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. 1986 Sep;78(3):637–642. doi: 10.1172/JCI112621

Bone deficit in ovariectomized rats. Functional contribution of the marrow stromal cell population and the effect of oral dihydrotachysterol treatment.

C Tabuchi, D J Simmons, A Fausto, J E Russell, I Binderman, L V Avioli
PMCID: PMC423638  PMID: 3528218

Abstract

This study investigates the proliferative and osteogenic role of marrow stromal/osteoprogenitor cells in the development of the cortical bone deficit in ovariectomized (OVX) female rats. In vitro, clonal growth of marrow stromal cells from OVX rats was significantly impaired (vs. sham-operated controls). Yet in vivo, cells from sham-operated and OVX rats had equal osteogenic potential in several in vivo experimental situations, such as in intraperitoneally implanted millipore diffusion chambers and in intramuscular implants of marrow plus osteoinductive bone matrix (composite grafts). Long-term (6 mo) dihydrotachysterol (DHT) treatment of OVX rats enhanced their in vitro proliferative potential and clonal growth, as well as their osteogenic expression in composite grafts. The observation that the in vivo osteogenic performance of OVX rat marrow stromal cells was normal at extraosseous sites suggests that the mechanisms leading to osteopenia may involve an abnormality in cell-matrix interactions.

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Selected References

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  1. Aitken J. M., Armstrong E., Anderson J. B. Osteoporosis after oophorectomy in the mature female rat and the effect of oestrogen and-or progestogen replacement therapy in its prevention. J Endocrinol. 1972 Oct;55(1):79–87. doi: 10.1677/joe.0.0550079. [DOI] [PubMed] [Google Scholar]
  2. Ashton B. A., Allen T. D., Howlett C. R., Eaglesom C. C., Hattori A., Owen M. Formation of bone and cartilage by marrow stromal cells in diffusion chambers in vivo. Clin Orthop Relat Res. 1980 Sep;(151):294–307. [PubMed] [Google Scholar]
  3. Ashton B. A., Eaglesom C. C., Bab I., Owen M. E. Distribution of fibroblastic colony-forming cells in rabbit bone marrow and assay of their osteogenic potential by an in vivo diffusion chamber method. Calcif Tissue Int. 1984 Jan;36(1):83–86. doi: 10.1007/BF02405298. [DOI] [PubMed] [Google Scholar]
  4. BARNETT E., NORDIN B. E. The radiological diagnosis of osteoporosis: a new approach. Clin Radiol. 1960 Jul;11:166–174. doi: 10.1016/s0009-9260(60)80012-8. [DOI] [PubMed] [Google Scholar]
  5. Baxter J. D. Mechanisms of glucocorticoid inhibition of growth. Kidney Int. 1978 Oct;14(4):330–333. doi: 10.1038/ki.1978.132. [DOI] [PubMed] [Google Scholar]
  6. Bell N. H. Vitamin D-endocrine system. J Clin Invest. 1985 Jul;76(1):1–6. doi: 10.1172/JCI111930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Canalis E., Centrella M., Urist M. R. Effect of partially purified bone morphogenetic protein on DNA synthesis and cell replication in calvarial and fibroblast cultures. Clin Orthop Relat Res. 1985 Sep;(198):289–296. [PubMed] [Google Scholar]
  8. Friedenstein A. J. Precursor cells of mechanocytes. Int Rev Cytol. 1976;47:327–359. doi: 10.1016/s0074-7696(08)60092-3. [DOI] [PubMed] [Google Scholar]
  9. Haas H. G., Dambacher M. A., Guncaga J., Lauffenburger T., Lämmle B., Olah J. 1,25(OH)2 vitamin D3 in osteoporosis--a pilot study. Horm Metab Res. 1979 Feb;11(2):168–171. doi: 10.1055/s-0028-1092703. [DOI] [PubMed] [Google Scholar]
  10. Jensen G. F., Christiansen C., Transbøl I. Treatment of post menopausal osteoporosis. A controlled therapeutic trial comparing oestrogen/gestagen, 1,25-dihydroxy-vitamin D3 and calcium. Clin Endocrinol (Oxf) 1982 May;16(5):515–524. doi: 10.1111/j.1365-2265.1982.tb02769.x. [DOI] [PubMed] [Google Scholar]
  11. Kurihara N., Ikeda K., Hakeda Y., Tsunoi M., Maeda N., Kumegawa M. Effect of 1,25-dihydroxyvitamin D3 on alkaline phosphatase activity and collagen synthesis in osteoblastic cells, clone MC3T3-E1. Biochem Biophys Res Commun. 1984 Mar 15;119(2):767–771. doi: 10.1016/s0006-291x(84)80316-2. [DOI] [PubMed] [Google Scholar]
  12. Lindgren J. U., Lindholm T. S. Effect of 1-alpha-hydroxyvitamin D3 on osteoporosis in rats induced by oophorectomy. Calcif Tissue Int. 1979 Apr 17;27(2):161–164. doi: 10.1007/BF02441179. [DOI] [PubMed] [Google Scholar]
  13. Lindgren U., DeLuca H. F. Role of parathyroid hormone and 1,25-dihydroxyvitamin D3 in the development of osteopenia in oophorectomized rats. Calcif Tissue Int. 1982 Sep;34(5):510–514. doi: 10.1007/BF02411294. [DOI] [PubMed] [Google Scholar]
  14. Lindholm T. S., Sevastikoglou J. A., Lindgren U. Treatment of patients with senile, post-menopausal and corticosteroid-induced osteoporosis with 1alpha-hydroxyvitamin D3 and calcium: short- and long-term effects. Clin Endocrinol (Oxf) 1977 Dec;7 (Suppl):183s–189s. doi: 10.1111/j.1365-2265.1977.tb03380.x. [DOI] [PubMed] [Google Scholar]
  15. Meisami E. Complete recovery of growth deficits after reversal of PTU-induced postnatal hypothyroidism in the female rat: a model for catch-up growth. Life Sci. 1984 Apr 9;34(15):1487–1496. doi: 10.1016/0024-3205(84)90064-x. [DOI] [PubMed] [Google Scholar]
  16. Osdoby P., Caplan A. I. Osteogenesis in cultures of limb mesenchymal cells. Dev Biol. 1979 Nov;73(1):84–102. doi: 10.1016/0012-1606(79)90140-4. [DOI] [PubMed] [Google Scholar]
  17. Price P. A., Urist M. R., Otawara Y. Matrix Gla protein, a new gamma-carboxyglutamic acid-containing protein which is associated with the organic matrix of bone. Biochem Biophys Res Commun. 1983 Dec 28;117(3):765–771. doi: 10.1016/0006-291x(83)91663-7. [DOI] [PubMed] [Google Scholar]
  18. Saville P. D. Changes in skeletal mass and fragility with castration in the rat; a model of osteoporosis. J Am Geriatr Soc. 1969 Feb;17(2):155–166. doi: 10.1111/j.1532-5415.1969.tb03169.x. [DOI] [PubMed] [Google Scholar]
  19. Silbermann M., Levitan S., Kleinhaus U., Finkelbrand S. Long bone growth during prolonged intermittent corticosteroid treatment and subsequent rehabilitation. Cell Tissue Res. 1979 Sep 2;201(1):51–62. doi: 10.1007/BF00238047. [DOI] [PubMed] [Google Scholar]
  20. Tibone K. W., Bernard G. W. A new in vitro model of intramembranous osteogenesis from adult bone marrow stem cells. Prog Clin Biol Res. 1982;101:107–123. [PubMed] [Google Scholar]
  21. Urist M. R., DeLange R. J., Finerman G. A. Bone cell differentiation and growth factors. Science. 1983 May 13;220(4598):680–686. doi: 10.1126/science.6403986. [DOI] [PubMed] [Google Scholar]
  22. Urist M. R., Silverman B. F., Büring K., Dubuc F. L., Rosenberg J. M. The bone induction principle. Clin Orthop Relat Res. 1967 Jul-Aug;53:243–283. [PubMed] [Google Scholar]
  23. Villanueva A., Frost H., Ilnicki L., Frame B., Smith R., Arnstein R. Cortical bone dynamics measured by means of tetracycline labeling in 21 cases of osteoporosis. J Lab Clin Med. 1966 Oct;68(4):599–616. [PubMed] [Google Scholar]
  24. Vukicević S., Stavljenić A., Bagi C., Vujicić G., Kracun I., Winter I. 1a,25-Dihydroxyvitamin D3 stimulates alkaline phosphatase activity and inhibits soft-tissue proliferation in implants of bone matrix. Clin Orthop Relat Res. 1985 Jun;(196):285–291. [PubMed] [Google Scholar]

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