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. 1974 Dec;10(6):1302–1306. doi: 10.1128/iai.10.6.1302-1306.1974

Immune Response to Mycobacterium leprae: Plaque-Forming Cells in Mice

R G Navalkar 1,2, P J Patel 1,2, Rekha R Dalvi 1,2, L Levy 1,2
PMCID: PMC423103  PMID: 4611925

Abstract

Intravenous immunization with a cell extract of Mycobacterium leprae produced a primary immune response of considerable magnitude, followed by an equally large response after secondary stimulation, as measured by assay of plaque-forming cells (PFC). Infection with M. leprae or immunization with cell extract by the footpad route produced a lower level of response than that seen in the intravenous group. Identical patterns of response, although not of the same magnitude, were observed after both primary and secondary challenges in the two footpad groups, one infected with viable M. leprae and the other immunized with M. leprae cell extract. The secondary response after a booster dose to all these groups appeared to be an enhanced immunoglobulin M response. Control studies confirmed that the immune response was a direct result of the host-parasite interaction and that the PFC observed resulted from stimulation of antibody-forming cells by antigens of M. leprae. The similarity in time of appearance of peak PFC levels in the two footpad groups may be attributed to the live challenge passing through a latent phase. Alternatively, the challenge is known to contain a large proportion on nonviable cells, and it may also contain soluble M. leprae antigens. Studies of the cross-reactivity of the antigens have extended previous observations on antigens shared between M. leprae and other mycobacterial species. Use of the two antigen-containing fractions of the M. leprae cell extract has suggested that one of the fractions contains some shared antigens, whereas the other has an antigen specific to M. leprae.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dresser D. W., Wortis D. H. Use of an antiglobulin serum to detect cells producing antibody with low haemolytic efficiency. Nature. 1965 Nov 27;208(5013):859–861. doi: 10.1038/208859a0. [DOI] [PubMed] [Google Scholar]
  2. FINKELSTEIN M. S., UHR J. W. SPECIFIC INHIBITION OF ANTIBODY FORMATION BY PASSIVELY ADMINISTERED 19S AND 7S ANTIBODY. Science. 1964 Oct 2;146(3640):67–69. doi: 10.1126/science.146.3640.67. [DOI] [PubMed] [Google Scholar]
  3. Golub E. S., Mishell R. I., Weigle W. O., Dutton R. W. A modification of the hemolytic plaque assay for use with protein antigens. J Immunol. 1968 Jan;100(1):133–137. [PubMed] [Google Scholar]
  4. Johnson H. M., Brenner K., Hall H. E. The use of a wate-soluble carbodiimide as a coupling reagent in the passive hemagglutination test. J Immunol. 1966 Dec;97(6):791–796. [PubMed] [Google Scholar]
  5. Larson C., Baker M. B., Smith D. Plaque-forming cells in the spleens of rabbits immunized with viable BCG bacilli or oil cell wall vaccine from Mycobacterium bovis. Am Rev Respir Dis. 1968 Apr;97(4):715–718. doi: 10.1164/arrd.1968.97.4.715. [DOI] [PubMed] [Google Scholar]
  6. Levy L. Death of Mycobacterium leprae in mice, and the additional effect of dapsone administration. Proc Soc Exp Biol Med. 1970 Dec;135(3):745–749. doi: 10.3181/00379727-135-35134. [DOI] [PubMed] [Google Scholar]
  7. Navalkar R. G. Immunologic analysis of Mycobacterium leprae antigens by means of diffusion-in-gel methods. Int J Lepr Other Mycobact Dis. 1971 Apr-Jun;39(2):105–112. [PubMed] [Google Scholar]
  8. Okuyama H., Morikawa K. Plaque-forming cells in rabbits immunized with BCG bacilli. Infect Immun. 1972 Dec;6(6):893–898. doi: 10.1128/iai.6.6.893-898.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Parlett R. C., Chu Y. M. Immunologic response in mice and rabbits to mycobacterial antigens determined by the Jerne agar-plaque method. Am J Clin Pathol. 1967 Jun;47(6):719–728. doi: 10.1093/ajcp/47.6.719. [DOI] [PubMed] [Google Scholar]
  10. Pearlman D. S. The influence of antibodies on immunologic responses. I. The effect on the response to particulate antigen in the rabbit. J Exp Med. 1967 Jul 1;126(1):127–148. doi: 10.1084/jem.126.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sterzl J., Ríha I. Detection of cells producing 7S antibodies by the plaque technique. Nature. 1965 Nov 27;208(5013):858–859. doi: 10.1038/208858a0. [DOI] [PubMed] [Google Scholar]
  12. Wortis H. H., Taylor R. B., Dresser D. W. Antibody production studied by means of the LHG assay. I. The splenic response of CBA mice to sheep erythrocytes. Immunology. 1966 Dec;11(6):603–616. [PMC free article] [PubMed] [Google Scholar]

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