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. 1986 Oct;78(4):983–988. doi: 10.1172/JCI112689

Immune responses during human Schistosomiasis mansoni. Evidence for antiidiotypic T lymphocyte responsiveness.

M S Lima, G Gazzinelli, E Nascimento, J C Parra, M A Montesano, D G Colley
PMCID: PMC423737  PMID: 3093535

Abstract

We present a method for the examination of antiidiotypic cell-mediated reactivity during chronic human infections. Pooled and individual sera from patients with schistosomiasis mansoni were purified on immunoaffinity columns of schistosomal egg antigens (SEA). The eluates contained anti-SEA antibodies, but not SEA. These antibody preparations, and their F(ab)2 fragments, stimulated dose-dependent proliferation of peripheral blood mononuclear cells (PBMN) and T lymphocytes from some, but not all active or former schistosomiasis mansoni patients, and could do so autologously. Stimulation required presentation by plastic-adherent cells. The eluates did not stimulate PBMN from persons who had never had schistosomiasis. Affinity-purified anti-SEA antibodies from former patients (cured for greater than 10 yr) did not stimulate PBMN from patients with active infections. Reabsorption on SEA columns removed stimulatory activity from the eluates. We propose that multiclonal, SEA-related idiotypes expressed by some anti-SEA antibodies stimulate proliferation of T lymphocytes that express antiidiotypic specificities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe T., Colley D. G. Modulation of Schistosoma mansoni egg-induced granuloma formation. III. Evidence for an anti-idiotypic, I-J-positive, I-J-restricted, soluble T suppressor factor. J Immunol. 1984 Apr;132(4):2084–2088. [PubMed] [Google Scholar]
  2. Boros D. L., Warren K. S. Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated from Schistosoma mansoni eggs. J Exp Med. 1970 Sep 1;132(3):488–507. doi: 10.1084/jem.132.3.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chensue S. W., Boros D. L. Modulation of granulomatous hypersensitivity. I. Characterization of T lymphocytes involved in the adoptive suppression of granuloma formation in Schistosoma mansoni-infected mice. J Immunol. 1979 Sep;123(3):1409–1414. [PubMed] [Google Scholar]
  4. Chensue S. W., Wellhausen S. R., Boros D. L. Modulation of granulomatous hypersensitivity. II. Participation of Ly 1+ and Ly 2+ T lymphocytes in the suppression of granuloma formation and lymphokine production in Schistosoma mansoni-infected mice. J Immunol. 1981 Jul;127(1):363–367. [PubMed] [Google Scholar]
  5. Colley D. G. Adoptive suppression of granuloma formation. J Exp Med. 1976 Mar 1;143(3):696–700. doi: 10.1084/jem.143.3.696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Colley D. G., Cook J. A., Freeman G. L., Jr, Bartholomew R. K., Jordan P. Immune responses during human schistosomiasis mansoni. I. In vitro lymphocyte blastogenic responses to heterogeneous antigenic preparations from schistosome eggs, worms and cercariae. Int Arch Allergy Appl Immunol. 1977;53(5):420–433. [PubMed] [Google Scholar]
  7. Doughty B. L., Ottesen E. A., Nash T. E., Phillips S. M. Delayed hypersensitivity granuloma formation around Schistosoma mansoni eggs in vitro. III. Granuloma formation and modulation in human Schistosomiasis mansoni. J Immunol. 1984 Aug;133(2):993–997. [PubMed] [Google Scholar]
  8. Gazzinelli G., Katz N., Rocha R. S., Colley D. G. Immune responses during human Schistosomiasis mansoni. VIII. Differential in vitro cellular responsiveness to adult worm and schistosomular tegumental preparations. Am J Trop Med Hyg. 1983 Mar;32(2):326–333. [PubMed] [Google Scholar]
  9. Gazzinelli G., Katz N., Rocha R. S., Colley D. G. Immune responses during human schistosomiasis mansoni. X. Production and standardization of an antigen-induced mitogenic activity by peripheral blood mononuclear cells from treated, but not active cases of schistosomiasis. J Immunol. 1983 Jun;130(6):2891–2895. [PubMed] [Google Scholar]
  10. Gazzinelli G., Lambertucci J. R., Katz N., Rocha R. S., Lima M. S., Colley D. G. Immune responses during human Schistosomiasis mansoni. XI. Immunologic status of patients with acute infections and after treatment. J Immunol. 1985 Sep;135(3):2121–2127. [PubMed] [Google Scholar]
