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. 2014 Nov 21;36(6):9726. doi: 10.1007/s11357-014-9726-0

Plantaris muscle weakness in old mice: relative contributions of changes in specific force, muscle mass, myofiber cross-sectional area, and number

Sam B Ballak 1,2, Hans Degens 1, Tinelies Busé-Pot 2, Arnold de Haan 1,2, Richard T Jaspers 2,✉
PMCID: PMC4239237  PMID: 25414077

Abstract

The age-related decline in muscle function contributes to the movement limitations in daily life in old age. The age-related loss in muscle force is attributable to loss of myofibers, myofiber atrophy, and a reduction in specific force. The contribution of each of these determinants to muscle weakness in old age is, however, largely unknown. The objective of this study is to determine whether a loss in myofiber number, myofiber atrophy, and a reduction in specific muscle force contribute to the age-related loss of muscle force in 25-month-old mouse. Maximal isometric force of in situ m. plantaris of C57BL/6J male adult (9 months) and old (25 months) mice was determined and related to myofiber number, myofiber size, intramuscular connective tissue content, and proportion of denervated myofibers. Isometric maximal plantaris muscle force was 13 % lower in old than adult mice (0.97 ± 0.05 N vs. 0.84 ± 0.03 N; P < 0.05). M. plantaris mass of old mice was not significantly smaller than that of adult mice. There was also no significant myofiber atrophy or myofiber loss. Specific muscle force of old mice was 25 % lower than that of adult mice (0.55 ± 0.05 vs. 0.41 ± 0.03 N·mm−2, P < 0.01). In addition, with age, the proportion of type IIB myofibers decreased (43.6 vs. 38.4 %, respectively), while the connective tissue content increased (11.6 vs. 16.4 %, respectively). The age-related reduction in maximal isometric plantaris muscle force in 25-month-old male C57BL/6J mice is mainly attributable to a reduction in specific force, which is for 5 % explicable by an age-related increase in connective tissue, rather than myofiber atrophy and myofiber loss.

Keywords: Sarcopenia, Muscle aging, Myofiber atrophy, Myofiber loss, Specific force, C57BL/6 mice

Introduction

Aging is associated with a progressive decline in muscle power generating capacity (Runge et al. 2004) that contributes to an increased incidence of falls, a decreased independence and reduced quality of life (Kamel 2003; Roubenoff and Hughes 2000). Improvement of muscle function in the older person may alleviate many of these problems. To develop strategies to preserve, slow, or even reverse muscle weakness in advanced age, a profound understanding of the etiology of age-related muscle weakness is needed.

An important determinant of muscle power is the force generating capacity of the muscle. Muscle force generating capacity in humans can decrease as much as 60 % between the age of 30 and 80 years (Faulkner et al. 2007). Also in rodents, a 25–65 % age-related deterioration in the muscle force generating capacity has been reported (Ballak et al. 2014; Brooks and Faulkner 1988; Carter et al. 2010; Degens and Alway 2003), which is not only due to a loss of muscle mass but also a reduction in the force-generating capacity per muscle cross-sectional area, specific force (Degens et al. 2009). It could be that during the initial stages of sarcopenia a reduction in specific force precedes the loss in muscle mass.

