Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1985 Mar;75(3):874–882. doi: 10.1172/JCI111786

Effects of postprandial lipemia on plasma cholesterol metabolism.

G R Castro, C J Fielding
PMCID: PMC423618  PMID: 3856571

Abstract

Cholesterol net transport, esterification, and cholesteryl ester transfer have been determined in plasma during fasting, and postprandially, after a high fat-cholesterol meal. Significant rises in plasma triglyceride, phospholipid, and free cholesterol were associated with increases in cholesterol net transport, esterification, and transfer (all P less than 0.005), which were well correlated in individual subjects (r greater than 0.60). Essentially, the whole of free cholesterol required for such increased esterification was derived from cell membranes, when cultured fibroblasts were present, despite the increased level of free cholesterol in postprandial plasma; most of the additional cholesteryl ester generated was transferred to the low and very low density lipoproteins (LDL and VLDL) of plasma. Postprandial LDL (the major carrier of free and ester cholesterol and phospholipids among the acceptor lipoproteins) contained significantly decreased ratios of free cholesterol to phospholipid (P less than 0.001), which may modulate the increased transfer of cholesteryl ester to VLDL and LDL. These data suggest that the presence of postprandial acceptor lipoproteins in plasma may play an important role in stimulating the "reverse" transport of cholesterol from peripheral cells for hepatic degradation, which is effective even after the ingestion of dietary cholesterol.

