Abstract
Complementary DNA (cDNA) clones specific for phospholamban of sarcoplasmic reticulum membranes have been isolated from a canine cardiac cDNA library. The amino acid sequence deduced from the cDNA sequence indicates that phospholamban consists of 52 amino acid residues and lacks an amino-terminal signal sequence. The protein has an inferred mol wt 6,080 that is in agreement with its apparent monomeric mol wt 6,000, estimated previously by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Phospholamban contains two distinct domains, a hydrophilic region at the amino terminus (domain I) and a hydrophobic region at the carboxy terminus (domain II). We propose that domain I is localized at the cytoplasmic surface and offers phosphorylatable sites whereas domain II is anchored into the sarcoplasmic reticulum membrane.
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Selected References
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- Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
- Chou P. Y., Fasman G. D. Empirical predictions of protein conformation. Annu Rev Biochem. 1978;47:251–276. doi: 10.1146/annurev.bi.47.070178.001343. [DOI] [PubMed] [Google Scholar]
- Fujii J., Kadoma M., Tada M., Toda H., Sakiyama F. Characterization of structural unit of phospholamban by amino acid sequencing and electrophoretic analysis. Biochem Biophys Res Commun. 1986 Aug 14;138(3):1044–1050. doi: 10.1016/s0006-291x(86)80387-4. [DOI] [PubMed] [Google Scholar]
- Imagawa T., Watanabe T., Nakamura T. Subunit structure and multiple phosphorylation sites of phospholamban. J Biochem. 1986 Jan;99(1):41–53. doi: 10.1093/oxfordjournals.jbchem.a135478. [DOI] [PubMed] [Google Scholar]
- Inui M., Kadoma M., Tada M. Purification and characterization of phospholamban from canine cardiac sarcoplasmic reticulum. J Biol Chem. 1985 Mar 25;260(6):3708–3715. [PubMed] [Google Scholar]
- Jones L. R., Simmerman H. K., Wilson W. W., Gurd F. R., Wegener A. D. Purification and characterization of phospholamban from canine cardiac sarcoplasmic reticulum. J Biol Chem. 1985 Jun 25;260(12):7721–7730. [PubMed] [Google Scholar]
- Kirchberger M. A., Tada M., Katz A. M. Adenosine 3':5'-monophosphate-dependent protein kinase-catalyzed phosphorylation reaction and its relationship to calcium transport in cardiac sarcoplasmic reticulum. J Biol Chem. 1974 Oct 10;249(19):6166–6173. [PubMed] [Google Scholar]
- Kozak M. Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo. Nature. 1984 Mar 15;308(5956):241–246. doi: 10.1038/308241a0. [DOI] [PubMed] [Google Scholar]
- Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
- Le Peuch C. J., Haiech J., Demaille J. G. Concerted regulation of cardiac sarcoplasmic reticulum calcium transport by cyclic adenosine monophosphate dependent and calcium--calmodulin-dependent phosphorylations. Biochemistry. 1979 Nov 13;18(23):5150–5157. doi: 10.1021/bi00590a019. [DOI] [PubMed] [Google Scholar]
- MacLennan D. H., Brandl C. J., Korczak B., Green N. M. Amino-acid sequence of a Ca2+ + Mg2+-dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequence. Nature. 1985 Aug 22;316(6030):696–700. doi: 10.1038/316696a0. [DOI] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Movsesian M. A., Nishikawa M., Adelstein R. S. Phosphorylation of phospholamban by calcium-activated, phospholipid-dependent protein kinase. Stimulation of cardiac sarcoplasmic reticulum calcium uptake. J Biol Chem. 1984 Jul 10;259(13):8029–8032. [PubMed] [Google Scholar]
