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. 1986 Nov;78(5):1142–1149. doi: 10.1172/JCI112694

Identification of a major sialoprotein in the glycocalyx of human visceral glomerular epithelial cells.

D Kerjaschki, H Poczewski, G Dekan, R Horvat, E Balzar, N Kraft, R C Atkins
PMCID: PMC423796  PMID: 3533998

Abstract

Glomerular visceral epithelial cells are endowed with a sialic acid-rich surface coat (the "glomerular epithelial polyanion"), which in rat tissue contains the sialoprotein podocalyxin. We have identified a major membrane sialoprotein in human glomeruli that is similar to rat podocalyxin in its sialic acid-dependent binding of wheat germ agglutinin and in its localization on the surface of glomerular epithelial and endothelial cells, as shown by immunoelectron microscopy, using the monoclonal antibody PHM5. Differences in the sialoproteins of the two species are indicated by the discrepancy of their apparent molecular weights in sodium dodecyl sulfate gels, by the lack of cross reactivity of their specific antibodies, and by the lack of homology of their proteolytic peptide maps. It is therefore possible that the human glomerular sialoprotein and rat podocalyxin are evolutionarily distinct, but have similar functions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blau E. B., Haas J. E. Glomerular sialic acid and proteinuria in human renal disease. Lab Invest. 1973 Apr;28(4):477–481. [PubMed] [Google Scholar]
  2. Charest P. M., Roth J. Localization of sialic acid in kidney glomeruli: regionalization in the podocyte plasma membrane and loss in experimental nephrosis. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8508–8512. doi: 10.1073/pnas.82.24.8508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davis J. Q., Bennett V. Brain ankyrin. Purification of a 72,000 Mr spectrin-binding domain. J Biol Chem. 1984 Feb 10;259(3):1874–1881. [PubMed] [Google Scholar]
  4. Gahmberg C. G., Andersson L. C. Role of sialic acid in the mobility of membrane proteins containing O-linked oligosaccharides on polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Eur J Biochem. 1982 Mar 1;122(3):581–586. doi: 10.1111/j.1432-1033.1982.tb06478.x. [DOI] [PubMed] [Google Scholar]
  5. Green M. R., Pastewka J. V. Identification of sialic acid-rich glycoproteins on polyacrylamide gels. Anal Biochem. 1975 May 12;65(1-2):66–72. doi: 10.1016/0003-2697(75)90491-1. [DOI] [PubMed] [Google Scholar]
  6. Hancock W. W., Atkins R. C. Monoclonal antibodies to human glomerular cells: a marker for glomerular epithelial cells. Nephron. 1983;33(2):83–90. doi: 10.1159/000182918. [DOI] [PubMed] [Google Scholar]
  7. Hancock W. W., Kraft N., Clarke F., Atkins R. C. Production of monoclonal antibodies to fibronectin, type IV collagen and other antigens of the human glomerulus. Pathology. 1984 Apr;16(2):197–206. doi: 10.3109/00313028409059105. [DOI] [PubMed] [Google Scholar]
  8. Herrmann H., Wiche G. Specific in situ phosphorylation of plectin in detergent-resistant cytoskeletons from cultured Chinese hamster ovary cells. J Biol Chem. 1983 Dec 10;258(23):14610–14618. [PubMed] [Google Scholar]
  9. Holthöfer H., Virtanen I., Pettersson E., Törnroth T., Alfthan O., Linder E., Miettinen A. Lectins as fluorescence microscopic markers for saccharides in the human kidney. Lab Invest. 1981 Nov;45(5):391–399. [PubMed] [Google Scholar]
  10. Horvat R., Hovorka A., Dekan G., Poczewski H., Kerjaschki D. Endothelial cell membranes contain podocalyxin--the major sialoprotein of visceral glomerular epithelial cells. J Cell Biol. 1986 Feb;102(2):484–491. doi: 10.1083/jcb.102.2.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huang T. W., Langlois J. C. Podoendin. A new cell surface protein of the podocyte and endothelium. J Exp Med. 1985 Jul 1;162(1):245–267. doi: 10.1084/jem.162.1.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones D. B. Mucosubstances of the glomerulus. Lab Invest. 1969 Aug;21(2):119–125. [PubMed] [Google Scholar]
  13. Kerjaschki D., Farquhar M. G. Immunocytochemical localization of the Heymann nephritis antigen (GP330) in glomerular epithelial cells of normal Lewis rats. J Exp Med. 1983 Feb 1;157(2):667–686. doi: 10.1084/jem.157.2.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kerjaschki D., Sharkey D. J., Farquhar M. G. Identification and characterization of podocalyxin--the major sialoprotein of the renal glomerular epithelial cell. J Cell Biol. 1984 Apr;98(4):1591–1596. doi: 10.1083/jcb.98.4.1591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kerjaschki D., Vernillo A. T., Farquhar M. G. Reduced sialylation of podocalyxin--the major sialoprotein of the rat kidney glomerulus--in aminonucleoside nephrosis. Am J Pathol. 1985 Mar;118(3):343–349. [PMC free article] [PubMed] [Google Scholar]
  16. Michael A. F., Blau E., Vernier R. L. Glomerular polyanion. Alteration in aminonucleoside nephrosis. Lab Invest. 1970 Dec;23(6):649–657. [PubMed] [Google Scholar]
  17. Mohos S. C., Skoza L. Glomerular sialoprotein. Science. 1969 Jun 27;164(3887):1519–1521. doi: 10.1126/science.164.3887.1519. [DOI] [PubMed] [Google Scholar]
  18. Nolte A., Ohkuma M. Elektronenmikroskopisch-histochemische Untersuchungen zur Dartsellung saurer Mucopolysaccharide und sialinsäurehaltiger Glycoproteine in Nierenrinde und innerem Nierenmark der Ratte. Histochemie. 1969;17(2):170–180. doi: 10.1007/BF00277783. [DOI] [PubMed] [Google Scholar]
  19. Peters B. P., Ebisu S., Goldstein I. J., Flashner M. Interaction of wheat germ agglutinin with sialic acid. Biochemistry. 1979 Nov 27;18(24):5505–5511. doi: 10.1021/bi00591a038. [DOI] [PubMed] [Google Scholar]

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