Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1974 Nov;10(5):1051–1056. doi: 10.1128/iai.10.5.1051-1056.1974

Immunogenicity of Whole Cell and Outer Envelope Leptospiral Vaccines in Hamsters

Russell F Bey 1, Nancy E Auran 1, Russell C Johnson 1
PMCID: PMC423060  PMID: 16558088

Abstract

The immunogenicity of leptospiral whole cell (WC) and outer envelope (OE) vaccines from virulent and avirulent strains was compared in hamsters. The 50% protective dose against death (PD50D) and kidney infection (PD50K) was evaluated for serotypes icterohaemorrhagiae, canicola, pomona, and grippotyphosa. The OE and WC of virulent strains possessed greater immunogenicity than the OE and WC of avirulent strains. More vaccine (<0.1 to 11.0 μg [dry weight] per hamster) was required for the PD50K than for the PD50D (<0.1 to 1.7 μg [dry weight] per hamster). The OE from virulent strains of these four serotypes plus hardjo were combined to form a pentavalent OE vaccine which proved to be satisfactory in immunogenic potency. Duration of immunity was not evaluated.

Full text

PDF
1051

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. L., Johnson R. C. Electron microscopy of immune disruption of leptospires: action of complement and lysozyme. J Bacteriol. 1968 Jun;95(6):2293–2309. doi: 10.1128/jb.95.6.2293-2309.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Auran N. E., Johnson R. C., Ritzi D. M. Isolation of the outer sheath of Leptospira and its immunogenic properties in hamsters. Infect Immun. 1972 Jun;5(6):968–975. doi: 10.1128/iai.5.6.968-975.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Babudieri B., Castelli M., Pisoni F. Comparative tests with formolized and irradiated vaccines against leptospirosis. Bull World Health Organ. 1973 May;48(5):587–590. [PMC free article] [PubMed] [Google Scholar]
  4. Feigin R. D., Lobes L. A., Jr, Anderson D., Pickering L. Human leptospirosis from immunized dogs. Ann Intern Med. 1973 Dec;79(6):777–785. doi: 10.7326/0003-4819-79-6-777. [DOI] [PubMed] [Google Scholar]
  5. Glosser J. W., Johnson R. C., Sulzer C. R., Auran N. E. Immunogenic properties of a leptospiral outer envelope bacterin in hamsters and foxes. Am J Vet Res. 1974 May;35(5):681–684. [PubMed] [Google Scholar]
  6. Johnson R. C., Harris V. G. Differentiation of pathogenic and saprophytic letospires. I. Growth at low temperatures. J Bacteriol. 1967 Jul;94(1):27–31. doi: 10.1128/jb.94.1.27-31.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johnson R. C., Walby J., Henry R. A., Auran N. E. Cultivation of parasitic leptospires: effect of pyruvate. Appl Microbiol. 1973 Jul;26(1):118–119. doi: 10.1128/am.26.1.118-119.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES