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. 1985 Nov;76(5):2026–2029. doi: 10.1172/JCI112204

Immunoreactive corticotropin-releasing factor in human plasma.

T Suda, N Tomori, F Yajima, T Sumitomo, Y Nakagami, T Ushiyama, H Demura, K Shizume
PMCID: PMC424270  PMID: 2997301

Abstract

Plasma immunoreactive corticotropin-releasing factor (I-CRF) levels were determined by using a human CRF radioimmunoassay and an immunoaffinity procedure. The basal plasma I-CRF level in normal subjects was 6 +/- 0.5 pg/ml (mean +/- SD). We found that most plasma I-CRF levels were affected by stress, negative feedback, and circadian rhythm. Basal I-CRF levels were high in patients with Addison's disease, Nelson's syndrome, hypopituitarism stemming from pituitary macroadenoma, and CRF- and adrenocorticotropic hormone-producing tumors. A very low, but significant, amount of I-CRF was detected (1-3 pg/ml) in patients with Cushing's syndrome, in corticosteroid-treated patients, and in a patient with hypothalamic hypopituitarism. These results suggest that a major component of plasma I-CRF is of hypothalamic origin, however, other extrahypothalamic tissues cannot be ruled out as a minor source of plasma I-CRF.

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Selected References

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  1. Bloom F. E., Battenberg E. L., Rivier J., Vale W. Corticotropin releasing factor (CRF): immunoreactive neurones and fibers in rat hypothalamus. Regul Pept. 1982 Jun;4(1):43–48. doi: 10.1016/0167-0115(82)90107-0. [DOI] [PubMed] [Google Scholar]
  2. Bruhn T. O., Sutton R. E., Rivier C. L., Vale W. W. Corticotropin-releasing factor regulates proopiomelanocortin messenger ribonucleic acid levels in vivo. Neuroendocrinology. 1984 Aug;39(2):170–175. doi: 10.1159/000123974. [DOI] [PubMed] [Google Scholar]
  3. Gibbs D. M., Vale W. Presence of corticotropin releasing factor-like immunoreactivity in hypophysial portal blood. Endocrinology. 1982 Oct;111(4):1418–1420. doi: 10.1210/endo-111-4-1418. [DOI] [PubMed] [Google Scholar]
  4. James V. H., Landon J., Wynn V., Greenwood F. C. A fundamental defect of adrenocortical control in Cushing's disease. J Endocrinol. 1968 Jan;40(1):15–28. doi: 10.1677/joe.0.0400015. [DOI] [PubMed] [Google Scholar]
  5. Krieger D. T., Liotta A. S., Suda T., Goodgold A., Condon E. Human plasma immunoreactive lipotropin and adrenocorticotropin in normal subjects and in patients with pituitary-adrenal disease. J Clin Endocrinol Metab. 1979 Apr;48(4):566–571. doi: 10.1210/jcem-48-4-566. [DOI] [PubMed] [Google Scholar]
  6. Mains R. E., Eipper B. A. Biosynthesis of adrenocorticotropic hormone in mouse pituitary tumor cells. J Biol Chem. 1976 Jul 10;251(13):4115–4120. [PubMed] [Google Scholar]
  7. Mouri T., Suda T., Sasano N., Andoh N., Takei Y., Takase M., Sasaki A., Murakami O., Yoshinaga K. Immunocytochemical identification of CRF in the human hypothalamus. Tohoku J Exp Med. 1984 Apr;142(4):423–426. doi: 10.1620/tjem.142.423. [DOI] [PubMed] [Google Scholar]
  8. Orth D. N., Jackson R. V., DeCherney G. S., DeBold C. R., Alexander A. N., Island D. P., Rivier J., Rivier C., Spiess J., Vale W. Effect of synthetic ovine corticotropin-releasing factor. Dose response of plasma adrenocorticotropin and cortisol. J Clin Invest. 1983 Mar;71(3):587–595. doi: 10.1172/JCI110804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rivier J., Spiess J., Vale W. Characterization of rat hypothalamic corticotropin-releasing factor. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4851–4855. doi: 10.1073/pnas.80.15.4851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shibahara S., Morimoto Y., Furutani Y., Notake M., Takahashi H., Shimizu S., Horikawa S., Numa S. Isolation and sequence analysis of the human corticotropin-releasing factor precursor gene. EMBO J. 1983;2(5):775–779. doi: 10.1002/j.1460-2075.1983.tb01499.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Suda T., Tomori N., Tozawa F., Demura H., Shizume K., Mouri T., Miura Y., Sasano N. Immunoreactive corticotropin and corticotropin-releasing factor in human hypothalamus, adrenal, lung cancer, and pheochromocytoma. J Clin Endocrinol Metab. 1984 May;58(5):919–924. doi: 10.1210/jcem-58-5-919. [DOI] [PubMed] [Google Scholar]
  12. Suda T., Tomori N., Tozawa F., Mouri T., Demura H., Shizume K. Distribution and characterization of immunoreactive corticotropin-releasing factor in human tissues. J Clin Endocrinol Metab. 1984 Nov;59(5):861–866. doi: 10.1210/jcem-59-5-861. [DOI] [PubMed] [Google Scholar]
  13. Suda T., Tomori N., Tozawa F., Mouri T., Demura H., Shizume K. Effect of dexamethasone on immunoreactive corticotropin-releasing factor in the rat median eminence and intermediate-posterior pituitary. Endocrinology. 1984 Mar;114(3):851–854. doi: 10.1210/endo-114-3-851. [DOI] [PubMed] [Google Scholar]
  14. Suda T., Tomori N., Tozawa F., Mouri T., Demura H., Shizume K. Effects of bilateral adrenalectomy on immunoreactive corticotropin-releasing factor in the rat median eminence and intermediate-posterior pituitary. Endocrinology. 1983 Sep;113(3):1182–1184. doi: 10.1210/endo-113-3-1182. [DOI] [PubMed] [Google Scholar]
  15. Suda T., Tozawa F., Mouri T., Demura H., Shizume K. Presence of immunoreactive corticotropin-releasing factor in human cerebrospinal fluid. J Clin Endocrinol Metab. 1983 Jul;57(1):225–226. doi: 10.1210/jcem-57-1-225. [DOI] [PubMed] [Google Scholar]
  16. Tomori N., Suda T., Tozawa F., Demura H., Shizume K., Mouri T. Immunoreactive corticotropin-releasing factor concentrations in cerebrospinal fluid from patients with hypothalamic-pituitary-adrenal disorders. J Clin Endocrinol Metab. 1983 Dec;57(6):1305–1307. doi: 10.1210/jcem-57-6-1305. [DOI] [PubMed] [Google Scholar]
  17. Vale W., Spiess J., Rivier C., Rivier J. Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and beta-endorphin. Science. 1981 Sep 18;213(4514):1394–1397. doi: 10.1126/science.6267699. [DOI] [PubMed] [Google Scholar]

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