Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1986 Dec;78(6):1612–1620. doi: 10.1172/JCI112754

Regulation by adrenal corticosteroids of sodium and potassium transport in loop of Henle and distal tubule of rat kidney.

B A Stanton
PMCID: PMC423933  PMID: 3023448

Abstract

Studies were conducted to examine the effects of adrenalectomy (ADX) and selective, physiological adrenal corticosteroid replacement on sodium and potassium transport by the superficial loop of Henle and distal tubule of rat kidney in vivo. In the loop of Henle, ADX inhibited sodium reabsorption by 33%. Whereas dexamethasone had no effect on reabsorption, aldosterone increased sodium transport to control levels. Thus, physiological levels of mineralocorticoids, but not glucocorticoids, control a fraction of sodium reabsorption in the loop of Henle. ADX also inhibited potassium reabsorption in the loop of Henle. Both dexamethasone and aldosterone reversed the inhibition, although only aldosterone increased reabsorption to control levels. In the distal tubule, ADX reduced sodium reabsorption by 44%. Both aldosterone and dexamethasone stimulated reabsorption: however, only aldosterone increased transport to control. Potassium secretion by the distal tubule was also reduced 34% by ADX. Aldosterone, but not dexamethasone, stimulated secretion. Thus, physiological levels of aldosterone regulate a fraction of sodium reabsorption and potassium secretion in the distal tubule.

Full text

PDF
1612

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERLINER R. W., DAVIDSON D. G. Production of hypertonic urine in the absence of pituitary antidiuretic hormone. J Clin Invest. 1957 Oct;36(10):1416–1427. doi: 10.1172/JCI103541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baylis C., Brenner B. M. Mechanism of the glucocorticoid-induced increase in glomerular filtration rate. Am J Physiol. 1978 Feb;234(2):F166–F170. doi: 10.1152/ajprenal.1978.234.2.F166. [DOI] [PubMed] [Google Scholar]
  3. Cooke C. R., Steenburg R. W. Effects of aldosterone and cortisol on the renal concentrating mechanism. J Lab Clin Med. 1973 Nov;82(5):784–792. [PubMed] [Google Scholar]
  4. Cortney M. A. Renal tubular transfer of water and electrolytes in adrenalectomized rats. Am J Physiol. 1969 Mar;216(3):589–598. doi: 10.1152/ajplegacy.1969.216.3.589. [DOI] [PubMed] [Google Scholar]
  5. Edelman I. S. Receptors and effectors in hormone action on the kidney. Am J Physiol. 1981 Oct;241(4):F333–F339. doi: 10.1152/ajprenal.1981.241.4.F333. [DOI] [PubMed] [Google Scholar]
  6. El Mernissi G., Doucet A. Short-term effect of aldosterone on renal sodium transport and tubular Na-K-ATPase in the rat. Pflugers Arch. 1983 Oct;399(2):139–146. doi: 10.1007/BF00663910. [DOI] [PubMed] [Google Scholar]
  7. Farman N., Bonvalet J. P. Aldosterone binding in isolated tubules. III. Autoradiography along the rat nephron. Am J Physiol. 1983 Nov;245(5 Pt 1):F606–F614. doi: 10.1152/ajprenal.1983.245.5.F606. [DOI] [PubMed] [Google Scholar]
  8. Field M. J., Stanton B. A., Giebisch G. H. Differential acute effects of aldosterone, dexamethasone, and hyperkalemia on distal tubular potassium secretion in the rat kidney. J Clin Invest. 1984 Nov;74(5):1792–1802. doi: 10.1172/JCI111598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Garg L. C., Narang N., Wingo C. S. Glucocorticoid effects on Na-K-ATPase in rabbit nephron segments. Am J Physiol. 1985 Apr;248(4 Pt 2):F487–F491. doi: 10.1152/ajprenal.1985.248.4.F487. [DOI] [PubMed] [Google Scholar]
  10. Green H. H., Harrington A. R., Valtin H. On the role of antidiuretic hormone in the inhibition of acute water diuresis in adrenal insufficiency and the effects of gluco- and mineralocorticoids in reversing the inhibition. J Clin Invest. 1970 Sep;49(9):1724–1736. doi: 10.1172/JCI106390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gross J. B., Kokko J. P. Effects of aldosterone and potassium-sparing diuretics on electrical potential differences across the distal nephron. J Clin Invest. 1977 Jan;59(1):82–89. doi: 10.1172/JCI108625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gutsche H. U., Müller-Suur R., Samwer K. F., Beer G., Hierholzer K. Tubuloglomerular feedback control in kidneys of adrenalectomized rats. Pflugers Arch. 1980 Jul;386(1):11–19. doi: 10.1007/BF00584181. [DOI] [PubMed] [Google Scholar]
  13. Hebert S. C., Andreoli T. E. Control of NaCl transport in the thick ascending limb. Am J Physiol. 1984 Jun;246(6 Pt 2):F745–F756. doi: 10.1152/ajprenal.1984.246.6.F745. [DOI] [PubMed] [Google Scholar]
  14. Higashihara E., Kokko J. P. Effects of aldosterone on potassium recycling in the kidney of adrenalectomized rats. Am J Physiol. 1985 Feb;248(2 Pt 2):F219–F227. doi: 10.1152/ajprenal.1985.248.2.F219. [DOI] [PubMed] [Google Scholar]
  15. Horster M., Schmid H., Schmidt U. Aldosterone in vitro restores nephron Na-K-ATPase of distal segments from adrenalectomized rabbits. Pflugers Arch. 1980 Apr;384(3):203–206. doi: 10.1007/BF00584554. [DOI] [PubMed] [Google Scholar]
  16. Kone B. C., Madsen K. M., Tisher C. C. Ultrastructure of the thick ascending limb of Henle in the rat kidney. Am J Anat. 1984 Oct;171(2):217–226. doi: 10.1002/aja.1001710207. [DOI] [PubMed] [Google Scholar]
  17. Landwehr D. M., Schnermann J., Klose R. M., Giebisch G. Effect of reduction in filtration rate on renal tubular sodium and water reabsorption. Am J Physiol. 1968 Sep;215(3):687–695. doi: 10.1152/ajplegacy.1968.215.3.687. [DOI] [PubMed] [Google Scholar]
  18. Levine D. Z., McLeod R. A., Byers M. K. Flow correlation of loop of Henle potassium influx. Can J Physiol Pharmacol. 1978 Jun;56(3):533–535. doi: 10.1139/y78-084. [DOI] [PubMed] [Google Scholar]
  19. Marver D. Evidence of corticosteroid action along the nephron. Am J Physiol. 1984 Feb;246(2 Pt 2):F111–F123. doi: 10.1152/ajprenal.1984.246.2.F111. [DOI] [PubMed] [Google Scholar]
  20. Morgan T., Berliner R. W. A study by continuous microperfusion of water and electrolyte movements in the loop of Henle and distal tubule of the rat. Nephron. 1969;6(3):388–405. doi: 10.1159/000179741. [DOI] [PubMed] [Google Scholar]
