Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1974 Dec;10(6):1207–1212. doi: 10.1128/iai.10.6.1207-1212.1974

Investigation of Secretory Immunoglobulins in Saliva from Germfree Mice

J A Molinari 1, J L Ebersole 1, D Platt 1
PMCID: PMC423088  PMID: 4215756

Abstract

We investigated the effect of oral immunization on the serum and salivary immunoglobulin levels in axenic and conventional NIH mice. Specific antibacterial and antifungal antibodies were measured by passive hemagglutination, and differences in the immunoglobulin classes were measured by radial immunodiffusion. After an oral immunization regimen with either intact Escherichia coli or Candida albicans, a significant increase in specific antibody above the base-line reciprocal titer of 32 was observed in conventional mouse saliva. Saliva from axenic mice immunized with the formalized microorganisms yielded reciprocal antibody titers of 256, as compared with an absence of specific antibodies in control animals. Assay of the sera of both groups of immunized mice revealed no increase in antibody levels specific for E. coli lipopolysaccharide or Candidin.

Full text

PDF
1207

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BELLANTI J. A., ARTENSTEIN M. S., BUESCHER E. L. CHARACTERIZATION OF VIRUS NEUTRALIZING ANTIBODIES IN HUMAN SERUM AND NASAL SECRETIONS. J Immunol. 1965 Mar;94:344–351. [PubMed] [Google Scholar]
  2. Benveniste J., Lespinats G., Salomon J. Serum and secretory IgA in axenic and holoxenic mice. J Immunol. 1971 Dec;107(6):1656–1662. [PubMed] [Google Scholar]
  3. Berger R., Ainbender E., Hodes H. L., Zepp H. D., Hevizy M. M. Demonstration of IgA polioantibody in saliva, duodenal fluid and urine. Nature. 1967 Apr 22;214(5086):420–422. doi: 10.1038/214420a0. [DOI] [PubMed] [Google Scholar]
  4. Eddie D. S., Schulkind M. L., Robbins J. B. The isolation and biologic activities of purified secretory IgA and IgG anti-Salmonella typhimurium "O" antibodies from rabbit intestinal fluid and colostrum. J Immunol. 1971 Jan;106(1):181–190. [PubMed] [Google Scholar]
  5. FAHEY J. L., BARTH W. F. THE IMMUNOGLOBULINS OF MICE. 4. SERUM IMMUNOGLOBULIN CHANGES FOLLOWING BIRTH. Proc Soc Exp Biol Med. 1965 Mar;118:596–600. doi: 10.3181/00379727-118-29914. [DOI] [PubMed] [Google Scholar]
  6. Fubara E. S., Freter R. Protection against enteric bacterial infection by secretory IgA antibodies. J Immunol. 1973 Aug;111(2):395–403. [PubMed] [Google Scholar]
  7. Fubara E. S., Freter R. Source and protective function of coproantibodies in intestinal disease. Am J Clin Nutr. 1972 Dec;25(12):1357–1363. doi: 10.1093/ajcn/25.12.1357. [DOI] [PubMed] [Google Scholar]
  8. Gordon H. A., Pesti L. The gnotobiotic animal as a tool in the study of host microbial relationships. Bacteriol Rev. 1971 Dec;35(4):390–429. doi: 10.1128/br.35.4.390-429.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Igel H. J., Bolande R. P. Humoral defense mechanisms in cryptococcosis: substances in normal human serum, saliva, and cerebrospinal fluid affecting the growth of Cryptococcus neoformans. J Infect Dis. 1966 Feb;116(1):75–83. doi: 10.1093/infdis/116.1.75. [DOI] [PubMed] [Google Scholar]
