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. 1974 Dec;10(6):1445–1447. doi: 10.1128/iai.10.6.1445-1447.1974

In Vitro Tritium Labeling of Anti-Sendai Virus Antibody

Mark R Sanborn 1, Donald P Durand 1
PMCID: PMC423125  PMID: 4373399

Abstract

Anti-Sendai virus immunoglobulin G was tritium labeled in vitro by the borohydride exchange method. Tritium was incorporated into the antibody at 28.5 μCi per mg of protein, with little detectable loss of antiviral activity.

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Selected References

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  1. Abelson H. T., Smith G. H., Hoffman H. A., Rowe W. P. Use of enzyme-labeled antibody for electron microscope localization of lymphocytic choriomeningitis virus antigens in infected cell cultures. J Natl Cancer Inst. 1969 Mar;42(3):497–515. [PubMed] [Google Scholar]
  2. Cooper D., Reich E. Neurotoxin from venom of the cobra, Naja naja siamensis. Purification and radioactive labeling. J Biol Chem. 1972 May 25;247(10):3008–3013. [PubMed] [Google Scholar]
  3. Rifkin D. B., Compans R. W., Reich E. A specific labeling procedure for proteins on the outer surface of membranes. J Biol Chem. 1972 Oct 25;247(20):6432–6437. [PubMed] [Google Scholar]
  4. SALPETER M. M., BACHMANN L. AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE. A PROCEDURE FOR IMPROVING RESOLUTION, SENSITIVITY, AND CONTRAST. J Cell Biol. 1964 Aug;22:469–477. doi: 10.1083/jcb.22.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Wagner R. R., Heine J. W., Goldstein G., Schnaitman C. A. Use of antiviral-antiferritin hybrid antibody for localization of viral antigen in plasma membrane. J Virol. 1971 Feb;7(2):274–277. doi: 10.1128/jvi.7.2.274-277.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. White F. H., Riesz P. The free-radical interceptor technique as a means for the preparation of tritiated proteins. Biochem Biophys Res Commun. 1968 Feb 15;30(3):303–309. doi: 10.1016/0006-291x(68)90451-8. [DOI] [PubMed] [Google Scholar]
  7. Zull J. E., Repke D. W. Studies with tritiated polypeptide hormones. I. The preparation and properties of an active, highly tritiated derivative of parathyroid hormone: acetamidino-parathyroid hormone. J Biol Chem. 1972 Apr 10;247(7):2183–2188. [PubMed] [Google Scholar]

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