Table 2.
Plasmids used in this study.
| Plasmid | Description | Source or reference |
|---|---|---|
| pBluescript SK+ | High-copy cloning vector; Apr | Invitrogen |
| pBKJ258 | TmS allelic-exchange vector; Emr | Lee et al. (2007) |
| pBKJ223 | I-SceI expression vector; Tcr, Apr | Janes and Stibitz (2006) |
| pRP1028 | TmS allelic-exchange vector; Spr | Dr. Scott Stibitz |
| pRP1028m | turbo-rfp-minus pRP1028; Spr | This study |
| pUC18R6KT-mini-Tn7T-Km | Apr; Kmr on mini-Tn7T; R6K replicon and oriT | Choi et al. (2005) |
| pTNS2 | Apr; R6K replicon; encodes the TnsABC+D specific transposition pathway | Choi et al. (2005) |
| pTNS3-asdEc | Helper plasmid containing asdEc for Tn7 site-specific transposition system | Kang et al. (2009) |
| pFLP2 | Apr/Cbr; Flp recombinase expression vector | Becher and Schweizer (2000) |
| pBKJΔbla1 | 2 kb flanking sequences of bla1 were cloned between NotI-SacII sites of pBKJ258; Emr | This study |
| pBKJΔbla1::pntr-gfp | Promoter pntr-driven Superfolder gfp cloned into pBKJΔbla1; Emr | This study |
| pBKJΔbla1::p0253-gfp | Promoter p0253-driven Superfolder gfp cloned into pBKJΔbla1; Emr | This study |
| pBKJΔbla1::pntr-rfp | Promoter pntr-driven TurboRed rfp cloned into pBKJΔbla1; Emr | This study |
| pBKJΔbla1::p0253-rfp | Promoter p0253-driven TurboRed rfp cloned into pBKJΔbla1; Emr | This study |
| pRP1028Δbla1::p0253-gfp | Promoter p0253-driven Superfolder GFP cloned into pRP1028; Spr | This study |
| pRP1028m−Δbla1::p0253-rfp | Promoter p0253-driven TurboRed RFP cloned into pRP1028rfp−; Spr | This study |
| pUC18R6KT-PcysZK-gfp | Promoter cysZK-driven gfp reporter fusion cloned between SmaI and ApaI sites of pUC18R6KT-mini-Tn7T-Km | This study |
| pUC18R6KT-PcysZK-rfp | Promoter cysZK-driven rfp reporter fusion cloned between SmaI and ApaI sites of pUC18R6KT-mini-Tn7T-Km | This study |
| pUC18R6KT-2PcysZK-gfp | Second copy of cysZK-driven gfp reporter fusion cloned between ApaI and KpnI sites of pUC18R6KT-PcysZK-gfp | This study |
| pUC18R6KT-2PcysZK-rfp | Second copy of cysZK-driven rfp reporter fusion cloned between ApaI and KpnI sites of pUC18R6KT-PcysZK-rfp | This study |
| pmini-Tn7-gat | mini-Tn7 integration vector based on gat | Norris et al. (2009) |
| pmini-Tn7-gat-P1-gfp | Integron promoter P1-driven egfp reporter fusion cloned between HindIII and EcoRI sites of pmini-Tn7-gat | This study |
| pmini-Tn7-gat-P1-rfp | Integron promoter P1-driven rfp reporter fusion cloned between HindIII and EcoRI sites of pmini-Tn7-gat | This study |
Kmr, kanamycin resistance; Apr, ampicillin resistance; Emr, erythromycin resistance; Cmr, chloramphenicol resistance; Spr, spectinomycin resistance.