Figure 4.

Frequency of GPI-linked protein loss progresses with age and correlates with disease phenotype. (A) Absolute number of CD24-negative reticulocytes is increased in PB of NHD13 mice compared to WT mice and increases with age. 1 × 10⁶ cells were analyzed for each sample. (B) Frequency of CD24 loss (CD24 mutants/total reticulocytes x 100) increases with age. (C) In PB of NHD13 mice CD90-negative T-cells increase with age and are increased compared to WT controls. 2 × 10⁴ events were analyzed. (D) Loss of the monocyte GPI-linked protein Ly-6C increases over disease course in NHD13 mice. 2 × 10⁴ events were analyzed.