  10. Kono R., Akao A., Sasagawa W., Nomura Y. Studies on the local immunity of intestinal tract of chickens after oral administration of Newcastle disease virus. Prog Immunobiol Stand. 1970;4:330–332. [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Lehner T. Immunofluorescent investigation of Candida albicans antibodies in human saliva. Arch Oral Biol. 1965 Nov-Dec;10(6):975–980. doi: 10.1016/0003-9969(65)90091-9. [DOI] [PubMed] [Google Scholar]
  13. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  14. Miller T. E., Mackaness G. B., Lagrange P. H. Immunopotentiation with BCG. II. Modulation of the response to sheep red blood cells. J Natl Cancer Inst. 1973 Nov;51(5):1669–1676. doi: 10.1093/jnci/51.5.1669. [DOI] [PubMed] [Google Scholar]
  15. Nash D. R., Vaerman J. P., Bazin H., Heremans J. F. Comparative molecular size of mouse IgA in different body fluids. Int Arch Allergy Appl Immunol. 1970;37(2):167–174. doi: 10.1159/000230230. [DOI] [PubMed] [Google Scholar]
  16. Plaut A. G. A review of secretory immune mechanisms. Am J Clin Nutr. 1972 Dec;25(12):1344–1350. doi: 10.1093/ajcn/25.12.1344. [DOI] [PubMed] [Google Scholar]
  17. Reynolds H. Y., Thompson R. E. Pulmonary host defenses. I. Analysis of protein and lipids in bronchial secretions and antibody responses after vaccination with pseudomonas aeruginosa. J Immunol. 1973 Aug;111(2):358–368. [PubMed] [Google Scholar]
  18. Rossen R. D., Wolff S. M., Butler W. T. The antibody response in nasal washings and serum to S. typhosa endotoxin administered intravenously. J Immunol. 1967 Jul;99(1):246–254. [PubMed] [Google Scholar]
  19. Smith C. B., Purcell R. H., Bellanti J. A., Chanock R. M. Protective effect of antibody to parainfluenza type 1 virus. N Engl J Med. 1966 Nov 24;275(21):1145–1152. doi: 10.1056/NEJM196611242752101. [DOI] [PubMed] [Google Scholar]
  20. TOMASI T. B., Jr, TAN E. M., SOLOMON A., PRENDERGAST R. A. CHARACTERISTICS OF AN IMMUNE SYSTEM COMMON TO CERTAIN EXTERNAL SECRETIONS. J Exp Med. 1965 Jan 1;121:101–124. doi: 10.1084/jem.121.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tomasi T. B., Grey H. M. Structure and function of immunoglobulin A. Prog Allergy. 1972;16:81–213. [PubMed] [Google Scholar]
  22. Tomasi T. B., Jr, Bienenstock J. Secretory immunoglobulins. Adv Immunol. 1968;9:1–96. doi: 10.1016/s0065-2776(08)60441-1. [DOI] [PubMed] [Google Scholar]
  23. Waldman R. H., Cruz J. M., Rowe D. S. Intravaginal immunization of humans with Candida albicans. J Immunol. 1972 Oct;109(4):662–664. [PubMed] [Google Scholar]
  24. Waldman R. H., Mann J. J., Kasel J. A. Influenza virus neutralizing antibody in human respiratory secretions. J Immunol. 1968 Jan;100(1):80–85. [PubMed] [Google Scholar]
  25. Waldman R. H., Mann J. J., Small P. A., Jr Immunization against influenza. Prevention of illness in man by aerosolized inactivated vaccine. JAMA. 1969 Jan 20;207(3):520–524. doi: 10.1001/jama.207.3.520. [DOI] [PubMed] [Google Scholar]
  26. Wigley F. M., Fruchtman M. H., Waldman R. H. Aerosol immunization of humans with inactivated parainfluenza type 2 vaccine. N Engl J Med. 1970 Dec 3;283(23):1250–1253. doi: 10.1056/NEJM197012032832303. [DOI] [PubMed] [Google Scholar]
  27. Williams R. C., Gibbons R. J. Inhibition of bacterial adherence by secretory immunoglobulin A: a mechanism of antigen disposal. Science. 1972 Aug 25;177(4050):697–699. doi: 10.1126/science.177.4050.697. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES