Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1986 Oct;78(4):922–927. doi: 10.1172/JCI112681

In vitro myotrophic effect of serum kappa chain immunoglobulins from a patient with kappa light chain myeloma and muscular hypertrophy.

C Delaporte, B Varet, M Fardeau, D Nochy, A Ract
PMCID: PMC423719  PMID: 3093534

Abstract

Muscle hypertrophy due to enlarged muscle fibers was accompanied by kappa light chain myeloma in a 62-yr-old man. Immunofluorescence showed kappa light chain deposits around muscle fibers. We hypothesized that a circulating growth factor may be involved in the pathogeny of this muscular hypertrophy. Patient serum cultured with muscle cells showed that (a) the patient's serum exhibited a trophic effect on human muscle cells in culture, (b) this trophic effect increased the differentiation and did not influence the proliferation of human muscle cells, and (c) the fraction of the patient's serum immunoadsorbed on antihuman kappa chain antibodies exhibited the same in vitro effect on the muscle cells, whereas the fraction immunoadsorbed on antihuman lambda chain antibodies did not. These results support the hypothesis that the patient's kappa light chains have a specific enhancing effect on human muscle cell differentiation, perhaps leading to an acquired muscular hypertrophy.

Full text

PDF
922

Images in this article

923Image
on p.923
924Image
on p.924
924Image
on p.924
924Image
on p.924
924Image
on p.924
925Image
on p.925
925Image
on p.925
926Image
on p.926
926Image
on p.926

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Delaporte C., Dehaupas M., Fardeau M. Comparison between the growth pattern of cell cultures from normal and Duchenne dystrophy muscle. J Neurol Sci. 1984 May;64(2):149–160. doi: 10.1016/0022-510x(84)90033-9. [DOI] [PubMed] [Google Scholar]
  2. Delaporte C., Fardeau M. Effets du sérum d'un malade ayant un myélome et une hypertrophie musculaire diffuse sur la croissance des cellules musculaires humaines en culture. C R Acad Sci III. 1984;298(3):49–54. [PubMed] [Google Scholar]
  3. Delaporte C., Gros F., Jonsson C., Bergström J. In vitro cytotoxic properties of plasma samples from uremic patients. Clin Nephrol. 1982 May;17(5):247–253. [PubMed] [Google Scholar]
  4. Ewton D. Z., Florini J. R. Relative effects of the somatomedins, multiplication-stimulating activity, and growth hormone on myoblasts and myotubes in culture. Endocrinology. 1980 Feb;106(2):577–583. doi: 10.1210/endo-106-2-577. [DOI] [PubMed] [Google Scholar]
  5. FISHER J. H. Primary amyloidosis. Can Med Assoc J. 1958 Feb 15;78(4):264–266. [PMC free article] [PubMed] [Google Scholar]
  6. Ii I., Kimura I., Ozawa E. A myotrophic protein from chick embryo extract: its purification, identity to transferrin, and indispensability for avian myogenesis. Dev Biol. 1982 Dec;94(2):366–377. doi: 10.1016/0012-1606(82)90354-2. [DOI] [PubMed] [Google Scholar]
  7. Kahn A., Cottreau D., Daegelen D., Dreyfus J. C. Cell-free translation of messenger RNAs from adult and fetal human muscle. Characterization of neosynthesized glycogen phosphorylase, phosphofructokinase and glucose phosphate isomerase. Eur J Biochem. 1981 May;116(1):7–12. doi: 10.1111/j.1432-1033.1981.tb05293.x. [DOI] [PubMed] [Google Scholar]
  8. Kumegawa M., Ikeda E., Hosoda S., Takuma T. In vitro effects of thyroxine and insulin on myoblasts from chick embryo skeletal muscle. Dev Biol. 1980 Oct;79(2):493–499. doi: 10.1016/0012-1606(80)90134-7. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lange R. K. Primary amyloidosis of muscle. South Med J. 1970 Mar;63(3):321–323. doi: 10.1097/00007611-197003000-00018. [DOI] [PubMed] [Google Scholar]
  11. Linder J., Croker B. P., Vollmer R. T., Shelburne J. Systemic kappa light-chain deposition. An ultrastructural and immunohistochemical study. Am J Surg Pathol. 1983 Jan;7(1):85–93. doi: 10.1097/00000478-198301000-00009. [DOI] [PubMed] [Google Scholar]
  12. Linkhart T. A., Clegg C. H., Hauschika S. D. Myogenic differentiation in permanent clonal mouse myoblast cell lines: regulation by macromolecular growth factors in the culture medium. Dev Biol. 1981 Aug;86(1):19–30. doi: 10.1016/0012-1606(81)90311-0. [DOI] [PubMed] [Google Scholar]
  13. Martin J. J., Van Bogaert L., Van Damme J., Peremans J. Sur une pseudo-myopathie ligneuse généralisée par amyloïdose primaire endomysio-vasculaire. J Neurol Sci. 1970 Aug;11(2):147–166. doi: 10.1016/0022-510x(70)90125-5. [DOI] [PubMed] [Google Scholar]
  14. Preud'homme J. L., Morel-Maroger L., Brouet J. C., Cerf M., Mignon F., Guglielmi P., Seligmann M. Synthesis of abnormal immunoglobulins in lymphoplasmacytic disorders with visceral light chain deposition. Am J Med. 1980 Nov;69(5):703–710. doi: 10.1016/0002-9343(80)90421-0. [DOI] [PubMed] [Google Scholar]
  15. Randall R. E., Williamson W. C., Jr, Mullinax F., Tung M. Y., Still W. J. Manifestations of systemic light chain deposition. Am J Med. 1976 Feb;60(2):293–299. doi: 10.1016/0002-9343(76)90440-x. [DOI] [PubMed] [Google Scholar]
  16. Ringel S. P., Claman H. N. Amyloid-associated muscle pseudohypertrophy. Arch Neurol. 1982 Jul;39(7):413–417. doi: 10.1001/archneur.1982.00510190031009. [DOI] [PubMed] [Google Scholar]
  17. Tubbs R. R., Gephardt G. N., McMahon J. T., Hall P. M., Valenzuela R., Vidt D. G. Light chain nephropathy. Am J Med. 1981 Aug;71(2):263–269. doi: 10.1016/0002-9343(81)90127-3. [DOI] [PubMed] [Google Scholar]
  18. Verret J. M., Lapresle J. L'hypertrophie musculaire en clinique neurologique. Acta Neurol Belg. 1981 Jan-Feb;81(1):33–51. [PubMed] [Google Scholar]
  19. Whitaker J. N., Hashimoto K., Quinones M. Skeletal muscle pseudohypertrophy in primary amyloidosis. Neurology. 1977 Jan;27(1):47–54. doi: 10.1212/wnl.27.1.47. [DOI] [PubMed] [Google Scholar]
  20. White N. K., Bonner P. H., Nelson D. R., Hauschka S. D. Clonal analysis of vertebrate myogenesis. IV. Medium-dependent classification of colony-forming cells. Dev Biol. 1975 Jun;44(2):346–361. doi: 10.1016/0012-1606(75)90405-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES