Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1995 Mar 28;92(7):2854–2858. doi: 10.1073/pnas.92.7.2854

Comprehensive allelotyping of human renal cell carcinomas using microsatellite DNA probes.

C A Thrash-Bingham 1, R E Greenberg 1, S Howard 1, A Bruzel 1, M Bremer 1, A Goll 1, H Salazar 1, J J Freed 1, K D Tartof 1
PMCID: PMC42317  PMID: 7708737

Abstract

The von Hippel-Lindau locus on chromosome 3p is a tumor suppressor gene known to be involved in nonpapillary renal cell carcinoma. A previous loss of heterozygosity (LOH) study aimed at determining the allelotype of kidney tumors has indicated that in addition to 3p, chromosome arms 5q, 6q, 10q, 11q, 17p, and 19p may also harbor tumor suppressor genes. However, cytogenetic studies reveal that chromosomes 3p, 6q, 8p, 9pq, and 14q most frequently undergo karyotypic changes in renal tumors. To resolve these differences, a collection of microsatellite DNA probes has been used to scan for LOH so that 90% of individual tumor genomes were rendered informative for allele loss. The assay is capable of detecting quantitative genomic alterations in tumor cells as well. We find that LOH is most frequent for chromosome arm 3p. However, in no tumor is 3p exclusively affected. LOH for 6q, 8p, 9pq, and 14q is also distinctly elevated for both nonpapillary as well as papillary tumors and suggest that many of the tumor suppressor loci involved may be common to the etiology of both forms of kidney cancer.