  21. Mujais S. K., Chekal M. A., Jones W. J., Hayslett J. P., Katz A. I. Modulation of renal sodium-potassium-adenosine triphosphatase by aldosterone. Effect of high physiologic levels on enzyme activity in isolated rat and rabbit tubules. J Clin Invest. 1985 Jul;76(1):170–176. doi: 10.1172/JCI111942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Murayama Y., Suzuki A., Tadokoro M., Sakai F. Microperfusion of Henle's loop in the kidney of the adrenalectomized rat. Jpn J Pharmacol. 1968 Dec;18(4):518–519. doi: 10.1254/jjp.18.518. [DOI] [PubMed] [Google Scholar]
  23. Peart W. S., Pessina A. C. The mechanism of acute renal ischaemia caused by adrenalectomy in the rat. J Physiol. 1975 Aug;250(1):23–37. doi: 10.1113/jphysiol.1975.sp011041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rastegar A., Biemesderfer D., Kashgarian M., Hayslett J. P. Changes in membrane surfaces of collecting duct cells in potassium adaptation. Kidney Int. 1980 Sep;18(3):293–301. doi: 10.1038/ki.1980.139. [DOI] [PubMed] [Google Scholar]
  25. Rayson B. M., Lowther S. O. Steroid regulation of Na+-K+-ATPase: differential sensitivities along the nephron. Am J Physiol. 1984 May;246(5 Pt 2):F656–F662. doi: 10.1152/ajprenal.1984.246.5.F656. [DOI] [PubMed] [Google Scholar]
  26. SIGLER M. H., FORREST J. N., Jr, ELKINTON J. R. RENAL CONCENTRATING ABILITY IN THE ADRENALECTOMIZED RAT. Clin Sci. 1965 Feb;28:29–37. [PubMed] [Google Scholar]
  27. Schafer J. A., Troutman S. L., Andreoli T. E. Volume reabsorption, transepithelial potential differences, and ionic permeability properties in mammalian superficial proximal straight tubules. J Gen Physiol. 1974 Nov;64(5):582–607. doi: 10.1085/jgp.64.5.582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schmidt U., Dubach U. C. The behaviour of Na plus K plus-activated adenosine triphosphatase in various structures of the rat nephron after furosemide application. Nephron. 1970;7(5):447–458. doi: 10.1159/000179844. [DOI] [PubMed] [Google Scholar]
  29. Schmidt U., Schmid J., Schmid H., Dubach U. C. Sodium- and potassium-activated ATPase. A possible target of aldosterone. J Clin Invest. 1975 Mar;55(3):655–660. doi: 10.1172/JCI107973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Schnermann J. Microperfusion study of single short loops of Henle in rat kidney. Pflugers Arch Gesamte Physiol Menschen Tiere. 1968;300(4):255–282. doi: 10.1007/BF00364298. [DOI] [PubMed] [Google Scholar]
  31. Schwartz M. J., Kokko J. P. Urinary concentrating defect of adrenal insufficiency. Permissive role of adrenal steroids on the hydroosmotic response across the rabbit cortical collecting tubule. J Clin Invest. 1980 Aug;66(2):234–242. doi: 10.1172/JCI109849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Stanton B. A., Biemesderfer D., Wade J. B., Giebisch G. Structural and functional study of the rat distal nephron: effects of potassium adaptation and depletion. Kidney Int. 1981 Jan;19(1):36–48. doi: 10.1038/ki.1981.5. [DOI] [PubMed] [Google Scholar]
  33. Stanton B., Giebisch G., Klein-Robbenhaar G., Wade J., DeFronzo R. A. Effects of adrenalectomy and chronic adrenal corticosteroid replacement on potassium transport in rat kidney. J Clin Invest. 1985 Apr;75(4):1317–1326. doi: 10.1172/JCI111832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Stanton B., Giebisch G., Klein-Robbenhaar G., Wade J., DeFronzo R. A. Effects of adrenalectomy and chronic adrenal corticosteroid replacement on potassium transport in rat kidney. J Clin Invest. 1985 Apr;75(4):1317–1326. doi: 10.1172/JCI111832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Welch W. J., Ott C. E., Guthrie G. P., Jr, Kotchen T. A. Renin secretion and loop of Henle chloride reabsorption in the adrenalectomized rat. Am J Physiol. 1985 Oct;249(4 Pt 2):F596–F602. doi: 10.1152/ajprenal.1985.249.4.F596. [DOI] [PubMed] [Google Scholar]
  36. Wiederholt M., Behn C., Schoormans W., Hansen L. Effect of aldosterone on sodium and potassium transport in the kidney. J Steroid Biochem. 1972 Feb;3(2):151–159. doi: 10.1016/0022-4731(72)90045-3. [DOI] [PubMed] [Google Scholar]
  37. Wiederholt M., Wiederholt B. Der Einfluss von Dexamethason auf die Wasser- und Elektrolytausscheidung adrenalektomierter Ratten. Pflugers Arch. 1968;302(1):57–78. doi: 10.1007/BF00586782. [DOI] [PubMed] [Google Scholar]
  38. Wingo C. S. Effect of ouabain on K secretion in cortical collecting tubules from adrenalectomized rabbits. Am J Physiol. 1984 Oct;247(4 Pt 2):F588–F595. doi: 10.1152/ajprenal.1984.247.4.F588. [DOI] [PubMed] [Google Scholar]
  39. Wingo C. S., Kokko J. P., Jacobson H. R. Effects of in vitro aldosterone on the rabbit cortical collecting tubule. Kidney Int. 1985 Jul;28(1):51–57. doi: 10.1038/ki.1985.117. [DOI] [PubMed] [Google Scholar]
  40. Wright F. S., Giebisch G. Renal potassium transport: contributions of individual nephron segments and populations. Am J Physiol. 1978 Dec;235(6):F515–F527. doi: 10.1152/ajprenal.1978.235.6.F515. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES