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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Traffic. 2014 Oct 27;15(12):1344–1365. doi: 10.1111/tra.12229

Figure 1. Cu directs the trafficking of endogenous ATP7B in WIF-B cells.

Figure 1

A-C) WIF-B cells incubated overnight in 10 μM BCS to stage endogenous ATP7B at the TGN region. D-F) Cells switched to 10 μM CuCl2 for 60 minutes. G-I) Cu-treated cells that were rinsed and re-incubated in 10 μM BCS for 180 minutes. After fixation, cells were triple-stained with antibodies to ATP7B (green), Syntaxin 6, a post-TGN marker (red) and aminopeptidase N, APN, an apical surface marker (blue). Single confocal planes are shown. J) The fraction of total ATP7B fluorescence that localized to the TGN or apical region in each condition was quantified as the extent of overlap with Syntaxin 6 or APN, respectively. Data shown represent the mean +/- SEM from at least 3 confocal stacks (approximately 28 WIF-B cells/stack, obtained from a single experiment).