  11. Green W. F., Colley D. G. Modulation of Schistosoma mansoni egg-induced granuloma formation: I-J restriction of T cell-mediated suppression in a chronic parasitic infection. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1152–1156. doi: 10.1073/pnas.78.2.1152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grzych J. M., Capron M., Lambert P. H., Dissous C., Torres S., Capron A. An anti-idiotype vaccine against experimental schistosomiasis. Nature. 1985 Jul 4;316(6023):74–76. doi: 10.1038/316074a0. [DOI] [PubMed] [Google Scholar]
  13. Jerne N. K. Towards a network theory of the immune system. Ann Immunol (Paris) 1974 Jan;125C(1-2):373–389. [PubMed] [Google Scholar]
  14. Katz N., Chaves A., Pellegrino J. A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo. 1972 Nov-Dec;14(6):397–400. [PubMed] [Google Scholar]
  15. Kresina T. F., Nisonoff A. Passive transfer of the idiotypically suppressed state by serum from suppressed mice and transfer of suppression from mothers to offspring. J Exp Med. 1983 Jan 1;157(1):15–23. doi: 10.1084/jem.157.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. McNamara M. K., Ward R. E., Kohler H. Monoclonal idiotope vaccine against Streptococcus pneumoniae infection. Science. 1984 Dec 14;226(4680):1325–1326. doi: 10.1126/science.6505692. [DOI] [PubMed] [Google Scholar]
  19. Nash T. E., Cheever A. W., Ottesen E. A., Cook J. A. Schistosome infections in humans: perspectives and recent findings. NIH conference. Ann Intern Med. 1982 Nov;97(5):740–754. doi: 10.7326/0003-4819-97-5-740. [DOI] [PubMed] [Google Scholar]
  20. Olds G. R., Kresina T. F. Network interactions in Schistosoma japonicum infection. Identification and characterization of a serologically distinct immunoregulatory auto-antiidiotypic antibody population. J Clin Invest. 1985 Dec;76(6):2338–2347. doi: 10.1172/JCI112245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Powell M. R., Colley D. G. Demonstration of splenic auto-anti-idiotypic plaque-forming cells in mice infected with Schistosoma mansoni. J Immunol. 1985 Jun;134(6):4140–4145. [PubMed] [Google Scholar]
  22. Reinherz E. L., Penta A. C., Hussey R. E., Schlossman S. F. A rapid method for separating functionally intact human T lymphocytes with monoclonal antibodies. Clin Immunol Immunopathol. 1981 Nov;21(2):257–266. doi: 10.1016/0090-1229(81)90214-2. [DOI] [PubMed] [Google Scholar]
  23. Rubinstein L. J., Yeh M., Bona C. A. Idiotype-anti-idiotype network. II. Activation of silent clones by treatment at birth with idiotypes is associated with the expansion of idiotype-specific helper T cells. J Exp Med. 1982 Aug 1;156(2):506–521. doi: 10.1084/jem.156.2.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sacks D. L., Esser K. M., Sher A. Immunization of mice against African trypanosomiasis using anti-idiotypic antibodies. J Exp Med. 1982 Apr 1;155(4):1108–1119. doi: 10.1084/jem.155.4.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sharpe A. H., Gaulton G. N., McDade K. K., Fields B. N., Greene M. I. Syngeneic monoclonal antiidiotype can induce cellular immunity to reovirus. J Exp Med. 1984 Oct 1;160(4):1195–1205. doi: 10.1084/jem.160.4.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stein K. E., Söderström T. Neonatal administration of idiotype or antiidiotype primes for protection against Escherichia coli K13 infection in mice. J Exp Med. 1984 Oct 1;160(4):1001–1011. doi: 10.1084/jem.160.4.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tsoi M. S., Aprile J., Dobbs S., Goehle S., Storb R. Enrichment (and depletion) of human suppressor cells with monoclonal antibodies and immunoglobulin-coated plates. J Immunol Methods. 1982 Sep 30;53(3):293–305. doi: 10.1016/0022-1759(82)90176-4. [DOI] [PubMed] [Google Scholar]
  28. Tunón P., Johansson K. E. Yet another improved silver staining method for the detection of proteins in polyacrylamide gels. J Biochem Biophys Methods. 1984 May;9(2):171–179. doi: 10.1016/0165-022x(84)90008-3. [DOI] [PubMed] [Google Scholar]
  29. Unanue E. R. Antigen-presenting function of the macrophage. Annu Rev Immunol. 1984;2:395–428. doi: 10.1146/annurev.iy.02.040184.002143. [DOI] [PubMed] [Google Scholar]
  30. Van Wauwe J. P., Goossens J. G. The mitogenic activity of OKT3 and anti-Leu 4 monoclonal antibodies: a comparative study. Cell Immunol. 1983 Apr 1;77(1):23–29. doi: 10.1016/0008-8749(83)90003-5. [DOI] [PubMed] [Google Scholar]
  31. Wikler M., Demeur C., Dewasme G., Urbain J. Immunoregulatory role of maternal idiotypes. Ontogeny of immune networks. J Exp Med. 1980 Oct 1;152(4):1024–1035. doi: 10.1084/jem.152.4.1024. [DOI] [PMC free article] [PubMed] [Google Scholar]

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