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. 1986 Feb;77(2):551–558. doi: 10.1172/JCI112336

Effect of protein on sympathetic nervous system activity in the rat. Evidence for nutrient-specific responses.

L N Kaufman, J B Young, L Landsberg
PMCID: PMC423378  PMID: 3753712

Abstract

Increased energy intake activates the sympathetic nervous system (SNS) in animals and man. While dietary carbohydrate and fat stimulate, the impact of dietary protein on the SNS is not well defined. The present studies examine the effect of protein ingestion on sympathetic function based upon the measurement of [3H]norepinephrine (NE) turnover in heart and interscapular brown adipose tissue (IBAT) as the index of SNS activity. In these experiments, animals were pair-fed mixtures of laboratory chow and refined preparations of casein, sucrose, and lard to permit comparisons among nutrients with total energy intake held constant or with additional energy provided in the form of a single nutrient. After 5 d of eating a 2:1 mixture of chow and either casein or sucrose cardiac, [3H]NE turnover was less (P less than 0.005) in casein-fed rats (6.4%/h and 28.9 ng NE/h) than in animals given sucrose (11.2%/h and 46.5 ng NE/h). Similar results were obtained in IBAT and in experiments using 1:1 mixtures of chow and casein/sucrose. Casein-fed animals also displayed slower rates of NE turnover than lard-fed rats in both heart (7.8%/h vs. 13.2, P less than 0.001) and IBAT (7.0%/h vs. 12.8, P less than 0.01). Addition of casein (50% increase in energy intake) to a fixed chow ration raised NE turnover slightly, but not significantly, in heart (an average increase of 15% in six experiments). Thus, in distinction to SNS activation seen with dietary carbohydrate or fat, the SNS response to dietary protein is minimal in both heart and IBAT, indicating that the effect of increased energy intake on the SNS is dependent upon diet composition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANTON A. H., SAYRE D. F. A study of the factors affecting the aluminum oxide-trihydroxyindole procedure for the analysis of catecholamines. J Pharmacol Exp Ther. 1962 Dec;138:360–375. [PubMed] [Google Scholar]
  2. Donald P., Pitts C. C., Pohl S. L. Body weight and composition in laboratory rats: effects of diets with high or low protein concentrations. Science. 1981 Jan 9;211(4478):185–186. doi: 10.1126/science.7444462. [DOI] [PubMed] [Google Scholar]
  3. Eriksson B. M., Persson B. A. Determination of catecholamines in rat heart tissue and plasma samples by liquid chromatography with electrochemical detection. J Chromatogr. 1982 Mar 12;228:143–154. doi: 10.1016/s0378-4347(00)80427-2. [DOI] [PubMed] [Google Scholar]
  4. Johnson T. S., Young J. B., Landsberg L. Sympathoadrenal responses to acute and chronic hypoxia in the rat. J Clin Invest. 1983 May;71(5):1263–1272. doi: 10.1172/JCI110876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Landsberg L., Saville M. E., Young J. B. Sympathoadrenal system and regulation of thermogenesis. Am J Physiol. 1984 Aug;247(2 Pt 1):E181–E189. doi: 10.1152/ajpendo.1984.247.2.E181. [DOI] [PubMed] [Google Scholar]
  6. Neff N. H., Tozer T. N., Hammer W., Costa E., Brodie B. B. Application of steady-state kinetics to the uptake and decline of H3-NE in the rat heart. J Pharmacol Exp Ther. 1968 Mar;160(1):48–52. [PubMed] [Google Scholar]
  7. Rowe J. W., Young J. B., Minaker K. L., Stevens A. L., Pallotta J., Landsberg L. Effect of insulin and glucose infusions on sympathetic nervous system activity in normal man. Diabetes. 1981 Mar;30(3):219–225. doi: 10.2337/diab.30.3.219. [DOI] [PubMed] [Google Scholar]
  8. Schwartz J. H., Young J. B., Landsberg L. Effect of dietary fat on sympathetic nervous system activity in the rat. J Clin Invest. 1983 Jul;72(1):361–370. doi: 10.1172/JCI110976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Vander Tuig J. G., Romsos D. R. Effects of dietary carbohydrate, fat, and protein on norepinephrine turnover in rats. Metabolism. 1984 Jan;33(1):26–33. doi: 10.1016/0026-0495(84)90158-6. [DOI] [PubMed] [Google Scholar]
  10. Wurtman R. J. Nutrients that modify brain function. Sci Am. 1982 Apr;246(4):50–59. doi: 10.1038/scientificamerican0482-50. [DOI] [PubMed] [Google Scholar]
  11. Young J. B., Fish S., Landsberg L. Sympathetic nervous system and adrenal medullary responses to ischemic injury in mice. Am J Physiol. 1983 Jul;245(1):E67–E73. doi: 10.1152/ajpendo.1983.245.1.E67. [DOI] [PubMed] [Google Scholar]
  12. Young J. B., Kaufman L. N., Saville M. E., Landsberg L. Increased sympathetic nervous system activity in rats fed a low-protein diet. Am J Physiol. 1985 May;248(5 Pt 2):R627–R637. doi: 10.1152/ajpregu.1985.248.5.R627. [DOI] [PubMed] [Google Scholar]
  13. Young J. B., Landsberg L. Effect of diet and cold exposure on norepinephrine turnover in pancreas and liver. Am J Physiol. 1979 May;236(5):E524–E533. doi: 10.1152/ajpendo.1979.236.5.E524. [DOI] [PubMed] [Google Scholar]
  14. Young J. B., Landsberg L. Stimulation of the sympathetic nervous system during sucrose feeding. Nature. 1977 Oct 13;269(5629):615–617. doi: 10.1038/269615a0. [DOI] [PubMed] [Google Scholar]
  15. Young J. B., Saville E., Rothwell N. J., Stock M. J., Landsberg L. Effect of diet and cold exposure on norepinephrine turnover in brown adipose tissue of the rat. J Clin Invest. 1982 May;69(5):1061–1071. doi: 10.1172/JCI110541. [DOI] [PMC free article] [PubMed] [Google Scholar]

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