Full text

PDF
874

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aron L., Jones S., Fielding C. J. Human plasma lecithin-cholesterol acyltransferase. Characterization of cofactor-dependent phospholipase activity. J Biol Chem. 1978 Oct 25;253(20):7220–7226. [PubMed] [Google Scholar]
  2. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  3. Blum C. B. Dynamics of apolipoprotein E metabolism in humans. J Lipid Res. 1982 Dec;23(9):1308–1316. [PubMed] [Google Scholar]
  4. CARLSON L. A. DETERMINATION OF SERUM TRIGLYCERIDES. J Atheroscler Res. 1963 Jul-Aug;3:334–336. doi: 10.1016/s0368-1319(63)80012-5. [DOI] [PubMed] [Google Scholar]
  5. Castro G. R., Fielding C. J. Evidence for the distribution of apolipoprotein E between lipoprotein classes in human normocholesterolemic plasma and for the origin of unassociated apolipoprotein E (Lp-E). J Lipid Res. 1984 Jan;25(1):58–67. [PubMed] [Google Scholar]
  6. Chajek T., Aron L., Fielding C. J. Interaction of lecithin:cholesterol acyltransferase and cholesteryl ester transfer protein in the transport of cholesteryl ester into sphingomyelin liposomes. Biochemistry. 1980 Aug 5;19(16):3673–3677. doi: 10.1021/bi00557a006. [DOI] [PubMed] [Google Scholar]
  7. Chajek T., Fielding C. J. Isolation and characterization of a human serum cholesteryl ester transfer protein. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3445–3449. doi: 10.1073/pnas.75.7.3445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fielding C. J., Fielding P. E. Cholesterol transport between cells and body fluids. Role of plasma lipoproteins and the plasma cholesterol esterification system. Med Clin North Am. 1982 Mar;66(2):363–373. doi: 10.1016/s0025-7125(16)31425-0. [DOI] [PubMed] [Google Scholar]
  9. Fielding C. J., Fielding P. E. Evidence for a lipoprotein carrier in human plasma catalyzing sterol efflux from cultured fibroblasts and its relationship to lecithin:cholesterol acyltransferase. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3911–3914. doi: 10.1073/pnas.78.6.3911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fielding C. J., Fielding P. E. Regulation of human plasma lecithin:cholesterol acyltransferase activity by lipoprotein acceptor cholesteryl ester content. J Biol Chem. 1981 Mar 10;256(5):2102–2104. [PubMed] [Google Scholar]
  11. Fielding C. J. Lecithin-cholesterol acyltransferase and cholesterol transport. Methods Enzymol. 1985;111:267–274. doi: 10.1016/s0076-6879(85)11014-1. [DOI] [PubMed] [Google Scholar]
  12. Fielding C. J., Reaven G. M., Fielding P. E. Human noninsulin-dependent diabetes: identification of a defect in plasma cholesterol transport normalized in vivo by insulin and in vitro by selective immunoadsorption of apolipoprotein E. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6365–6369. doi: 10.1073/pnas.79.20.6365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fielding C. J., Reaven G. M., Liu G., Fielding P. E. Increased free cholesterol in plasma low and very low density lipoproteins in non-insulin-dependent diabetes mellitus: its role in the inhibition of cholesteryl ester transfer. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2512–2516. doi: 10.1073/pnas.81.8.2512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Fielding P. E., Davison P. M., Karasek M. A., Fielding C. J. Regulation of sterol transport in human microvascular endothelial cells. J Cell Biol. 1982 Aug;94(2):350–354. doi: 10.1083/jcb.94.2.350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Fielding P. E., Fielding C. J., Havel R. J., Kane J. P., Tun P. Cholesterol net transport, esterification, and transfer in human hyperlipidemic plasma. J Clin Invest. 1983 Mar;71(3):449–460. doi: 10.1172/JCI110789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ihm J., Quinn D. M., Busch S. J., Chataing B., Harmony J. A. Kinetics of plasma protein-catalyzed exchange of phosphatidylcholine and cholesteryl ester between plasma lipoproteins. J Lipid Res. 1982 Dec;23(9):1328–1341. [PubMed] [Google Scholar]
  17. Kuksis A., Myher J. J., Geher K., Jones G. J., Breckenridge W. C., Feather T., Hewitt D., Little J. A. Decreased plasma phosphatidylcholine/free cholesterol ratio as an indicator of risk for ischemic vascular disease. Arteriosclerosis. 1982 Jul-Aug;2(4):296–302. doi: 10.1161/01.atv.2.4.296. [DOI] [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Marcel Y. L., Vezina C. Lecithin: cholesterol acyltransferase of human plasma. Role of chylomicrons, very low, and high density lipoproteins in the reaction. J Biol Chem. 1973 Dec 10;248(23):8254–8259. [PubMed] [Google Scholar]
  20. Miller K. W., Small D. M. Surface-to-core and interparticle equilibrium distributions of triglyceride-rich lipoprotein lipids. J Biol Chem. 1983 Nov 25;258(22):13772–13784. [PubMed] [Google Scholar]
  21. Pagnan A., Havel R. J., Kane J. P., Kotite L. Characterization of human very low density lipoproteins containing two electrophoretic populations: double pre-beta lipoproteinemia and primary dysbetalipoproteinemia. J Lipid Res. 1977 Sep;18(5):613–622. [PubMed] [Google Scholar]
  22. REHNBORG C. S., NICHOLS A. V. THE FATE OF CHOLESTERYL ESTERS IN HUMAN SERUM INCUBATED IN VITRO AT 38 DEGREES. Biochim Biophys Acta. 1964 Oct 2;84:596–603. doi: 10.1016/0926-6542(64)90129-5. [DOI] [PubMed] [Google Scholar]
  23. Rose H. G., Juliano J. Regulation of plasma lecithin:cholesterol acyltransferase. II. Activation during alimentary lipemia. J Lab Clin Med. 1977 Mar;89(3):525–532. [PubMed] [Google Scholar]
  24. Vakakis N., Redgrave T. G., Small D. M., Castelli W. P. Cholesterol content of red blood cells and low-density lipoproteins in hypertriglyceridemia. Biochim Biophys Acta. 1983 May 16;751(3):280–285. doi: 10.1016/0005-2760(83)90285-0. [DOI] [PubMed] [Google Scholar]
  25. Vigne J. L., Havel R. J. Metabolism of apolipoprotein A-I of chylomicrons in rats and humans. Can J Biochem. 1981 Aug;59(8):613–618. doi: 10.1139/o81-085. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES