Abstract
Bone marrow cells from a patient with Ph' positive chronic myelogenous leukemia in chronic phase were cultured for multilineage hematopoietic colonies (CFU-GEMMT), erythroid bursts, and granulocytic colonies. With CFU-GEMMT colonies, T lymphocytes were identified by reaction with monoclonal antibodies Leu-5 and OKT-3; B cells were identified by reaction with B1. All CFU-GEMMT colonies examined contained the Ph' chromosome. Recloned secondary colonies of T cells reacted with Leu-5 and OKT-3 and were Ph' positive. This demonstrates that Ph' positive T lymphocytes were generated from the pluripotential stem cell of this patient. The presence of B cells in the mixed colonies indicates that these may also be derived from the neoplastic clone.
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- Bakhshi A., Minowada J., Arnold A., Cossman J., Jensen J. P., Whang-Peng J., Waldmann T. A., Korsmeyer S. J. Lymphoid blast crises of chronic myelogenous leukemia represent stages in the development of B-cell precursors. N Engl J Med. 1983 Oct 6;309(14):826–831. doi: 10.1056/NEJM198310063091404. [DOI] [PubMed] [Google Scholar]
- Bross K. J., Schmidt G. M., Blume K. G., Spruce W. E., Farbstein M. J. Confirmation of bone marrow engraftment by demonstration of blood group antigens on red blood cell ghosts. Transplantation. 1979 Sep;28(3):257–259. doi: 10.1097/00007890-197909000-00021. [DOI] [PubMed] [Google Scholar]
- Dubé I. D., Eaves C. J., Kalousek D. K., Eaves A. C. A method for obtaining high quality chromosome preparations from single hemopoietic colonies on a routine basis. Cancer Genet Cytogenet. 1981 Oct;4(2):157–168. doi: 10.1016/0165-4608(81)90080-7. [DOI] [PubMed] [Google Scholar]
- Dubé I. D., Gupta C. M., Kalousek D. K., Eaves C. J., Eaves A. C. Cytogenetic studies of early myeloid progenitor compartments in Ph1-positive chronic myeloid leukaemia (CML). I. Persistence of Ph1-negative committed progenitors that are suppressed from differentiating in vivo. Br J Haematol. 1984 Apr;56(4):633–644. doi: 10.1111/j.1365-2141.1984.tb02187.x. [DOI] [PubMed] [Google Scholar]
- Fauser A. A., Löhr G. W. Recloned colonies positive for T-cell-associated antigens derived from mixed hemopoietic colonies (CFU-GEMM)1. Proc Soc Exp Biol Med. 1982 Jun;170(2):220–224. doi: 10.3181/00379727-170-41422. [DOI] [PubMed] [Google Scholar]
- Fauser A. A., Messner H. A. Granuloerythropoietic colonies in human bone marrow, peripheral blood, and cord blood. Blood. 1978 Dec;52(6):1243–1248. [PubMed] [Google Scholar]
- Fauser A. A., Messner H. A. Identification of megakaryocytes, macrophages, and eosinophils in colonies of human bone marrow containing neurtophilic granulocytes and erythroblasts. Blood. 1979 May;53(5):1023–1027. [PubMed] [Google Scholar]
- Fauser A. A., Neumann H. A., Bross K. G., Kanz L., Löhr G. W. Cytotoxic T-cell clones derived from pluripotent stem cells (CFU-GEMM) of patients with Hodgkin's lymphoma. Blood. 1982 Dec;60(6):1317–1320. [PubMed] [Google Scholar]
- Fialkow P. J., Jacobson R. J., Papayannopoulou T. Chronic myelocytic leukemia: clonal origin in a stem cell common to the granulocyte, erythrocyte, platelet and monocyte/macrophage. Am J Med. 1977 Jul;63(1):125–130. doi: 10.1016/0002-9343(77)90124-3. [DOI] [PubMed] [Google Scholar]
- Messner H. A., Izaquirre C. A., Jamal N. Identification of T lymphocytes in human mixed hemopoietic colonies. Blood. 1981 Aug;58(2):402–405. [PubMed] [Google Scholar]
- Powell J. S., Fialkow P. J., Adamson J. W. Polycythemia vera: studies of hemopoiesis in continuous long-term culture of human marrow. J Cell Physiol Suppl. 1982;1:79–85. doi: 10.1002/jcp.1041130413. [DOI] [PubMed] [Google Scholar]