Concomitant with the loss of muscle force generating capacity there is a loss of muscle mass. In the human m. vastus lateralis, this amounted to approximately 30 % between 40 and 80 years (Janssen et al. 2000; Klitgaard et al. 1990; Maden-Wilkinson et al. 2014; Overend et al. 1992; Rice et al. 1989; Young et al. 1984; Young et al. 1985). In rodents, at ages representative of these ages in humans, a similar age-related reduction in muscle mass is observed (Ballak et al. 2014). Typically, the age-related decrease in muscle mass is due to both a reduction in myofiber number (Lexell et al. 1988; Lushaj et al. 2008; McKiernan et al. 2004; Sato et al. 1984; Zerba et al. 1990) and (preferential type II) myofiber atrophy (Brown and Hasser 1996; Carter et al. 2010; Klitgaard et al. 1990; Larsson 1978; Tomonaga 1977; Verdijk et al. 2007). However, the loss in maximal muscle force with age is more than proportional to the decrease in muscle mass (Degens et al. 2009). This implies age-related intrinsic changes of the muscle affecting the quality of the remaining muscle tissue (Frontera et al. 2008). In fact, several studies in both humans (Frontera et al. 2008; Klein et al. 2001; Morse et al. 2005) and rodents (Degens and Alway 2003; Degens et al. 1995; Kadhiresan et al. 1996; Rice et al. 2005) have reported a reduced force per unit cross-sectional area (referred to as specific force) during aging. An increased connective tissue content (Brooks and Faulkner 1994; Lushaj et al. 2008; Overend et al. 1992), inclusion of denervated, and thus non-force-producing myofibers (Urbanchek et al. 2001), and a selective atrophy of type II myofibers that reportedly have a higher specific force than type I fibers (Larsson et al. 1997a; Yu et al. 2007), may also contribute to the age-related reduction of specific muscle force.

It thus appears that the three prime factors that contribute to the age-related muscle weakness are a reduction in myofiber number, myofiber atrophy, and a reduction in specific force. Yet, these determinants of the age-related muscle dysfunction are hitherto not evaluated collectively, especially in early stages of sarcopenia. Since the contribution of each of these determinants to the age-related changes in muscle force generating capacity cannot be investigated in humans, we used m. plantaris in adult (9 months) and old (25 months) mice. We hypothesized that skeletal muscle weakness in old age is in order of importance due to (a) a loss in muscle quantity, (b) a reduction in muscle quality.

To investigate this, we determined the maximal isometric force of mouse m. plantaris in situ. To determine the relative contribution of the three abovementioned factors, tetanic force was related to m. plantaris mass, myofiber CSA, myofiber number, and specific force. Histological analysis was also performed to determine connective tissue content and proportion of denervated, and hence non-force producing, myofibers.

Methods

Animals

Experiments were performed on eleven 9-month-old (adult) and ten 25-month-old (old) male C57BL/6J mice (Janvier, France). Mice were housed individually and kept under specific-pathogen-free conditions at 20–22 °C at a 12 h light/dark cycle. Animals were given free access to water and chow (Ssniff® S8189-S095). For the experiments described below, we analyzed histological and contractile force characteristics of the right leg. At the age of 7.5 (adult) or 23.5 (old) months, in the left leg of the mice the m. gastrocnemius and m. soleus were denervated to overload the m. plantaris muscle for 6 weeks. The effects of overload are beyond the scope of the present study.

All experiments were approved by the local animal use and care committee of the VU University Amsterdam and conformed to the guide of the Dutch Research Council for care and use of laboratory animals.

Surgery and preparation

Fifteen minutes prior to the start of the experiment, mice received a subcutaneous injection of 0.06 mL 1 % Temgesic (Reckitt Benckiser, UK) as an analgesic and were anesthetized with 4 % isoflurane, 0.1 L min−1 O2 and 0.2 L min−1 air. Subsequently, the level of anesthesia was maintained with 1.5–2.5 % isoflurane. A humidifier moistened the inhaled air to prevent dehydration due to respiration. The mice were placed on a heated pad to maintain body temperature at ∼36.5 °C.

The experiments were performed as explained previously (Degens and Alway 2003). The m. plantaris was dissected free from surrounding tissue while maintaining its innervation and blood supply. The sciatic nerve was severed and the proximal end was placed over an electrode for stimulation of the muscle. The distal tendon of the m. plantaris was dissected free and tightened with a Kevlar thread via a small steel link to a force transducer, which was mounted on the lever arm of an isovelocity measuring system. The femur was fixed by a clamp on the condyle of the femur. During the experiment, the muscle and its surrounding were kept moist at physiological temperature (34–36 °C) with a water-saturated airflow.

