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. 1974 Nov;10(5):991–995. doi: 10.1128/iai.10.5.991-995.1974

Production of Hemolysin and Bacteriolysin in a Synthetic Medium by Streptococcus faecalis var. zymogenes1

Benjamin Appelbaum a, Leonard N Zimmerman a
PMCID: PMC423050  PMID: 16558115

Abstract

A synthetic medium has been developed in which Streptococcus faecalis var. zymogenes X-14 elaborates a lysin. The medium consists of 18 amino acids, lactose, six vitamins, adenine, guanine, uracil, and six salts. Glucose, and K2HPO4 in excess of 0.5% (wt/vol), were inhibitory to lysin production; increasing concentrations of l-arginine-hydrochloride gave increasing yields of lytic activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLEIWEIS A. S., ZIMMERMAN L. N. PROPERTIES OF PROTEINASE FROM STREPTOCOCCUS FAECALIS VAR. LIQUEFACIENS. J Bacteriol. 1964 Sep;88:653–659. doi: 10.1128/jb.88.3.653-659.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BROCK T. D., DAVIE J. M. PROBABLE IDENTITY OF A GROUP D HEMOLYSIN WITH A BACTERIOCINE. J Bacteriol. 1963 Oct;86:708–712. doi: 10.1128/jb.86.4.708-712.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BROCK T. D., PEACHER B., PIERSON D. SURVEY OF THE BACTERIOCINES OF ENTEROCOCCI. J Bacteriol. 1963 Oct;86:702–707. doi: 10.1128/jb.86.4.702-707.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Basinger S. F., Jackson R. W. Bacteriocin (hemolysin) of Streptococcus zymogenes. J Bacteriol. 1968 Dec;96(6):1895–1902. doi: 10.1128/jb.96.6.1895-1902.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Casas I. A., Zimmerman L. N. Dependence of protease secretion by Streptococcus faecalis var. liquefaciens on arginine and its possible relation to site of synthesis. J Bacteriol. 1969 Jan;97(1):307–312. doi: 10.1128/jb.97.1.307-312.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DEIBEL R. H. THE GROUP D STREPTOCOCCI. Bacteriol Rev. 1964 Sep;28:330–366. doi: 10.1128/br.28.3.330-366.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davie J. M., Brock T. D. Action of streptolysin S, the group D hemolysin, and phospholipase C on whole cells and spheroplasts. J Bacteriol. 1966 Feb;91(2):595–600. doi: 10.1128/jb.91.2.595-600.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Davie J. M., Brock T. D. Effect of teichoic acid on resistance to the membrane-lytic agent of Streptococcus zymogenes. J Bacteriol. 1966 Dec;92(6):1623–1631. doi: 10.1128/jb.92.6.1623-1631.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Duncan J. L., Cho G. J. Production of staphylococcal alpha toxin. II. Glucose repression of toxin formation. Infect Immun. 1972 Nov;6(5):689–694. doi: 10.1128/iai.6.5.689-694.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Granato P. A., Jackson R. W. Bicomponent nature of lysin from Streptococcus zymogenes. J Bacteriol. 1969 Nov;100(2):865–868. doi: 10.1128/jb.100.2.865-868.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Granato P. A., Jackson R. W. Characterization of the A component of Streptococcus zymogenes lysin. J Bacteriol. 1971 Aug;107(2):551–556. doi: 10.1128/jb.107.2.551-556.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Granato P. A., Jackson R. W. Purification and characterization of the L component of Streptococcus zymogenes lysin. J Bacteriol. 1971 Nov;108(2):804–808. doi: 10.1128/jb.108.2.804-808.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HAMMEL J. M., ZIMMERMAN L. N. GROWTH STIMULATION OF STREPTOCOCCUS FAECALIS VAR. LIQUEFACIENS BY CANAVANINE. J Bacteriol. 1963 Sep;86:490–493. doi: 10.1128/jb.86.3.490-493.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HARTMAN R. E., ZIMMERMAN L. N. Effect of the arginine dihydrolase enzyme system on proteinase biosynthesis by Streptococcus faecalis var. liquefaciens. J Bacteriol. 1960 Dec;80:753–761. doi: 10.1128/jb.80.6.753-761.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HARTMAN R. E., ZIMMERMAN L. N., RABIN R. Proteinase biosynthesis by Streptococcus liquefaciens. II. Purine, pyrimidine, and vitamin requirements. Can J Microbiol. 1957 Jun;3(4):553–558. doi: 10.1139/m57-060. [DOI] [PubMed] [Google Scholar]
  16. Higgins M. L., Shockman G. D. Procaryotic cell division with respect to wall and membranes. CRC Crit Rev Microbiol. 1971 May;1(1):29–72. doi: 10.3109/10408417109104477. [DOI] [PubMed] [Google Scholar]
  17. IRWIN J., SEELEY H. W., Jr Titration and partial characterization of a soluble hemolysin of a group D streptococcus. J Bacteriol. 1958 Jul;76(1):29–35. doi: 10.1128/jb.76.1.29-35.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shockman G. D., Thompson J. S., Conover M. J. The autolytic enzyme system of Streptococcus faecalis. II. Partial characterization of the autolysin and its substrate. Biochemistry. 1967 Apr;6(4):1054–1065. doi: 10.1021/bi00856a014. [DOI] [PubMed] [Google Scholar]
  19. Werth J. M., Jackson R. W. Effects of various drugs on hemolytic activity of Streptococcus zymogenes. J Bacteriol. 1971 Nov;108(2):844–848. doi: 10.1128/jb.108.2.844-848.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Woolley D. W., Hutchings B. L. Synthetic Media for Culture of Certain Hemolytic Streptococci. J Bacteriol. 1940 Mar;39(3):287–296. doi: 10.1128/jb.39.3.287-296.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]

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