Skip to main content
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1986 Dec;78(6):1443–1448. doi: 10.1172/JCI112734

Angiotensin II and vasopressin stimulate calcium-activated chloride conductance in rat mesangial cells.

T Okuda, N Yamashita, K Kurokawa
PMCID: PMC423888  PMID: 3782465

Abstract

In an attempt to clarify the mechanisms by which angiotensin II (AII) and arginine vasopressin (AVP) regulate mesangial cell function, we examined the membrane potential change of mesangial cells and found that cells contracted and membrane potential depolarized in response to AII and AVP. The depolarization was associated with decreased input resistance. Ca ionophore A23187 caused similar mesangial cell contraction and depolarization. The reversal potential (Vr) of the depolarization response to AII and AVP was -29 +/- 3 and -25 +/- 7 mV (mean +/- SD), respectively. Not only the Vr of the AII-induced depolarization but also Vr of the Ca ionophore-induced response was dependent upon the extracellular Cl- concentration. Further, AII and AVP caused cell contraction and membrane depolarization in Ca++-free medium containing 0.5 mM EGTA. These data suggest the presence of Ca++ -activated Cl- channels in the mesangial cells and that AII and AVP increase Cl- permeability via an elevation of [Ca++]i released from the intracellular organellae.

Full text

PDF
1443

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. M., Coffey A. K. Cytoplasmic contractile elements in glomerular cells. Fed Proc. 1983 Nov;42(14):3046–3052. [PubMed] [Google Scholar]
  2. Ardaillou N., Hagege J., Nivez M. P., Ardaillou R., Schlondorff D. Vasoconstrictor-evoked prostaglandin synthesis in cultured human mesangial cells. Am J Physiol. 1985 Feb;248(2 Pt 2):F240–F246. doi: 10.1152/ajprenal.1985.248.2.F240. [DOI] [PubMed] [Google Scholar]
  3. Ausiello D. A., Kreisberg J. I., Roy C., Karnovsky M. J. Contraction of cultured rat glomerular cells of apparent mesangial origin after stimulation with angiotensin II and arginine vasopressin. J Clin Invest. 1980 Mar;65(3):754–760. doi: 10.1172/JCI109723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barish M. E. A transient calcium-dependent chloride current in the immature Xenopus oocyte. J Physiol. 1983 Sep;342:309–325. doi: 10.1113/jphysiol.1983.sp014852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dworkin L. D., Ichikawa I., Brenner B. M. Hormonal modulation of glomerular function. Am J Physiol. 1983 Feb;244(2):F95–104. doi: 10.1152/ajprenal.1983.244.2.F95. [DOI] [PubMed] [Google Scholar]
  6. Ichikawa I., Brenner B. M. Glomerular actions of angiotensin II. Am J Med. 1984 May 31;76(5B):43–49. doi: 10.1016/0002-9343(84)90882-9. [DOI] [PubMed] [Google Scholar]
  7. Ichikawa I. Direct analysis of the effector mechanism of the tubuloglomerular feedback system. Am J Physiol. 1982 Nov;243(5):F447–F455. doi: 10.1152/ajprenal.1982.243.5.F447. [DOI] [PubMed] [Google Scholar]
  8. Kreisberg J. I. Contractile properties of the glomerular mesangium. Fed Proc. 1983 Nov;42(14):3053–3057. [PubMed] [Google Scholar]
  9. Kreisberg J. I., Karnovsky M. J. Glomerular cells in culture. Kidney Int. 1983 Mar;23(3):439–447. doi: 10.1038/ki.1983.40. [DOI] [PubMed] [Google Scholar]
  10. Lovett D. H., Ryan J. L., Sterzel R. B. Stimulation of rat mesangial cell proliferation by macrophage interleukin 1. J Immunol. 1983 Dec;131(6):2830–2836. [PubMed] [Google Scholar]
  11. MacCarthy E. P., Hsu A., Ooi Y. M., Ooi B. S. Evidence for a mouse mesangial cell-derived factor that stimulates lymphocyte proliferation. J Clin Invest. 1985 Aug;76(2):426–430. doi: 10.1172/JCI111989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maruyama Y., Petersen O. H. Voltage clamp study of stimulant-evoked currents in mouse pancreatic acinar cells. Pflugers Arch. 1983 Sep;399(1):54–62. doi: 10.1007/BF00652522. [DOI] [PubMed] [Google Scholar]
  13. Mayer M. L. A calcium-activated chloride current generates the after-depolarization of rat sensory neurones in culture. J Physiol. 1985 Jul;364:217–239. doi: 10.1113/jphysiol.1985.sp015740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Owen D. G., Segal M., Barker J. L. A Ca-dependent Cl- conductance in cultured mouse spinal neurones. Nature. 1984 Oct 11;311(5986):567–570. doi: 10.1038/311567a0. [DOI] [PubMed] [Google Scholar]
  15. Scharschmidt L. A., Dunn M. J. Prostaglandin synthesis by rat glomerular mesangial cells in culture. Effects of angiotensin II and arginine vasopressin. J Clin Invest. 1983 Jun;71(6):1756–1764. doi: 10.1172/JCI110931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schnermann J., Ploth D. W., Hermle M. Activation of tubulo-glomerular feedback by chloride transport. Pflugers Arch. 1976 Apr 6;362(3):229–240. doi: 10.1007/BF00581175. [DOI] [PubMed] [Google Scholar]
  17. Taugner R., Schiller A., Kaissling B., Kriz W. Gap junctional coupling between the JGA and the glomerular tuft. Cell Tissue Res. 1978 Jan 17;186(2):279–285. doi: 10.1007/BF00225537. [DOI] [PubMed] [Google Scholar]
  18. Venkatachalam M. A., Kreisberg J. I. Agonist-induced isotonic contraction of cultured mesangial cells after multiple passage. Am J Physiol. 1985 Jul;249(1 Pt 1):C48–C55. doi: 10.1152/ajpcell.1985.249.1.C48. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES