Table 1.

Quantitative analysis of subcellular localization of GFP-VirB4 and its derivatives.

VirB4 segment	Plasmid	Total number of cells	One pole	Both poles	Others	Total number at poles	% Polar
2–789	pADI15	191	173	16	2	189	99
2–315 + 781–789	pADI15ΔR	111	9	0	102	9	0.1
236–789	pADI15ΔB	152	122	16	14	138	91
236–470	pADI18	125	66	24	35	90	72
236–444	pADI33	209	5	1	203	6	3
275–470	pADI35	129	38	8	83	46	36
562–789	pADI20	190	118	23	49	141	74
592–789	pADI27	218	153	27	38	180	83
592–721	pADI28	149	15	6	128	21	14
592–755	pADI37	190	114	16	60	130	68
643–755	pADI38	187	6	1	180	7	4
643–789	pADI39	135	70	15	50	85	63

Number of bacteria with one polar focus, two polar foci, and other phenotypes (no foci, three and more foci, and nonpolar focus) were counted. Percent polar is defined as 100× (number of bacteria with one and two polar foci/number of fluorescent bacteria).