Experimental setup and force measurements

Figure 1 shows a diagram of the experimental setup for in situ muscle force measurements which is a modification of that described (de Haan et al. 1989). Contractions were induced by supramaximal electrical stimulation of the sciatic nerve at a constant current (2 mA; 200 μs pulse width). Optimal muscle length (ℓo) was defined as the muscle length at which maximal tetanic isometric force was generated. To determine ℓo, first the length at which the muscle produced maximal twitch force was assessed. To fine adjust ℓo tetani (150 Hz, 150 ms) were applied once every 2 min. Two minutes after the last tetanus the muscle was stimulated by applying a 400-Hz, 80-ms pulse train to determine the maximal rate of force development (MRTD). Finally, the muscle was stimulated by applying a 150-Hz, 150-ms pulse trains once every second for 5 min to deplete the glycogen in fibers expressing type IIB myosin. Force and length signals (1–10 kHz) were stored on disk. At the end of the experiment, the m. plantaris was excised and weighed and the mouse was killed by cervical dislocation.

Fig. 1.

Fig. 1

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Experimental setup as used during all measurements. This is a modification of the setup used by de Haan et al. (1989). The anesthetized mouse was placed on a heated pad, while the m. plantaris was attached to the force transducer, which allowed to measure muscle force in situ

Analyses

Tissue preparation and cutting of histological sections

The m. plantaris was embedded at ℓo in a gelatin tyrode (NaCl, 128.3 mM; KCl, 4.7 mM; MgCl2, 1.05 mM; NaH2PO4H2O, 0.42 mM; NaHCO3, 20.2 mM; EGTA, 15.0 mM; Gelatine 15 % (w/v), pH 7.2) solution and frozen in liquid nitrogen. Subsequently, m. soleus and m. gastrocnemius medialis were excised and also frozen in liquid nitrogen. All chemicals were obtained from Sigma Aldrich (The Netherlands) unless stated otherwise.

Within a month after the contraction protocol, serial cross-sections (10 μm) were cut from the midbelly of the m. plantaris using a cryostat at −20 °C. The in-gelatin tyrode-embedded sections were mounted with Tissue-Tek (Jung, Leica Microsystems, Germany) onto a specimen holder, avoiding direct contact between Tissue-Tek and the part of the muscle that would be sectioned to prevent freeze-thaw artifacts. Sections were mounted on glass slides (Menzel-Gläser, superfrost® plus, Germany), air-dried, and stored at −80 °C until further use.

Myosin heavy chain composition

Serial sections of the m. plantaris were immunohistochemically stained against type I, IIA, IIX, and IIB MHC using monoclonal antibodies BAD5 (1 μg/mL), SC-71 (1 μg/mL), 6H1 (10 μg/mL), and BF-F3 (1–10 μg/mL) (Developmental Studies Hybridoma Bank, USA), respectively. In short, sections were fixed with acetone for 10 min at 4 °C and washed in phosphate-buffered saline with 0.05 % tween (PBST) three times for 5 min. After blocking with 10 % normal swine serum for 30 min, the sections were incubated with the primary antibody for 90 min. Subsequently, sections were washed in PBST three times for 3 min and incubated in the dark with secondary antibody (Alexa 488 anti-mouse, 1:500, Life Technologies, The Netherlands) for 30 min. After washing with PBST, incubating with wheat germ agglutinin (WGA, 1:50, Life Technologies, The Netherlands) for 20 min, washing with PBST, and afterwards washing once more with PBS all in the dark, the sections were enclosed with Vectashield®-hardset mounting medium with DAPI (1.5 μg/mL; Vector Laboratories, USA). The public domain software ImageJ 1.45s (Rasband, W.S., ImageJ, US National Institutes of Health, Bethesda, MD, USA) was used to assess myofiber number, type, and myofiber cross-sectional area.

Determination of denervated myofibers by PAS-staining

The glycogen content of individual myofibers was determined in 10-μm-thick sections using the periodic acid–Schiff (PAS) staining reaction (van der Laarse et al. 1992). The sections were air-dried and fixed for 5 min in 4 % formaldehyde in 20 mL, 0.2 M imidazole, and 180 mL acetone. Subsequently, sections were incubated in 44 mM periodic acid solution for 30 min at room temperature. After incubation, sections were briefly washed in 0.1 M HCl and stained with Schiff’s reagents for 25 min at room temperature.

