Table 1.
Mutations in the PIG-A open reading frame of CD24-negative reticulocytes.
| Mouse | Clone | cDNA positiona (exon) | Mutation b | Consequence |
|---|---|---|---|---|
| 1 | A | 307 (exon 2) | G to A | Gly-64 to Arg |
| 802 (exon 2) | T to C | Val-229 to Ala | ||
| 1191 (exon 5) | G to A | Gly-359 to Ser | ||
| 1385 (exon 6) | T to C | Silent | ||
| 1490 (exon 6) | T to C | Silent | ||
| 1509 (exon 6) | T to C | Trp-465 to Arg | ||
| 1555 (exon 6) | A to G | Lys-480 to Arg | ||
| B | 710 (exon 2) | G to T | Silent | |
| 914 (exon 3) | deletion of A | Frameshift | ||
| 1191 (exon 5) | G to A | Gly-359 to Ser | ||
| 1531 (exon 6) | A to G | Asp-427 to Gly | ||
| C | 155 (exon 2) | T to C | Silent | |
| 767 (exon 2) | A to G | Silent | ||
| 1021 (exon 4) | A to G | Gln-302 to Arg | ||
| 1104 (exon 5) | G to C | Val-330 to Leu | ||
| 1269 (exon 6) | T to C | Phe-385 to Leu | ||
| D | 756 (exon 2) | G to A | Asp-214 to Asn | |
| 1191 (exon 5) | G to A | Gly-359 to Ser | ||
| 2 | A | 227 (exon 2) | G to A | Met-37 to Ile |
| 235 (exon 2) | A to G | Asp-40 to Gly | ||
| 722 (exon 2) | A to G | Ile-202 to Met | ||
| 742 (exon 2) | C to T | Ala-209 to Val | ||
| 1270 (exon 5) | T to C | Phe-385 to Ser | ||
| B | 328 (exon 2) | C to T | Thr-71 to Ile | |
| 527 (exon2) | deletion of T | Frameshift | ||
| 992 (exon 4) | A to G | Silent | ||
| 1253 (exon 5) | C to t | Silent | ||
| C | 537 (exon 2) | C to T | Silent | |
| 542 (exon 2) | C to T | His-141 to Tyr | ||
| D | 1389 (exon 6) | A to G | Thr-425 to Ala | |
| 3 | A | 301 (exon 2) | A to G | Glu-62 to Gly |
| 571 (exon 2) | C to T | Thr-152 to Ile | ||
| 660 (exon 2) | A to G | Ile-182 to Val | ||
| 1335 (exon 6) | A to G | Silent | ||
| B | 301 (exon 2) | A to G | Glu-62 to Gly | |
| 571 (exon 2) | C to T | Thr-152 to Ile | ||
| 660 (exon 2) | A to G | Ile-182 to Val | ||
| 1335 (exon 6) | A to G | Silent | ||
| C | 301 (exon 2) | A to G | Glu-62 to Gly | |
| 571 (exon 2) | C to T | Thr-152 to Ile | ||
| 660 (exon 2) | A to G | Ile-182 to Val | ||
| 1335 (exon 6) | A to G | Silent | ||
| D | 301 (exon 2) | A to G | Glu-62 to Gly | |
| 571 (exon 2) | C to T | Thr-152 to Ile | ||
| 660 (exon 2) | A to G | Ile-182 to Val | ||
| 1335 (exon 6) | A to G | Silent |
a
The nucleotide position is based on the numbering of the mouse PIG-A cDNA sequence (Genbank NM_011081).
b
Sequence alterations in the nontranscribed cDNA strand compared to WT PIG-A.