubMed] [Google Scholar]
  9. Gershon R. K., Maurer P. H., Merryman C. F. A cellular basis for genetically controlled immunologic unresponsiveness in mice: tolerance induction in T-cells. Proc Natl Acad Sci U S A. 1973 Jan;70(1):250–254. doi: 10.1073/pnas.70.1.250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Howe M. L., Manziello B. Ontogenesis of the in vitro response of murine lymphoid cells to cellular antigens and phytomitogens. J Immunol. 1972 Sep;109(3):534–539. [PubMed] [Google Scholar]
  11. Katz D. H., Benacerraf B. The regulatory influence of activated T cells on B cell responses to antigen. Adv Immunol. 1972;15:1–94. doi: 10.1016/s0065-2776(08)60683-5. [DOI] [PubMed] [Google Scholar]
  12. Krüger J., Gershon R. K. DNA synthetic response of thymocytes to a variety of antigens. J Immunol. 1972 Mar;108(3):581–585. [PubMed] [Google Scholar]
  13. Lawrence D. A., Spiegelberg H. L., Weigle W. O. 2,4-Dinitrophenyl receptors on mouse thymus and spleen cells. J Exp Med. 1973 Feb 1;137(2):470–482. doi: 10.1084/jem.137.2.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lyle L. R., Eisen S. A., Parker C. W. Mixed leukocyte culture reactions in mouse thymus cells. Immunol Commun. 1973;2(3):255–263. doi: 10.3109/08820137309022797. [DOI] [PubMed] [Google Scholar]
  15. MacManus J. P., Whitfield J. F. Mediation of the mitogenic action of growth hormone by adenosine 3'5'-monophosphate (cyclic AMP). Proc Soc Exp Biol Med. 1969 Nov;132(2):409–412. doi: 10.3181/00379727-132-34226. [DOI] [PubMed] [Google Scholar]
  16. McNeill T. A. Antigenic stimulation of bone marrow colony forming cells. II. Properties of a serum factor responsible for antigenic enhancement of colonies. Immunology. 1970 Jan;18(1):49–59. [PMC free article] [PubMed] [Google Scholar]
  17. Mond J. J., Thorbecke G. J. Proliferative response of normal rabbit and mouse bone marrow cells in vitro. Cell Immunol. 1972 Nov;5(3):480–486. doi: 10.1016/0008-8749(72)90074-3. [DOI] [PubMed] [Google Scholar]
  18. Singhal S. K., Richter M. Cells involved in the immune response. IV. The response of normal and immune rabbit bone marrow and lymphoid tissue lymphocytes to antigens in vitro. J Exp Med. 1968 Nov 1;128(5):1099–1128. doi: 10.1084/jem.128.5.1099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stobo J. D., Rosenthal A. S., Paul W. E. Functional heterogeneity of murine lymphoid cells. I. Responsiveness to and surface binding of concanavalin A and phytohemagglutinin. J Immunol. 1972 Jan;108(1):1–17. [PubMed] [Google Scholar]
  20. Takiguchi T., Adler W. H., Smith R. T. Cellular recognition in vitro by mouse lymphocytes. Effects of neonatal thymectomy and thymus graft restoration on alloantigen and PHA stimulation of whole and gradient-separated subpopulations of spleen cells. J Exp Med. 1971 Jan 1;133(1):63–80. doi: 10.1084/jem.133.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]

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