Intramuscular connective tissue content

Intramuscular connective tissue was determined using Sirius Red. The sections were air-dried and fixed for 30 min in Bouin solution. Next, sections were washed for 10 min, before 30 min incubation in Sirius Red saturated with picric acid. Sections were dehydrated by rapidly dipping in absolute ethanol. For a better image quality, sections were cleared with xylene and mounted with DPX (Sigma Aldrich, UK). A Matlab-script (version R2012a) was used to quantify the connective tissue content per image. The RBG threshold was set at R > 140, B < 110, G < 110, to create a binary image allowing to filter all red pixels. The same threshold was applied to all images that were stained at the same time.

Statistics

To determine significant differences between age groups, Student’s t tests were used. To test for differences in fiber CSA, a two-way ANOVA was used with factors age and fiber type. Effects were considered significant at P < 0.05. Data are expressed as mean ± SEM. All calculations were performed using IBM SPSS version 20.

Results

Mice characteristics

Table 1 shows that body mass, muscle optimum length (ℓo), myofiber number, and muscle mass/body mass ratio did not differ significantly between adult and old mice. The masses of the plantaris, gastrocnemius medialis, and soleus muscles (PGMS) combined were -8 % less in old than adult animals (P = 0.03; Table 1). Mass of m. plantaris alone was, however, not significantly reduced in the old compared to the adult group (P > 0.05; Table 1).

Table 1.

Mice characteristics

Adult mice (n = 11) Old mice (n = 10)
Body mass (g) 36.1 ± 3.0 35.6 ± 0.9
PGMS muscle mass (mg) 102.6 ± 2.5 94.7 ± 2.3a
Plantaris muscle mass (mg) 23.6 ± 0.6 22.4 ± 0.7
Muscle ACSA (mm2) 1.83 ± 0.12 2.08 ± 0.10
Pooled myofiber CSA (μm2) 1498 ± 65 1546 ± 110
ℓ o(mm) 12.7 ± 0.37 12.88 ± 0.38
Myofiber number (#) 1027 ± 58 1104 ± 55
Ratio muscle/body mass (mg·g−1) 0.67 ± 0.04 0.63 ± 0.03
Denervated myofibers (%)
  %total 1.15 ± 0.37 1.33 ± 0.38
  %IIB 2.33 ± 0.68 3.48 ± 1.09
Specific force (N·mm−2) 0.55 ± 0.05 0.41 ± 0.03a
Specific force (N·g−1) 40.94 ± 1.4 37.58 ± 1.4
MRTD (mN·ms−1) 32.3 ± 2.1 29.1 ± 2.1
MRTD/Fmax (mN·ms−1·N−1) 36.4 ± 2.2 37.9 ± 1.5
RT50 (ms) 28.1 ± 0.9 32.3 ± 2.8 a
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Values are mean ± SEM

PGMS plantaris, gastrocnemius medialis, and soleus; ℓ o plantaris muscle length at which tetanic force was maximal; MRTD maximal rate of force development; RT50 half relaxation time

aSignificantly different from adult mice at P < 0.05

Age-related changes in morphology and in situ force characteristics of m. plantaris

The ACSA of the m. plantaris muscle did not differ significantly between adult and old mice (Table 1). Maximal isometric force of m. plantaris of old mice was 13 % lower compared to that of adults (Fig. 2a, c; P < 0.05). Maximal tetanic force divided by muscles ACSA (specific tetanic force) of old muscles was 25 % lower than that of adult muscle (P < 0.01; Fig. 2d). Five percent of this lower specific force was explicable by the higher relative content of connective tissue in the old (16.4 vs. 11.6 % in adult) muscles (specific muscle force corrected for collagen content: adult 0.63 ± 0.06 N·mm−2; vs old 0.50 ± 0.03 N·mm−2; P =0.087). However, specific tetanic force expressed as maximal tetanic force per muscle mass did not differ significantly between adult and old muscles (Table 1).

Fig. 2.