Full text

PDF
2854

Images in this article

2856Fig. 1
on p.2856
2858Fig. 2
on p.2858
2858Fig. 3
on p.2858

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cavenee W. K., Dryja T. P., Phillips R. A., Benedict W. F., Godbout R., Gallie B. L., Murphree A. L., Strong L. C., White R. L. Expression of recessive alleles by chromosomal mechanisms in retinoblastoma. 1983 Oct 27-Nov 2Nature. 305(5937):779–784. doi: 10.1038/305779a0. [DOI] [PubMed] [Google Scholar]
  2. Chuman L., Fine L. G., Cohen A. H., Saier M. H., Jr Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium. J Cell Biol. 1982 Sep;94(3):506–510. doi: 10.1083/jcb.94.3.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen A. J., Li F. P., Berg S., Marchetto D. J., Tsai S., Jacobs S. C., Brown R. S. Hereditary renal-cell carcinoma associated with a chromosomal translocation. N Engl J Med. 1979 Sep 13;301(11):592–595. doi: 10.1056/NEJM197909133011107. [DOI] [PubMed] [Google Scholar]
  4. Elfving P., Lundgren R., Cigudosa J. C., Heim S., Mandahl N., Mitelman F. Trisomy 7 in nonneoplastic kidney tissue cultured with and without epidermal growth factor. Cancer Genet Cytogenet. 1992 Nov;64(1):99–100. doi: 10.1016/0165-4608(92)90334-5. [DOI] [PubMed] [Google Scholar]
  5. Emanuel A., Szucs S., Weier H. U., Kovacs G. Clonal aberrations of chromosomes X, Y, 7 and 10 in normal kidney tissue of patients with renal cell tumors. Genes Chromosomes Cancer. 1992 Jan;4(1):75–77. doi: 10.1002/gcc.2870040110. [DOI] [PubMed] [Google Scholar]
  6. Fan Y. S., Davis L. M., Shows T. B. Mapping small DNA sequences by fluorescence in situ hybridization directly on banded metaphase chromosomes. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6223–6227. doi: 10.1073/pnas.87.16.6223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gnarra J. R., Tory K., Weng Y., Schmidt L., Wei M. H., Li H., Latif F., Liu S., Chen F., Duh F. M. Mutations of the VHL tumour suppressor gene in renal carcinoma. Nat Genet. 1994 May;7(1):85–90. doi: 10.1038/ng0594-85. [DOI] [PubMed] [Google Scholar]
  8. Gyapay G., Morissette J., Vignal A., Dib C., Fizames C., Millasseau P., Marc S., Bernardi G., Lathrop M., Weissenbach J. The 1993-94 Généthon human genetic linkage map. Nat Genet. 1994 Jun;7(2 Spec No):246–339. doi: 10.1038/ng0694supp-246. [DOI] [PubMed] [Google Scholar]
  9. Hino O., Kitagawa T., Sugano H. Relationship between serum and histochemical markers for hepatitis B virus and rate of viral integration in hepatocellular carcinomas in Japan. Int J Cancer. 1985 Jan 15;35(1):5–10. doi: 10.1002/ijc.2910350103. [DOI] [PubMed] [Google Scholar]
  10. Kovacs G., Frisch S. Clonal chromosome abnormalities in tumor cells from patients with sporadic renal cell carcinomas. Cancer Res. 1989 Feb 1;49(3):651–659. [PubMed] [Google Scholar]
  11. Kovacs G. Molecular cytogenetics of renal cell tumors. Adv Cancer Res. 1993;62:89–124. doi: 10.1016/s0065-230x(08)60316-4. [DOI] [PubMed] [Google Scholar]
  12. Kovacs G. Papillary renal cell carcinoma. A morphologic and cytogenetic study of 11 cases. Am J Pathol. 1989 Jan;134(1):27–34. [PMC free article] [PubMed] [Google Scholar]
  13. Kovacs G., Wilkens L., Papp T., de Riese W. Differentiation between papillary and nonpapillary renal cell carcinomas by DNA analysis. J Natl Cancer Inst. 1989 Apr 5;81(7):527–530. doi: 10.1093/jnci/81.7.527. [DOI] [PubMed] [Google Scholar]
  14. Latif F., Tory K., Gnarra J., Yao M., Duh F. M., Orcutt M. L., Stackhouse T., Kuzmin I., Modi W., Geil L. Identification of the von Hippel-Lindau disease tumor suppressor gene. Science. 1993 May 28;260(5112):1317–1320. doi: 10.1126/science.8493574. [DOI] [PubMed] [Google Scholar]
  15. Marx J. A challenge to p16 gene as a major tumor suppressor. Science. 1994 Jun 24;264(5167):1846–1846. doi: 10.1126/science.8009205. [DOI] [PubMed] [Google Scholar]
  16. Morita R., Ishikawa J., Tsutsumi M., Hikiji K., Tsukada Y., Kamidono S., Maeda S., Nakamura Y. Allelotype of renal cell carcinoma. Cancer Res. 1991 Feb 1;51(3):820–823. [PubMed] [Google Scholar]
  17. Pinkel D., Straume T., Gray J. W. Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci U S A. 1986 May;83(9):2934–2938. doi: 10.1073/pnas.83.9.2934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Presti J. C., Jr, Rao P. H., Chen Q., Reuter V. E., Li F. P., Fair W. R., Jhanwar S. C. Histopathological, cytogenetic, and molecular characterization of renal cortical tumors. Cancer Res. 1991 Mar 1;51(5):1544–1552. [PubMed] [Google Scholar]
  19. Shuin T., Kondo K., Torigoe S., Kishida T., Kubota Y., Hosaka M., Nagashima Y., Kitamura H., Latif F., Zbar B. Frequent somatic mutations and loss of heterozygosity of the von Hippel-Lindau tumor suppressor gene in primary human renal cell carcinomas. Cancer Res. 1994 Jun 1;54(11):2852–2855. [PubMed] [Google Scholar]
  20. Zbar B., Brauch H., Talmadge C., Linehan M. Loss of alleles of loci on the short arm of chromosome 3 in renal cell carcinoma. 1987 Jun 25-Jul 1Nature. 327(6124):721–724. doi: 10.1038/327721a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES