Abstract
To determine if erythropoietin affects megakaryocytopoiesis, we measured acetylcholinesterase (AchE) activity, a marker of the murine megakaryocytic lineage, after the addition of human recombinant erythropoietin to serumless murine bone marrow cultures. Erythropoietin increased AchE activity substantially. Moreover, when the hormone was added to serumless cultures of 426 isolated single megakaryocytes derived from megakaryocytic colonies, erythropoietin induced a significant increase in the diameters of these cells. From a Bayesian analysis of the likelihood that some megakaryocytes increased in DNA content during the culture period, we estimate that 61% of the cells increased in ploidy. These data indicate that the action of erythropoietin is not restricted to the erythroid lineage.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burstein S. A., Adamson J. W., Thorning D., Harker L. A. Characteristics of murine megakaryocytic colonies in vitro. Blood. 1979 Jul;54(1):169–179. [PubMed] [Google Scholar]
- Burstein S. A., Boyd C. N., Dale G. L. Quantitation of megakaryocytopoiesis in liquid culture by enzymatic determination of acetylcholinesterase. J Cell Physiol. 1985 Jan;122(1):159–165. doi: 10.1002/jcp.1041220124. [DOI] [PubMed] [Google Scholar]
- Chatelain C., Burstein S. A. Fluorescence cytophotometric analysis of megakaryocytic ploidy in culture: studies of normal and thrombocytopenic mice. Blood. 1984 Dec;64(6):1193–1199. [PubMed] [Google Scholar]
- Choi S. I., Simone J. V. Platelet production in experimental iron deficiency anemia. Blood. 1973 Aug;42(2):219–228. [PubMed] [Google Scholar]
- De Gabriele G., Penington D. G. Physiology of the regulation of platelet production. Br J Haematol. 1967 Mar;13(2):202–209. doi: 10.1111/j.1365-2141.1967.tb08732.x. [DOI] [PubMed] [Google Scholar]
- Dukes P. P., Egrie J. C., Strickland T. W., Browne J. K., Lin F. K. Megakaryocyte colony stimulating activity of recombinant human and monkey erythropoietin. Prog Clin Biol Res. 1986;215:105–109. [PubMed] [Google Scholar]
- Evatt B. L., Spivak J. L., Levin J. Relationships between thrombopoiesis and erythropoiesis: with studies of the effects of preparations of thrombopoietin and erythropoietin. Blood. 1976 Oct;48(4):547–558. [PubMed] [Google Scholar]
- Freedman M. H., McDonald T. P., Saunders E. F. Differentiation of murine marrow megakaryocyte progenitors (CFUm): humoral control in vitro. Cell Tissue Kinet. 1981 Jan;14(1):53–58. doi: 10.1111/j.1365-2184.1981.tb00510.x. [DOI] [PubMed] [Google Scholar]
- Hirsh J., Dacie J. V. Persistent post-splenectomy thrombocytosis and thrombo-embolism: a consequence of continuing anaemia. Br J Haematol. 1966 Jan;12(1):44–53. doi: 10.1111/j.1365-2141.1966.tb00125.x. [DOI] [PubMed] [Google Scholar]
- Ishibashi T., Burstein S. A. Interleukin 3 promotes the differentiation of isolated single megakaryocytes. Blood. 1986 May;67(5):1512–1514. [PubMed] [Google Scholar]
- Ishibashi T., Burstein S. A. Separation of murine megakaryocytes and their progenitors on continuous gradients of Percoll. J Cell Physiol. 1985 Dec;125(3):559–566. doi: 10.1002/jcp.1041250327. [DOI] [PubMed] [Google Scholar]
- Jackson C. W., Simone J. V., Edwards C. C. The relationship of anemia and thrombocytosis. J Lab Clin Med. 1974 Sep;84(3):357–368. [PubMed] [Google Scholar]
- Langdon J. R., McDonald T. P. Effects of chronic hypoxia on platelet production in mice. Exp Hematol. 1977 May;5(3):191–198. [PubMed] [Google Scholar]
- Levin J., Levin F. C., Hull D. F., 3rd, Penington D. G. The effects of thrombopoietin on megakaryocyte-cfc, megakaryocytes, and thrombopoiesis: with studies of ploidy and platelet size. Blood. 1982 Oct;60(4):989–998. [PubMed] [Google Scholar]
- Levine R. F., Hazzard K. C., Lamberg J. D. The significance of megakaryocyte size. Blood. 1982 Nov;60(5):1122–1131. [PubMed] [Google Scholar]
- Mazur E. M., Hoffman R., Bruno E. Regulation of human megakaryocytopoiesis. An in vito analysis. J Clin Invest. 1981 Sep;68(3):733–741. doi: 10.1172/JCI110309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McLeod D. L., Shreve M. M., Axelrad A. A. Induction of megakaryocyte colonies with platelet formation in vitro. Nature. 1976 Jun 10;261(5560):492–494. doi: 10.1038/261492a0. [DOI] [PubMed] [Google Scholar]
- Mizoguchi H., Fujiwara Y., Sasaki R., Chiba H. The effect of interleukin 3 and erythropoietin on murine megakaryocyte colony formation. Prog Clin Biol Res. 1986;215:111–115. [PubMed] [Google Scholar]
- Vainchenker W., Bouguet J., Guichard J., Breton-Gorius J. Megakaryocyte colony formation from human bone marrow precursors. Blood. 1979 Oct;54(4):940–945. [PubMed] [Google Scholar]
- Williams N., Jackson H., Iscove N. N., Dukes P. P. The role of erythropoietin, thrombopoietic stimulating factor, and myeloid colony-stimulating factors on murine megakaryocyte colony formation. Exp Hematol. 1984 Oct;12(9):734–740. [PubMed] [Google Scholar]