Fig. 2

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Maximal isometric tetanus force (N) during a 150 Hz isometric tetanus plotted against time (ms) for adult and old m. plantaris of mice (a). Force (N) during a 400 Hz isometric tetanus plotted against time for adult and old m. plantaris of mice (b). Maximal force (mN) in adult mice was higher than that in old mice (*P < 0.05) (c). Specific force (N·mm−2) was less in old than adult m. plantaris (*P < 0.05). Values are mean ± SEM

Figure 3 shows examples of m. plantaris cross-sections stained specifically for type IIB myosin heavy chain. The percentage of type IIB myofibers decreased with age (43.6 vs. 38.4 % in adult and old, respectively; P < 0.05; Fig. 3c). The CSAs of type IIA, IIX, and IIB myofibers did not differ significantly between adult and old mice (Fig. 3d). Likewise, pooled CSA did not change significantly with age (Table 1).

Fig. 3.

Fig. 3

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Examples of immunofluorescent staining for type IIB myosin in an adult (a) and old (b) m. plantaris. Bar represents 500 μm. Type IIB myofiber proportion (%) in adult and old mice was significantly different (P < 0.05). Type I, IIA, IIAX, IIX, and IIXB proportions did not change significantly (c). Myofiber CSA (μm2) did not significantly decline for any fiber type (P > 0.05) (d). Note that type I myofibers were not included in this analysis, because of the low presence of type I myofibers within the m. plantaris of C57Bl/6 mice. Values are mean ± SEM

Figure 4a, b show examples of Sirius Red-stained muscle cross-sections. The percentage of collagen in the m. plantaris was higher in the old group compared to that in the adult group (16.4 vs. 11.6 %, respectively; P = 0.03; Fig. 4c). Figure 5 shows examples of serial muscle sections stained for type IIB myosin and PAS to quantify the number of denervated myofibers that express IIB myosin. The percentage of all fibers and the percentage of IIB myosin expressing myofibers that were denervated did not differ significantly between adult and old mice (Fig. 5e).

Fig. 4.

Fig. 4

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Collagen staining of m. plantaris of adult (a) and old mice (b). Bar represents 100 μm. Threshold analysis (c) revealed a significant age-related increase (*P < 0.05). Values are mean ± SEM

Fig. 5.

Fig. 5

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Serial sections stained for type IIB myosin and PAS of adult (a and b) and old (c and d) muscles. Bar represents 100 μm. Corresponding denervated myofibers are specified with an asterisk. Analysis revealed no significant difference between adult and old denervated myofiber count expressed as percentage of all IIB myofibers (e). Values are mean ± SEM

Maximal rate of force development (MRTD) did not significantly differ between adult and old plantaris muscles (Fig. 2b; Table 1). Also, normalized MRTD (MRTD/Fmax) did not decrease with age. However, half relaxation time of the 400 Hz contraction was significantly longer in the old than in adult muscles (P < 0.01) (Table 1), indicating that the old muscles were slower.

Discussion

The main finding of the present study is that the age-related loss in maximal force of plantaris muscle in 25-month-old male C57BL/6 mice is mainly attributable to a reduction in specific force. In contrast to our hypothesis, plantaris muscles of old mice did not show a reduction in total and functional myofiber number, nor myofiber CSA.

Age-related decline in force generating capacity is explained by loss of muscle mass and reductions in specific force

Here, we observed a 13 % lower force generating capacity in 25- compared to male 9-month-old mice. Our aim was to determine to what extent reductions in muscle mass (quantity) and specific muscle force (quality) contribute to this age-related decline in muscle force generating capacity.

Other studies in male mice have reported that the ∼25 % age-related decline in muscle force in both slow and fast hindlimb muscles is explained for 55 % by the age-related loss of muscle mass, and for ∼45 % by a reduction in specific force in 26–28-month-old mice (Brooks and Faulkner 1988; 1991; Lynch et al. 2001; Zerba et al. 1990). We did not observe a decrease in m. plantaris mass which is different from what has been reported in other studies in which a 15–26 % lower muscle mass was reported in 26–28-month-old mice (Brooks and Faulkner 1988; Jackson et al. 2011; Lynch et al. 2001; McArdle et al. 2004). The discrepancy between the effects of aging on muscle mass and maximal force shown in our study and those previously reported is unlikely related to the muscles used, as other studies observed the effects of aging in both fast and slow muscles. It is more likely that the use of older animals (26–28 months) in other studies than in our study (25 months) contributes to the discrepancy, as larger effects of aging on maximal muscle force are likely to be observed in the older the animal (Ballak et al. 2014; Brooks and Faulkner 1988; Lynch et al. 2001; McArdle et al. 2004). Indeed, Chan and Head (2010) observed that even in 20–22-month-old mice, specific force was already reduced without significant muscle atrophy. Combination of the data of these studies with slightly older and younger mice than the ones used in the present study suggests that a reduction in muscle quality precedes a reduction in muscle mass. If so, it is likely that the contribution of a loss of muscle mass to the age-related muscle weakness increases with increasing age.