- Nakazato H., Edmonds M. Purification of messenger RNA and heterogeneous nuclear RNA containing poly(a) sequences. Methods Enzymol. 1974;29:431–443. doi: 10.1016/0076-6879(74)29035-9. [DOI] [PubMed] [Google Scholar]
- Okayama H., Berg P. High-efficiency cloning of full-length cDNA. Mol Cell Biol. 1982 Feb;2(2):161–170. doi: 10.1128/mcb.2.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schiffer M., Edmundson A. B. Use of helical wheels to represent the structures of proteins and to identify segments with helical potential. Biophys J. 1967 Mar;7(2):121–135. doi: 10.1016/S0006-3495(67)86579-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simmerman H. K., Collins J. H., Theibert J. L., Wegener A. D., Jones L. R. Sequence analysis of phospholamban. Identification of phosphorylation sites and two major structural domains. J Biol Chem. 1986 Oct 5;261(28):13333–13341. [PubMed] [Google Scholar]
- Suzuki T., Wang J. H. Stimulation of bovine cardiac sarcoplasmic reticulum Ca2+ pump and blocking of phospholamban phosphorylation and dephosphorylation by a phospholamban monoclonal antibody. J Biol Chem. 1986 May 25;261(15):7018–7023. [PubMed] [Google Scholar]
- Tada M., Inui M., Yamada M., Kadoma M., Kuzuya T., Abe H., Kakiuchi S. Effects of phospholamban phosphorylation catalyzed by adenosine 3':5'-monophosphate- and calmodulin-dependent protein kinases on calcium transport ATPase of cardiac sarcoplasmic reticulum. J Mol Cell Cardiol. 1983 May;15(5):335–346. doi: 10.1016/0022-2828(83)91345-7. [DOI] [PubMed] [Google Scholar]
- Tada M., Katz A. M. Phosphorylation of the sarcoplasmic reticulum and sarcolemma. Annu Rev Physiol. 1982;44:401–423. doi: 10.1146/annurev.ph.44.030182.002153. [DOI] [PubMed] [Google Scholar]
- Tada M., Kirchberger M. A., Katz A. M. Phosphorylation of a 22,000-dalton component of the cardiac sarcoplasmic reticulum by adenosine 3':5'-monophosphate-dependent protein kinase. J Biol Chem. 1975 Apr 10;250(7):2640–2647. [PubMed] [Google Scholar]
- Tada M., Kirchberger M. A., Repke D. I., Katz A. M. The stimulation of calcium transport in cardiac sarcoplasmic reticulum by adenosine 3':5'-monophosphate-dependent protein kinase. J Biol Chem. 1974 Oct 10;249(19):6174–6180. [PubMed] [Google Scholar]
- Tada M., Ohmori F., Yamada M., Abe H. Mechanism of the stimulation of Ca2+-dependent ATPase of cardiac sarcoplasmic reticulum by adenosine 3':5'-monophosphate-dependent protein kinase. Role of the 22,000-dalton protein. J Biol Chem. 1979 Jan 25;254(2):319–326. [PubMed] [Google Scholar]
- Tada M., Yamada M., Ohmori F., Kuzuya T., Inui M., Abe H. Transient state kinetic studies of Ca2+-dependent ATPase and calcium transport by cardiac sarcoplasmic reticulum. Effect of cyclic AMP-dependent protein kinase-catalyzed phosphorylation of phospholamban. J Biol Chem. 1980 Mar 10;255(5):1985–1992. [PubMed] [Google Scholar]
- Wegener A. D., Jones L. R. Phosphorylation-induced mobility shift in phospholamban in sodium dodecyl sulfate-polyacrylamide gels. Evidence for a protein structure consisting of multiple identical phosphorylatable subunits. J Biol Chem. 1984 Feb 10;259(3):1834–1841. [PubMed] [Google Scholar]
- Wegener A. D., Simmerman H. K., Liepnieks J., Jones L. R. Proteolytic cleavage of phospholamban purified from canine cardiac sarcoplasmic reticulum vesicles. Generation of a low resolution model of phospholamban structure. J Biol Chem. 1986 Apr 15;261(11):5154–5159. [PubMed] [Google Scholar]
- Wood W. I., Gitschier J., Lasky L. A., Lawn R. M. Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1585–1588. doi: 10.1073/pnas.82.6.1585. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]