Table 1.
Pathogen (targeted gene) | Collection Visit | Ɏ * Mean copies/uL | * Positive (%, n = 34) |
---|---|---|---|
Staphylococcus aureus (nuc) | 1 | 951.3 | 41.2 |
2 | 6,623.1 | 41.2 | |
3 | 351.4 | 52.9 | |
Enterococcus spp. (16S) | 1 | 1,702.9 | 52.9 |
2 | 2,877.1 | 70.6 | |
3 | 1,823.6 | 61.8 | |
Candida albicans (18S) | 1 | 663.8 | 8.8 |
2 | 336.3 | 5.9 | |
3 | 142.7 | 2.9 | |
MRSA (mecA|orfX) | 1 | 173.5 | 2.9 |
2 | 1,405.8 | 5.9 | |
3 | 3,763.5 | 2.9 |
Ɏ nuc (~1 copy/cell); Enterococcus (~5 16S rRNA copies/cell); C. albicans (~100 copies/cell).
* qPCR cut-off of 100 copies/ul defined as qPCR limit of detection used to identify a HCW as positive.