Effects of aging on atrophy and loss in myofiber number

An age-related reduction in muscle mass can be the result of both a loss of myofibers and myofiber atrophy (Lexell et al. 1988; Lushaj et al. 2008). Here, no myofiber atrophy nor myofiber loss was observed. Our observations are in line with other reports in which age-related decline in myofiber CSA could not be observed in m. extensor digitorum longus and m. soleus of 24- compared to 5-month-old mice (Sheard and Anderson 2012; Zerba et al. 1990). It is possible that the age-related myofiber atrophy and loss occur only at a later age as a small decrease in myofiber number of 10–15 % has been observed in m. extensor digitorum longus and m. soleus of 24–27-month-old C57Bl/6 mice (Sheard and Anderson 2012; Zerba et al. 1990). Also, human myofiber loss has not unequivocally been reported. Recently, in m. vastus lateralis of healthy 70-year-old subjects, a decrease in muscle mass was solely explained by a decrease in type II fiber CSA, and not by a loss of myofibers (Nilwik et al. 2013). Overall, it appears that in 25-month-old male C57BL/6J mice myofiber number size are well maintained. Therefore, if these factors are involved in aging-related reduction in maximal muscle force, the impact on the decline in maximal isometric muscle force will be minor and may only become significant at later stages of the aging process.

Effects of aging on fibrosis, denervation, and myofiber type changes

The specific force (force per anatomical cross-sectional area) of 25-month-old m. plantaris was 25 % lower compared to that of 9-month-old mice. The magnitude of the decrease in specific force was similar to that reported by others (Brooks and Faulkner 1988; Lynch et al. 2001; McArdle et al. 2004). Therefore, the age-related decrease in specific force appears to be an important factor contributing to an age-related decline in muscle force.

Theoretically, the loss of specific muscle force may be the result of an increase in connective tissue content, a larger proportion of denervated myofibers, a decrease in the proportion of fast type IIB myofibers, and/or a reduced force generating capacity of individual myofibers (reduced specific force). The fraction of intramuscular connective tissue within the m. plantaris was higher in muscles from old than from adult animals. Others have also observed an increase in connective tissue content with age (Brooks and Faulkner 1994; Lushaj et al. 2008; Overend et al. 1992). When we corrected the specific force for the connective tissue content, the difference between adult and old muscles was not significant anymore. Thus, part of the reduction in specific force in muscles from old mice is attributable to an increased content of connective tissue.

Another factor that could potentially reduce specific muscle force is an age-related increase in the number of denervated myofibers as a result of motor neuron death (Hashizume et al. 1988; Ishihara et al. 1987; Larsson and Ansved 1995). Using glycogen depletion, we were able to assess the proportion of non-recruited, presumable denervated fibers. However, no significant difference in the proportion of non-recruited myofibers was observed between the age groups, and therefore the lower specific force in the older animals is not attributable to loss of functional myofibers.

The proportion of myofibers expressing type IIB myosin decreased with age. The longer half relaxation time of the 400 Hz tetanus in aged than in adult muscle fits this observed reduction in the proportion of fibers expressing type IIB myosin. It is debatable, however, how much this actually contributes to the lower specific force. It is controversial whether the specific force of type IIB fibers is higher than that of other fiber types (Bottinelli et al. 1996; Widrick et al. 1996) or not (Degens and Larsson 2007; Larsson and Moss 1993). Even if specific force of type IIB fibers was 1.41 times as high of that of other fibers (Larsson and Moss 1993; Bottinelli et al. 1996 IIB vs. I) the decrease from 44 % IIB fibers in adult to 38 % in old muscle would only cause a 2 % reduction in specific force. This is even an overestimation as the plantaris muscle of the mouse barely contains type I fibers and the differences in specific force between fast fibers, if existent, are much smaller than those reported in (Bottinelli et al. 1996) between type I and type IIB fibers. It is thus unlikely that the fiber type shift contributes to the observed age-related decline in specific force.

Finally, the observed age-related decrease in specific muscle force may be due to a loss of specific myofiber force with age (D’Antona et al. 2003; Gonzalez et al. 2000; Larsson et al. 1997a, b; Lowe et al. 2001; Thompson and Brown 1999). This reduced skeletal muscle force is possibly related to a decreased number of cross-bridges (D’Antona et al. 2003), oxidative modifications (Degens and Larsson 2007; Gilliver et al. 2010; Lowe et al. 2001) and/or an impaired excitation-contraction uncoupling (Delbono 2002; Gonzalez et al. 2000).

Muscle aging in 25-month-old mice mimics the initial stages of sarcopenia in humans

The decreases in maximal muscle force observed in this and other mice studies are rather small, compared to the effects reported at a similar relative mean life expectancy in humans (see, for review, Ballak et al. 2014). Nevertheless, the 13 % decrease in muscle strength between 9- and 25 months of age in our C57BL/6J mice is comparable to that between the age of 25–30-year-old and 60–70-year-old humans (Ballak et al. 2014).

Implications

Future studies could investigate the time course of the relative contribution of decrements in specific force and muscle mass to the loss of muscle strength. To do so, both somewhat younger and also older C57BL/6J mice (e.g., 26–30 months) should be studied, while making sure that the impact of disease is minimized. Furthermore, nutritional intake may be changed (e.g., lower protein intake) to observe whether a poor diet causes not only a lower specific muscle force and a larger loss of muscle mass at a given age, but also an accelerated rate of sarcopenia.

To counteract sarcopenia, it is essential to know the mechanisms and causes of muscle aging, especially in the initial stages of sarcopenia. The present data suggest that interventions should, at least during the early stages of sarcopenia, aim primarily to stop or reverse the age-related decline in specific muscle force. This could be achieved by resistance training that has been shown to increase specific force (Erskine et al. 2011). The impact of training may decrease with increasing age (Degens 2012; Slivka et al. 2008) and it thus appears essential to start interventions before sarcopenia takes its toll.

Conclusion

In conclusion, the age-related reductions in maximal isometric muscle force in male C57BL/6J mice were mainly attributable to a reduced specific force. Part of the reduction in specific force was due to an age-related increase in connective tissue content, but not to an increase in the percentage of denervated IIB myofibers. It is conceivable that the age-related reduction in specific force is the result of loss of specific force of individual myofibers. We did not observe an age-related decline of myofiber number and myofiber CSA. Thus, our model seems to mimic force reductions similar to those reported in the early stages of sarcopenia in humans, and it is suggested that the decline in muscle quality precedes the decline in muscle quantity with age. Therefore, interventions to constrain early sarcopenia should aim particularly to improve specific muscle force.

Acknowledgments

This research was funded by the European Commission through MOVE-AGE, an Erasmus Mundus Joint Doctorate program (2011-2015). The authors would like to thank Guus Baan for excellent technical assistance. Furthermore, we would like to acknowledge Frank van’t Hoff and Joshua Dunnink for their support.

Disclosure statement

The authors declare no conflicts of interest, financial or otherwise. The sponsor was in no way involved in the design or execution of this study.

Footnotes

• Lower specific muscle force rather than muscle atrophy explains reduced maximal isometric force in 25- compared to 9-month-old mice.

• Part of the reduction in specific muscle force was due to an age-related increase in connective tissue content.

• No age-related decline in myofiber number nor CSA was observed.

• If early effects of aging on human muscle are similar as those on mouse plantaris muscle, interventions to constrain early sarcopenia should aim at improving specific muscle force.

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